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Array-Based Screening of Silver Nanoparticle Mineralization Peptides

The use of biomolecules in nanomaterial synthesis has received increasing attention, because they can function as a medium to produce inorganic materials in ambient conditions. Short peptides are putative ligands that interact with metallic surfaces, as they have the potential to control the synthes...

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Autores principales: Tanaka, Masayoshi, Saito, Shogo, Kita, Reo, Jang, Jaehee, Choi, Yonghyun, Choi, Jonghoon, Okochi, Mina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7178033/
https://www.ncbi.nlm.nih.gov/pubmed/32235567
http://dx.doi.org/10.3390/ijms21072377
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author Tanaka, Masayoshi
Saito, Shogo
Kita, Reo
Jang, Jaehee
Choi, Yonghyun
Choi, Jonghoon
Okochi, Mina
author_facet Tanaka, Masayoshi
Saito, Shogo
Kita, Reo
Jang, Jaehee
Choi, Yonghyun
Choi, Jonghoon
Okochi, Mina
author_sort Tanaka, Masayoshi
collection PubMed
description The use of biomolecules in nanomaterial synthesis has received increasing attention, because they can function as a medium to produce inorganic materials in ambient conditions. Short peptides are putative ligands that interact with metallic surfaces, as they have the potential to control the synthesis of nanoscale materials. Silver nanoparticle (AgNP) mineralization using peptides has been investigated; however, further comprehensive analysis must be carried out, because the design of peptide mediated-AgNP properties is still highly challenging. Herein, we employed an array comprising 200 spot synthesis-based peptides, which were previously isolated as gold nanoparticle (AuNP)-binding and/or mineralization peptides, and the AgNP mineralization activity of each peptide was broadly evaluated. Among 10 peptides showing the highest AgNP-synthesis activity (TOP10), nine showed the presence of EE and E[X]E (E: glutamic acid, and X: any amino acid), whereas none of these motifs were found in the WORST25 (25 peptides showing the lowest AgNP synthesis activity) peptides. The size and morphology of the particles synthesized by TOP3 peptides were dependent on their sequences. These results suggested not only that array-based techniques are effective for the peptide screening of AgNP mineralization, but also that AgNP mineralization regulated by peptides has the potential for the synthesis of AgNPs, with controlled morphology in environmentally friendly conditions.
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spelling pubmed-71780332020-04-28 Array-Based Screening of Silver Nanoparticle Mineralization Peptides Tanaka, Masayoshi Saito, Shogo Kita, Reo Jang, Jaehee Choi, Yonghyun Choi, Jonghoon Okochi, Mina Int J Mol Sci Article The use of biomolecules in nanomaterial synthesis has received increasing attention, because they can function as a medium to produce inorganic materials in ambient conditions. Short peptides are putative ligands that interact with metallic surfaces, as they have the potential to control the synthesis of nanoscale materials. Silver nanoparticle (AgNP) mineralization using peptides has been investigated; however, further comprehensive analysis must be carried out, because the design of peptide mediated-AgNP properties is still highly challenging. Herein, we employed an array comprising 200 spot synthesis-based peptides, which were previously isolated as gold nanoparticle (AuNP)-binding and/or mineralization peptides, and the AgNP mineralization activity of each peptide was broadly evaluated. Among 10 peptides showing the highest AgNP-synthesis activity (TOP10), nine showed the presence of EE and E[X]E (E: glutamic acid, and X: any amino acid), whereas none of these motifs were found in the WORST25 (25 peptides showing the lowest AgNP synthesis activity) peptides. The size and morphology of the particles synthesized by TOP3 peptides were dependent on their sequences. These results suggested not only that array-based techniques are effective for the peptide screening of AgNP mineralization, but also that AgNP mineralization regulated by peptides has the potential for the synthesis of AgNPs, with controlled morphology in environmentally friendly conditions. MDPI 2020-03-30 /pmc/articles/PMC7178033/ /pubmed/32235567 http://dx.doi.org/10.3390/ijms21072377 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tanaka, Masayoshi
Saito, Shogo
Kita, Reo
Jang, Jaehee
Choi, Yonghyun
Choi, Jonghoon
Okochi, Mina
Array-Based Screening of Silver Nanoparticle Mineralization Peptides
title Array-Based Screening of Silver Nanoparticle Mineralization Peptides
title_full Array-Based Screening of Silver Nanoparticle Mineralization Peptides
title_fullStr Array-Based Screening of Silver Nanoparticle Mineralization Peptides
title_full_unstemmed Array-Based Screening of Silver Nanoparticle Mineralization Peptides
title_short Array-Based Screening of Silver Nanoparticle Mineralization Peptides
title_sort array-based screening of silver nanoparticle mineralization peptides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7178033/
https://www.ncbi.nlm.nih.gov/pubmed/32235567
http://dx.doi.org/10.3390/ijms21072377
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