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Inhibition of African swine fever virus protease by myricetin and myricitrin
African swine fever (ASF) caused by the ASF virus (ASFV) is the most hazardous swine disease. Since a huge number of pigs have been slaughtered to avoid a pandemic spread, intense studies on the disease should be followed quickly. Recent studies reported that flavonoids have various antiviral activi...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7178854/ https://www.ncbi.nlm.nih.gov/pubmed/32299265 http://dx.doi.org/10.1080/14756366.2020.1754813 |
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author | Jo, Seri Kim, Suwon Shin, Dong Hae Kim, Mi-Sun |
author_facet | Jo, Seri Kim, Suwon Shin, Dong Hae Kim, Mi-Sun |
author_sort | Jo, Seri |
collection | PubMed |
description | African swine fever (ASF) caused by the ASF virus (ASFV) is the most hazardous swine disease. Since a huge number of pigs have been slaughtered to avoid a pandemic spread, intense studies on the disease should be followed quickly. Recent studies reported that flavonoids have various antiviral activity including ASFV. In this report, ASFV protease was selected as an antiviral target protein to cope with ASF. With a FRET (Fluorescence resonance energy transfer) method, ASFV protease was assayed with a flavonoid library which was composed of sixty-five derivatives classified based on ten different scaffolds. Of these, the flavonols scaffold contains a potential anti-ASFV protease activity. The most prominent flavonol was myricetin with IC(50) of 8.4 μM. Its derivative, myricitrin, with the rhamnoside moiety was also showed the profound inhibitory effect on ASFV protease. These two flavonols apparently provide a way to develop anti-ASFV agents based on their scaffold. |
format | Online Article Text |
id | pubmed-7178854 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-71788542020-05-01 Inhibition of African swine fever virus protease by myricetin and myricitrin Jo, Seri Kim, Suwon Shin, Dong Hae Kim, Mi-Sun J Enzyme Inhib Med Chem Research Paper African swine fever (ASF) caused by the ASF virus (ASFV) is the most hazardous swine disease. Since a huge number of pigs have been slaughtered to avoid a pandemic spread, intense studies on the disease should be followed quickly. Recent studies reported that flavonoids have various antiviral activity including ASFV. In this report, ASFV protease was selected as an antiviral target protein to cope with ASF. With a FRET (Fluorescence resonance energy transfer) method, ASFV protease was assayed with a flavonoid library which was composed of sixty-five derivatives classified based on ten different scaffolds. Of these, the flavonols scaffold contains a potential anti-ASFV protease activity. The most prominent flavonol was myricetin with IC(50) of 8.4 μM. Its derivative, myricitrin, with the rhamnoside moiety was also showed the profound inhibitory effect on ASFV protease. These two flavonols apparently provide a way to develop anti-ASFV agents based on their scaffold. Taylor & Francis 2020-04-17 /pmc/articles/PMC7178854/ /pubmed/32299265 http://dx.doi.org/10.1080/14756366.2020.1754813 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Jo, Seri Kim, Suwon Shin, Dong Hae Kim, Mi-Sun Inhibition of African swine fever virus protease by myricetin and myricitrin |
title | Inhibition of African swine fever virus protease by myricetin and myricitrin |
title_full | Inhibition of African swine fever virus protease by myricetin and myricitrin |
title_fullStr | Inhibition of African swine fever virus protease by myricetin and myricitrin |
title_full_unstemmed | Inhibition of African swine fever virus protease by myricetin and myricitrin |
title_short | Inhibition of African swine fever virus protease by myricetin and myricitrin |
title_sort | inhibition of african swine fever virus protease by myricetin and myricitrin |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7178854/ https://www.ncbi.nlm.nih.gov/pubmed/32299265 http://dx.doi.org/10.1080/14756366.2020.1754813 |
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