Cargando…

Histone Deacetylase TaHDT701 Functions in TaHDA6-TaHOS15 Complex to Regulate Wheat Defense Responses to Blumeria graminis f.sp. tritici

Powdery mildew disease caused by Blumeria graminis f.sp. tritici (Bgt) leads to severe economic losses in bread wheat (Triticum aestivum L.). To date, only a few epigenetic modulators have been revealed to regulate wheat powdery mildew resistance. In this study, the histone deacetylase 2 (HD2) type...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhi, Pengfei, Kong, Lingyao, Liu, Jiao, Zhang, Xiaona, Wang, Xiaoyu, Li, Haoyu, Sun, Maokai, Li, Yan, Chang, Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7178159/
https://www.ncbi.nlm.nih.gov/pubmed/32290114
http://dx.doi.org/10.3390/ijms21072640
_version_ 1783525391017705472
author Zhi, Pengfei
Kong, Lingyao
Liu, Jiao
Zhang, Xiaona
Wang, Xiaoyu
Li, Haoyu
Sun, Maokai
Li, Yan
Chang, Cheng
author_facet Zhi, Pengfei
Kong, Lingyao
Liu, Jiao
Zhang, Xiaona
Wang, Xiaoyu
Li, Haoyu
Sun, Maokai
Li, Yan
Chang, Cheng
author_sort Zhi, Pengfei
collection PubMed
description Powdery mildew disease caused by Blumeria graminis f.sp. tritici (Bgt) leads to severe economic losses in bread wheat (Triticum aestivum L.). To date, only a few epigenetic modulators have been revealed to regulate wheat powdery mildew resistance. In this study, the histone deacetylase 2 (HD2) type histone deacetylase TaHDT701 was identified as a negative regulator of wheat defense responses to Bgt. Using multiple approaches, we demonstrated that TaHDT701 associates with the RPD3 type histone deacetylase TaHDA6 and the WD40-repeat protein TaHOS15 to constitute a histone deacetylase complex, in which TaHDT701 could stabilize the TaHDA6-TaHOS15 association. Furthermore, knockdown of TaHDT701, TaHDA6, and TaHOS15 resulted in enhanced wheat powdery mildew resistance, suggesting that the TaHDT701-TaHDA6-TaHOS15 histone deacetylase complex negatively regulates wheat defense responses to Bgt. Moreover, chromatin immunoprecipitation assays revealed that TaHDT701 could function in concert with TaHOS15 to recruit TaHDA6 to the promoters of defense-related genes such as TaPR1, TaPR2, TaPR5, and TaWRKY45. In addition, silencing of TaHDT701, TaHDA6, and TaHOS15 resulted in the up-regulation of TaPR1, TaPR2, TaPR5, and TaWRKY45 accompanied with increased histone acetylation and methylation, as well as reduced nucleosome occupancy, at their promoters, suggesting that the TaHDT701-TaHDA6-TaHOS15 histone deacetylase complex suppresses wheat powdery mildew resistance by modulating chromatin state at defense-related genes.
