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A Novel Ruthenium Based Coordination Compound Against Pathogenic Bacteria
The current epidemic of antibiotic-resistant infections urges to develop alternatives to less-effective antibiotics. To assess anti-bacterial potential, a novel coordinate compound (RU-S4) was synthesized using ruthenium-Schiff base-benzimidazole ligand, where ruthenium chloride was used as the cent...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7178087/ https://www.ncbi.nlm.nih.gov/pubmed/32290291 http://dx.doi.org/10.3390/ijms21072656 |
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author | Sur, Vishma Pratap Mazumdar, Aninda Kopel, Pavel Mukherjee, Soumajit Vítek, Petr Michalkova, Hana Vaculovičová, Markéta Moulick, Amitava |
author_facet | Sur, Vishma Pratap Mazumdar, Aninda Kopel, Pavel Mukherjee, Soumajit Vítek, Petr Michalkova, Hana Vaculovičová, Markéta Moulick, Amitava |
author_sort | Sur, Vishma Pratap |
collection | PubMed |
description | The current epidemic of antibiotic-resistant infections urges to develop alternatives to less-effective antibiotics. To assess anti-bacterial potential, a novel coordinate compound (RU-S4) was synthesized using ruthenium-Schiff base-benzimidazole ligand, where ruthenium chloride was used as the central atom. RU-S4 was characterized by scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDS), and Raman spectroscopy. Antibacterial effect of RU-S4 was studied against Staphylococcus aureus (NCTC 8511), vancomycin-resistant Staphylococcus aureus (VRSA) (CCM 1767), methicillin-resistant Staphylococcus aureus (MRSA) (ST239: SCCmecIIIA), and hospital isolate Staphylococcus epidermidis. The antibacterial activity of RU-S4 was checked by growth curve analysis and the outcome was supported by optical microscopy imaging and fluorescence LIVE/DEAD cell imaging. In vivo (balb/c mice) infection model prepared with VRSA (CCM 1767) and treated with RU-S4. In our experimental conditions, all infected mice were cured. The interaction of coordination compound with bacterial cells were further confirmed by cryo-scanning electron microscope (Cryo-SEM). RU-S4 was completely non-toxic against mammalian cells and in mice and subsequently treated with synthesized RU-S4. |
format | Online Article Text |
id | pubmed-7178087 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71780872020-04-28 A Novel Ruthenium Based Coordination Compound Against Pathogenic Bacteria Sur, Vishma Pratap Mazumdar, Aninda Kopel, Pavel Mukherjee, Soumajit Vítek, Petr Michalkova, Hana Vaculovičová, Markéta Moulick, Amitava Int J Mol Sci Article The current epidemic of antibiotic-resistant infections urges to develop alternatives to less-effective antibiotics. To assess anti-bacterial potential, a novel coordinate compound (RU-S4) was synthesized using ruthenium-Schiff base-benzimidazole ligand, where ruthenium chloride was used as the central atom. RU-S4 was characterized by scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDS), and Raman spectroscopy. Antibacterial effect of RU-S4 was studied against Staphylococcus aureus (NCTC 8511), vancomycin-resistant Staphylococcus aureus (VRSA) (CCM 1767), methicillin-resistant Staphylococcus aureus (MRSA) (ST239: SCCmecIIIA), and hospital isolate Staphylococcus epidermidis. The antibacterial activity of RU-S4 was checked by growth curve analysis and the outcome was supported by optical microscopy imaging and fluorescence LIVE/DEAD cell imaging. In vivo (balb/c mice) infection model prepared with VRSA (CCM 1767) and treated with RU-S4. In our experimental conditions, all infected mice were cured. The interaction of coordination compound with bacterial cells were further confirmed by cryo-scanning electron microscope (Cryo-SEM). RU-S4 was completely non-toxic against mammalian cells and in mice and subsequently treated with synthesized RU-S4. MDPI 2020-04-10 /pmc/articles/PMC7178087/ /pubmed/32290291 http://dx.doi.org/10.3390/ijms21072656 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sur, Vishma Pratap Mazumdar, Aninda Kopel, Pavel Mukherjee, Soumajit Vítek, Petr Michalkova, Hana Vaculovičová, Markéta Moulick, Amitava A Novel Ruthenium Based Coordination Compound Against Pathogenic Bacteria |
title | A Novel Ruthenium Based Coordination Compound Against Pathogenic Bacteria |
title_full | A Novel Ruthenium Based Coordination Compound Against Pathogenic Bacteria |
title_fullStr | A Novel Ruthenium Based Coordination Compound Against Pathogenic Bacteria |
title_full_unstemmed | A Novel Ruthenium Based Coordination Compound Against Pathogenic Bacteria |
title_short | A Novel Ruthenium Based Coordination Compound Against Pathogenic Bacteria |
title_sort | novel ruthenium based coordination compound against pathogenic bacteria |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7178087/ https://www.ncbi.nlm.nih.gov/pubmed/32290291 http://dx.doi.org/10.3390/ijms21072656 |
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