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Identification of the Molecular Mechanisms of Peimine in the Treatment of Cough Using Computational Target Fishing
Peimine (also known as verticine) is the major bioactive and characterized compound of Fritillariae Thunbergii Bulbus, a traditional Chinese medicine that is most frequently used to relieve a cough. Nevertheless, its molecular targets and mechanisms of action for cough are still not clear. In the pr...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7179178/ https://www.ncbi.nlm.nih.gov/pubmed/32131410 http://dx.doi.org/10.3390/molecules25051105 |
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author | Zhang, Lihua Cui, Mingchao Chen, Shaojun |
author_facet | Zhang, Lihua Cui, Mingchao Chen, Shaojun |
author_sort | Zhang, Lihua |
collection | PubMed |
description | Peimine (also known as verticine) is the major bioactive and characterized compound of Fritillariae Thunbergii Bulbus, a traditional Chinese medicine that is most frequently used to relieve a cough. Nevertheless, its molecular targets and mechanisms of action for cough are still not clear. In the present study, potential targets of peimine for cough were identified using computational target fishing combined with manual database mining. In addition, protein-protein interaction (PPI), gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed using, GeneMANIA and Database for Annotation, Visualization and Integrated Discovery (DAVID) databases respectively. Finally, an interaction network of drug-targets-pathways was constructed using Cytoscape. The results identified 23 potential targets of peimine associated with cough, and suggested that MAPK1, AKT1 and PPKCB may be important targets of pemine for the treatment of cough. The functional annotations of protein targets were related to the regulation of immunological and neurological function through specific biological processes and related pathways. A visual representation of the multiple targets and pathways that form a network underlying the systematic actions of peimine was generated. In summary, peimine is predicted to exert its systemic pharmacological effects on cough by targeting a network composed of multiple proteins and pathways. |
format | Online Article Text |
id | pubmed-7179178 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71791782020-04-28 Identification of the Molecular Mechanisms of Peimine in the Treatment of Cough Using Computational Target Fishing Zhang, Lihua Cui, Mingchao Chen, Shaojun Molecules Article Peimine (also known as verticine) is the major bioactive and characterized compound of Fritillariae Thunbergii Bulbus, a traditional Chinese medicine that is most frequently used to relieve a cough. Nevertheless, its molecular targets and mechanisms of action for cough are still not clear. In the present study, potential targets of peimine for cough were identified using computational target fishing combined with manual database mining. In addition, protein-protein interaction (PPI), gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed using, GeneMANIA and Database for Annotation, Visualization and Integrated Discovery (DAVID) databases respectively. Finally, an interaction network of drug-targets-pathways was constructed using Cytoscape. The results identified 23 potential targets of peimine associated with cough, and suggested that MAPK1, AKT1 and PPKCB may be important targets of pemine for the treatment of cough. The functional annotations of protein targets were related to the regulation of immunological and neurological function through specific biological processes and related pathways. A visual representation of the multiple targets and pathways that form a network underlying the systematic actions of peimine was generated. In summary, peimine is predicted to exert its systemic pharmacological effects on cough by targeting a network composed of multiple proteins and pathways. MDPI 2020-03-02 /pmc/articles/PMC7179178/ /pubmed/32131410 http://dx.doi.org/10.3390/molecules25051105 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Lihua Cui, Mingchao Chen, Shaojun Identification of the Molecular Mechanisms of Peimine in the Treatment of Cough Using Computational Target Fishing |
title | Identification of the Molecular Mechanisms of Peimine in the Treatment of Cough Using Computational Target Fishing |
title_full | Identification of the Molecular Mechanisms of Peimine in the Treatment of Cough Using Computational Target Fishing |
title_fullStr | Identification of the Molecular Mechanisms of Peimine in the Treatment of Cough Using Computational Target Fishing |
title_full_unstemmed | Identification of the Molecular Mechanisms of Peimine in the Treatment of Cough Using Computational Target Fishing |
title_short | Identification of the Molecular Mechanisms of Peimine in the Treatment of Cough Using Computational Target Fishing |
title_sort | identification of the molecular mechanisms of peimine in the treatment of cough using computational target fishing |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7179178/ https://www.ncbi.nlm.nih.gov/pubmed/32131410 http://dx.doi.org/10.3390/molecules25051105 |
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