format Online
Article
Text
id pubmed-7178159
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-71781592020-04-28 Histone Deacetylase TaHDT701 Functions in TaHDA6-TaHOS15 Complex to Regulate Wheat Defense Responses to Blumeria graminis f.sp. tritici Zhi, Pengfei Kong, Lingyao Liu, Jiao Zhang, Xiaona Wang, Xiaoyu Li, Haoyu Sun, Maokai Li, Yan Chang, Cheng Int J Mol Sci Article Powdery mildew disease caused by Blumeria graminis f.sp. tritici (Bgt) leads to severe economic losses in bread wheat (Triticum aestivum L.). To date, only a few epigenetic modulators have been revealed to regulate wheat powdery mildew resistance. In this study, the histone deacetylase 2 (HD2) type histone deacetylase TaHDT701 was identified as a negative regulator of wheat defense responses to Bgt. Using multiple approaches, we demonstrated that TaHDT701 associates with the RPD3 type histone deacetylase TaHDA6 and the WD40-repeat protein TaHOS15 to constitute a histone deacetylase complex, in which TaHDT701 could stabilize the TaHDA6-TaHOS15 association. Furthermore, knockdown of TaHDT701, TaHDA6, and TaHOS15 resulted in enhanced wheat powdery mildew resistance, suggesting that the TaHDT701-TaHDA6-TaHOS15 histone deacetylase complex negatively regulates wheat defense responses to Bgt. Moreover, chromatin immunoprecipitation assays revealed that TaHDT701 could function in concert with TaHOS15 to recruit TaHDA6 to the promoters of defense-related genes such as TaPR1, TaPR2, TaPR5, and TaWRKY45. In addition, silencing of TaHDT701, TaHDA6, and TaHOS15 resulted in the up-regulation of TaPR1, TaPR2, TaPR5, and TaWRKY45 accompanied with increased histone acetylation and methylation, as well as reduced nucleosome occupancy, at their promoters, suggesting that the TaHDT701-TaHDA6-TaHOS15 histone deacetylase complex suppresses wheat powdery mildew resistance by modulating chromatin state at defense-related genes. MDPI 2020-04-10 /pmc/articles/PMC7178159/ /pubmed/32290114 http://dx.doi.org/10.3390/ijms21072640 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhi, Pengfei
Kong, Lingyao
Liu, Jiao
Zhang, Xiaona
Wang, Xiaoyu
Li, Haoyu
Sun, Maokai
Li, Yan
Chang, Cheng
Histone Deacetylase TaHDT701 Functions in TaHDA6-TaHOS15 Complex to Regulate Wheat Defense Responses to Blumeria graminis f.sp. tritici
title Histone Deacetylase TaHDT701 Functions in TaHDA6-TaHOS15 Complex to Regulate Wheat Defense Responses to Blumeria graminis f.sp. tritici
title_full Histone Deacetylase TaHDT701 Functions in TaHDA6-TaHOS15 Complex to Regulate Wheat Defense Responses to Blumeria graminis f.sp. tritici
title_fullStr Histone Deacetylase TaHDT701 Functions in TaHDA6-TaHOS15 Complex to Regulate Wheat Defense Responses to Blumeria graminis f.sp. tritici
title_full_unstemmed Histone Deacetylase TaHDT701 Functions in TaHDA6-TaHOS15 Complex to Regulate Wheat Defense Responses to Blumeria graminis f.sp. tritici
title_short Histone Deacetylase TaHDT701 Functions in TaHDA6-TaHOS15 Complex to Regulate Wheat Defense Responses to Blumeria graminis f.sp. tritici
title_sort histone deacetylase tahdt701 functions in tahda6-tahos15 complex to regulate wheat defense responses to blumeria graminis f.sp. tritici
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7178159/
https://www.ncbi.nlm.nih.gov/pubmed/32290114
http://dx.doi.org/10.3390/ijms21072640
work_keys_str_mv AT zhipengfei histonedeacetylasetahdt701functionsintahda6tahos15complextoregulatewheatdefenseresponsestoblumeriagraminisfsptritici
AT konglingyao histonedeacetylasetahdt701functionsintahda6tahos15complextoregulatewheatdefenseresponsestoblumeriagraminisfsptritici
AT liujiao histonedeacetylasetahdt701functionsintahda6tahos15complextoregulatewheatdefenseresponsestoblumeriagraminisfsptritici
AT zhangxiaona histonedeacetylasetahdt701functionsintahda6tahos15complextoregulatewheatdefenseresponsestoblumeriagraminisfsptritici
AT wangxiaoyu histonedeacetylasetahdt701functionsintahda6tahos15complextoregulatewheatdefenseresponsestoblumeriagraminisfsptritici
AT lihaoyu histonedeacetylasetahdt701functionsintahda6tahos15complextoregulatewheatdefenseresponsestoblumeriagraminisfsptritici
AT sunmaokai histonedeacetylasetahdt701functionsintahda6tahos15complextoregulatewheatdefenseresponsestoblumeriagraminisfsptritici
AT liyan histonedeacetylasetahdt701functionsintahda6tahos15complextoregulatewheatdefenseresponsestoblumeriagraminisfsptritici
AT changcheng histonedeacetylasetahdt701functionsintahda6tahos15complextoregulatewheatdefenseresponsestoblumeriagraminisfsptritici