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Sensitivity of Polyvoltine Thai Strains of Bombyx mori to a BmNPV Isolate From Mahasarakham

Virus infection by the Bombyx mori nucleopolyhedrovirus (BmNPV) is the most severe disease in Thai sericultural practice of polyvoltine silkworms. Here, we characterized a newly isolated BmNPV isolated from the Mahasarakham province in Thailand (BmNPV-MSU). The purity and morphology of BmNPV-MSU wer...

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Autores principales: Suraporn, Siripuk, Terenius, Olle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7178808/
https://www.ncbi.nlm.nih.gov/pubmed/32324213
http://dx.doi.org/10.1093/jisesa/ieaa023
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author Suraporn, Siripuk
Terenius, Olle
author_facet Suraporn, Siripuk
Terenius, Olle
author_sort Suraporn, Siripuk
collection PubMed
description Virus infection by the Bombyx mori nucleopolyhedrovirus (BmNPV) is the most severe disease in Thai sericultural practice of polyvoltine silkworms. Here, we characterized a newly isolated BmNPV isolated from the Mahasarakham province in Thailand (BmNPV-MSU). The purity and morphology of BmNPV-MSU were examined using light microscopy and scanning electron microscopy. The polyhedral inclusion bodies (PIBs) of BmNPV-MSU appeared in tetragonal, hexagonal, octagonal, and globular forms. The virions were both single and multiple embedded as observed by transmission electron microscopy. We also determined the virulence of BmNPV-MSU for six different Thai polyvoltine strains by LC(50) and time to death after infection. The LC(50) values of Nang Lai, NK04, and Sam Rong strains were 5.05–1.52 × 10(7) PIBs per ml and mortality peaked 7- to 8-d after inoculation. For Nang Noi, SP2, and RE05 strains the LC(50) values were 7.91–1.82 × 10(6) PIBs/ml and mortality peaked 4–5 d after inoculation, thus having lower chance of survival to infection by BmNPV-MSU.
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spelling pubmed-71788082020-04-28 Sensitivity of Polyvoltine Thai Strains of Bombyx mori to a BmNPV Isolate From Mahasarakham Suraporn, Siripuk Terenius, Olle J Insect Sci Research Virus infection by the Bombyx mori nucleopolyhedrovirus (BmNPV) is the most severe disease in Thai sericultural practice of polyvoltine silkworms. Here, we characterized a newly isolated BmNPV isolated from the Mahasarakham province in Thailand (BmNPV-MSU). The purity and morphology of BmNPV-MSU were examined using light microscopy and scanning electron microscopy. The polyhedral inclusion bodies (PIBs) of BmNPV-MSU appeared in tetragonal, hexagonal, octagonal, and globular forms. The virions were both single and multiple embedded as observed by transmission electron microscopy. We also determined the virulence of BmNPV-MSU for six different Thai polyvoltine strains by LC(50) and time to death after infection. The LC(50) values of Nang Lai, NK04, and Sam Rong strains were 5.05–1.52 × 10(7) PIBs per ml and mortality peaked 7- to 8-d after inoculation. For Nang Noi, SP2, and RE05 strains the LC(50) values were 7.91–1.82 × 10(6) PIBs/ml and mortality peaked 4–5 d after inoculation, thus having lower chance of survival to infection by BmNPV-MSU. Oxford University Press 2020-04-23 /pmc/articles/PMC7178808/ /pubmed/32324213 http://dx.doi.org/10.1093/jisesa/ieaa023 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of Entomological Society of America. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Suraporn, Siripuk
Terenius, Olle
Sensitivity of Polyvoltine Thai Strains of Bombyx mori to a BmNPV Isolate From Mahasarakham
title Sensitivity of Polyvoltine Thai Strains of Bombyx mori to a BmNPV Isolate From Mahasarakham
title_full Sensitivity of Polyvoltine Thai Strains of Bombyx mori to a BmNPV Isolate From Mahasarakham
title_fullStr Sensitivity of Polyvoltine Thai Strains of Bombyx mori to a BmNPV Isolate From Mahasarakham
title_full_unstemmed Sensitivity of Polyvoltine Thai Strains of Bombyx mori to a BmNPV Isolate From Mahasarakham
title_short Sensitivity of Polyvoltine Thai Strains of Bombyx mori to a BmNPV Isolate From Mahasarakham
title_sort sensitivity of polyvoltine thai strains of bombyx mori to a bmnpv isolate from mahasarakham
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7178808/
https://www.ncbi.nlm.nih.gov/pubmed/32324213
http://dx.doi.org/10.1093/jisesa/ieaa023
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