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Gintonin-Enriched Fraction Suppresses Heat Stress-Induced Inflammation through LPA Receptor

Heat stress can be caused by various environmental factors. When exposed to heat stress, oxidative stress and inflammatory reaction occur due to an increase of reactive oxygen species (ROS) in the body. In particular, inflammatory responses induced by heat stress are common in muscle cells, which ar...

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Autores principales: Chei, Sungwoo, Song, Ji-Hyeon, Oh, Hyun-Ji, Lee, Kippeum, Jin, Heegu, Choi, Sun-Hye, Nah, Seung-Yeol, Lee, Boo-Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7179209/
https://www.ncbi.nlm.nih.gov/pubmed/32106493
http://dx.doi.org/10.3390/molecules25051019
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author Chei, Sungwoo
Song, Ji-Hyeon
Oh, Hyun-Ji
Lee, Kippeum
Jin, Heegu
Choi, Sun-Hye
Nah, Seung-Yeol
Lee, Boo-Yong
author_facet Chei, Sungwoo
Song, Ji-Hyeon
Oh, Hyun-Ji
Lee, Kippeum
Jin, Heegu
Choi, Sun-Hye
Nah, Seung-Yeol
Lee, Boo-Yong
author_sort Chei, Sungwoo
collection PubMed
description Heat stress can be caused by various environmental factors. When exposed to heat stress, oxidative stress and inflammatory reaction occur due to an increase of reactive oxygen species (ROS) in the body. In particular, inflammatory responses induced by heat stress are common in muscle cells, which are the most exposed to heat stress and directly affected. Gintonin-Enriched Fraction (GEF) is a non-saponin component of ginseng, a glycolipoprotein. It is known that it has excellent neuroprotective effects, therefore, we aimed to confirm the protective effect against heat stress by using GEF. C2C12 cells were exposed to high temperature stress for 1, 12 and 15 h, and the expression of signals was analyzed over time. Changes in the expression of the factors that were observed under heat stress were confirmed at the protein level. Exposure to heat stress increases phosphorylation of p38 and extracellular signal-regulated kinase (ERK) and increases expression of inflammatory factors such as NLRP3 inflammasome through lysophosphatidic acid (LPA) receptor. Activated inflammatory signals also increase the secretion of inflammatory cytokines such as interleukin 6 (IL-6) and interleukin 18 (IL-18). Also, expression of glutathione reductase (GR) and catalase related to oxidative stress is increased. However, it was confirmed that the changes due to the heat stress were suppressed by the GEF treatment. Therefore, we suggest that GEF helps to protect heat stress in muscle cell and prevent tissue damage by oxidative stress and inflammation.
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spelling pubmed-71792092020-04-28 Gintonin-Enriched Fraction Suppresses Heat Stress-Induced Inflammation through LPA Receptor Chei, Sungwoo Song, Ji-Hyeon Oh, Hyun-Ji Lee, Kippeum Jin, Heegu Choi, Sun-Hye Nah, Seung-Yeol Lee, Boo-Yong Molecules Article Heat stress can be caused by various environmental factors. When exposed to heat stress, oxidative stress and inflammatory reaction occur due to an increase of reactive oxygen species (ROS) in the body. In particular, inflammatory responses induced by heat stress are common in muscle cells, which are the most exposed to heat stress and directly affected. Gintonin-Enriched Fraction (GEF) is a non-saponin component of ginseng, a glycolipoprotein. It is known that it has excellent neuroprotective effects, therefore, we aimed to confirm the protective effect against heat stress by using GEF. C2C12 cells were exposed to high temperature stress for 1, 12 and 15 h, and the expression of signals was analyzed over time. Changes in the expression of the factors that were observed under heat stress were confirmed at the protein level. Exposure to heat stress increases phosphorylation of p38 and extracellular signal-regulated kinase (ERK) and increases expression of inflammatory factors such as NLRP3 inflammasome through lysophosphatidic acid (LPA) receptor. Activated inflammatory signals also increase the secretion of inflammatory cytokines such as interleukin 6 (IL-6) and interleukin 18 (IL-18). Also, expression of glutathione reductase (GR) and catalase related to oxidative stress is increased. However, it was confirmed that the changes due to the heat stress were suppressed by the GEF treatment. Therefore, we suggest that GEF helps to protect heat stress in muscle cell and prevent tissue damage by oxidative stress and inflammation. MDPI 2020-02-25 /pmc/articles/PMC7179209/ /pubmed/32106493 http://dx.doi.org/10.3390/molecules25051019 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chei, Sungwoo
Song, Ji-Hyeon
Oh, Hyun-Ji
Lee, Kippeum
Jin, Heegu
Choi, Sun-Hye
Nah, Seung-Yeol
Lee, Boo-Yong
Gintonin-Enriched Fraction Suppresses Heat Stress-Induced Inflammation through LPA Receptor
title Gintonin-Enriched Fraction Suppresses Heat Stress-Induced Inflammation through LPA Receptor
title_full Gintonin-Enriched Fraction Suppresses Heat Stress-Induced Inflammation through LPA Receptor
title_fullStr Gintonin-Enriched Fraction Suppresses Heat Stress-Induced Inflammation through LPA Receptor
title_full_unstemmed Gintonin-Enriched Fraction Suppresses Heat Stress-Induced Inflammation through LPA Receptor
title_short Gintonin-Enriched Fraction Suppresses Heat Stress-Induced Inflammation through LPA Receptor
title_sort gintonin-enriched fraction suppresses heat stress-induced inflammation through lpa receptor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7179209/
https://www.ncbi.nlm.nih.gov/pubmed/32106493
http://dx.doi.org/10.3390/molecules25051019
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