Biomimetic Gold Nanoshell-Loaded Macrophage for Photothermal Biomedicine

The purpose of this study was to investigate the effect of photothermal treatment (PTT) with gold nanoshell (ANS) using a macrophage-mediated delivery system in a head and neck squamous cell carcinoma (HNSCC) cell line. To achieve this, ANS-loaded rat macrophages (ANS-MAs) were prepared via the cocu...

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Autores principales: Kang, Sung Hun, Lee, Yong Kyu, Park, Il Seok, Park, In-Kyu, Hong, Seok Min, Kwon, Soon Young, Choi, Young Hee, Madsen, Steen J., Hirschberg, Henry, Hong, Seok Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7178525/
https://www.ncbi.nlm.nih.gov/pubmed/32352001
http://dx.doi.org/10.1155/2020/5869235
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author Kang, Sung Hun
Lee, Yong Kyu
Park, Il Seok
Park, In-Kyu
Hong, Seok Min
Kwon, Soon Young
Choi, Young Hee
Madsen, Steen J.
Hirschberg, Henry
Hong, Seok Jin
author_facet Kang, Sung Hun
Lee, Yong Kyu
Park, Il Seok
Park, In-Kyu
Hong, Seok Min
Kwon, Soon Young
Choi, Young Hee
Madsen, Steen J.
Hirschberg, Henry
Hong, Seok Jin
author_sort Kang, Sung Hun
collection PubMed
description The purpose of this study was to investigate the effect of photothermal treatment (PTT) with gold nanoshell (ANS) using a macrophage-mediated delivery system in a head and neck squamous cell carcinoma (HNSCC) cell line. To achieve this, ANS-loaded rat macrophages (ANS-MAs) were prepared via the coculture method with ANS. The human HNSCC (FaDu cell) and macrophage (rat macrophage; NR8383 cell) hybrid spheroid models were generated by the centrifugation method to determine the possibility of using ANS-MAs as a cancer therapy. These ANS-MAs were set into the tumor and macrophage hybrid spheroid model to measure PTT efficacy. Kinetic analysis of the spheroid growth pattern revealed that this PTT process caused a decreasing pattern in the volume of the hybrid model containing ANS-MAs (p < 0.001). Comparison with empty macrophages showed harmony between ANS and laser irradiation for the generation of PTT. An annexin V/dead cell marker assay indicated that the PTT-treated hybrid model induced increasing apoptosis and dead cells. Further studies on the toxicity of ANS-MAs are needed to reveal whether it can be considered biocompatible. In summary, the ANS was prepared with a macrophage as the delivery method and protective carrier. The ANS was successfully localized to the macrophages, and their photoabsorption property was stationary. This strategy showed significant growth inhibition of the tumor and macrophage spheroid model under NIR laser irradiation. In vivo toxicology results suggest that ANS-MA is a promising candidate for a biocompatible strategy to overcome the limitations of fabricated nanomaterials. This ANS-MA delivery and PTT strategy may potentially lead to improvements in the quality of life of patients with HNSCC by providing a biocompatible, minimally invasive modality for cancer treatment.
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spelling pubmed-71785252020-04-29 Biomimetic Gold Nanoshell-Loaded Macrophage for Photothermal Biomedicine Kang, Sung Hun Lee, Yong Kyu Park, Il Seok Park, In-Kyu Hong, Seok Min Kwon, Soon Young Choi, Young Hee Madsen, Steen J. Hirschberg, Henry Hong, Seok Jin Biomed Res Int Research Article The purpose of this study was to investigate the effect of photothermal treatment (PTT) with gold nanoshell (ANS) using a macrophage-mediated delivery system in a head and neck squamous cell carcinoma (HNSCC) cell line. To achieve this, ANS-loaded rat macrophages (ANS-MAs) were prepared via the coculture method with ANS. The human HNSCC (FaDu cell) and macrophage (rat macrophage; NR8383 cell) hybrid spheroid models were generated by the centrifugation method to determine the possibility of using ANS-MAs as a cancer therapy. These ANS-MAs were set into the tumor and macrophage hybrid spheroid model to measure PTT efficacy. Kinetic analysis of the spheroid growth pattern revealed that this PTT process caused a decreasing pattern in the volume of the hybrid model containing ANS-MAs (p < 0.001). Comparison with empty macrophages showed harmony between ANS and laser irradiation for the generation of PTT. An annexin V/dead cell marker assay indicated that the PTT-treated hybrid model induced increasing apoptosis and dead cells. Further studies on the toxicity of ANS-MAs are needed to reveal whether it can be considered biocompatible. In summary, the ANS was prepared with a macrophage as the delivery method and protective carrier. The ANS was successfully localized to the macrophages, and their photoabsorption property was stationary. This strategy showed significant growth inhibition of the tumor and macrophage spheroid model under NIR laser irradiation. In vivo toxicology results suggest that ANS-MA is a promising candidate for a biocompatible strategy to overcome the limitations of fabricated nanomaterials. This ANS-MA delivery and PTT strategy may potentially lead to improvements in the quality of life of patients with HNSCC by providing a biocompatible, minimally invasive modality for cancer treatment. Hindawi 2020-04-14 /pmc/articles/PMC7178525/ /pubmed/32352001 http://dx.doi.org/10.1155/2020/5869235 Text en Copyright © 2020 Sung Hun Kang et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kang, Sung Hun
Lee, Yong Kyu
Park, Il Seok
Park, In-Kyu
Hong, Seok Min
Kwon, Soon Young
Choi, Young Hee
Madsen, Steen J.
Hirschberg, Henry
Hong, Seok Jin
Biomimetic Gold Nanoshell-Loaded Macrophage for Photothermal Biomedicine
title Biomimetic Gold Nanoshell-Loaded Macrophage for Photothermal Biomedicine
title_full Biomimetic Gold Nanoshell-Loaded Macrophage for Photothermal Biomedicine
title_fullStr Biomimetic Gold Nanoshell-Loaded Macrophage for Photothermal Biomedicine
title_full_unstemmed Biomimetic Gold Nanoshell-Loaded Macrophage for Photothermal Biomedicine
title_short Biomimetic Gold Nanoshell-Loaded Macrophage for Photothermal Biomedicine
title_sort biomimetic gold nanoshell-loaded macrophage for photothermal biomedicine
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7178525/
https://www.ncbi.nlm.nih.gov/pubmed/32352001
http://dx.doi.org/10.1155/2020/5869235
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