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Interaction between hydrogen sulfide, nitric oxide, and carbon monoxide pathways in the bovine isolated retina
PURPOSE: Nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H(2)S) are physiologically relevant gaseous neurotransmitters that are endogenously produced in mammalian tissues. In the present study, we investigated the possibility that NO and CO can regulate the endogenous levels of H(2)S i...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AIMS Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7179363/ https://www.ncbi.nlm.nih.gov/pubmed/32341971 http://dx.doi.org/10.3934/Neuroscience.2019.3.104 |
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author | Kulkarni-Chitnis, Madhura Mitchell-Bush, Leah Belford, Remmington Robinson, Jenaye Opere, Catherine A. Ohia, Sunny E. Mbye, Ya Fatou N. |
author_facet | Kulkarni-Chitnis, Madhura Mitchell-Bush, Leah Belford, Remmington Robinson, Jenaye Opere, Catherine A. Ohia, Sunny E. Mbye, Ya Fatou N. |
author_sort | Kulkarni-Chitnis, Madhura |
collection | PubMed |
description | PURPOSE: Nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H(2)S) are physiologically relevant gaseous neurotransmitters that are endogenously produced in mammalian tissues. In the present study, we investigated the possibility that NO and CO can regulate the endogenous levels of H(2)S in bovine isolated neural retina. METHODS: Isolated bovine neural retina were homogenized and tissue homogenates were treated with a NO synthase inhibitor, NO donor, heme oxygenase-1 inhibitor, and/donor. H(2)S concentrations in bovine retinal homogenates were measured using a well-established colorimetric assay. RESULTS: L-NAME (300 nM–500 µM) caused a concentration-dependent decrease in basal endogenous levels of H(2)S by 86.2%. On the other hand, SNP (10–300 µM) elicited a concentration-related increase in H(2)S levels from 18.3 nM/mg of protein to 65.7 nM/mg of protein. ZnPP-IX (300 nM–10 µM) caused a concentration-dependent increase in the endogenous production of H(2)S whereas hemin (300 nM–20 µM) attenuated the basal levels of H(2)S. CONCLUSION: We conclude that changes in the biosynthesis and availability of both NO and CO can interfere with the pathway/s involved in the production of H(2)S in the retina. The demonstrated ability of NO, CO and H(2)S to interact in the mammalian retina affirms a physiological/pharmacological role for these gaseous mediators in the eye. |
format | Online Article Text |
id | pubmed-7179363 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | AIMS Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-71793632020-04-27 Interaction between hydrogen sulfide, nitric oxide, and carbon monoxide pathways in the bovine isolated retina Kulkarni-Chitnis, Madhura Mitchell-Bush, Leah Belford, Remmington Robinson, Jenaye Opere, Catherine A. Ohia, Sunny E. Mbye, Ya Fatou N. AIMS Neurosci Research Article PURPOSE: Nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H(2)S) are physiologically relevant gaseous neurotransmitters that are endogenously produced in mammalian tissues. In the present study, we investigated the possibility that NO and CO can regulate the endogenous levels of H(2)S in bovine isolated neural retina. METHODS: Isolated bovine neural retina were homogenized and tissue homogenates were treated with a NO synthase inhibitor, NO donor, heme oxygenase-1 inhibitor, and/donor. H(2)S concentrations in bovine retinal homogenates were measured using a well-established colorimetric assay. RESULTS: L-NAME (300 nM–500 µM) caused a concentration-dependent decrease in basal endogenous levels of H(2)S by 86.2%. On the other hand, SNP (10–300 µM) elicited a concentration-related increase in H(2)S levels from 18.3 nM/mg of protein to 65.7 nM/mg of protein. ZnPP-IX (300 nM–10 µM) caused a concentration-dependent increase in the endogenous production of H(2)S whereas hemin (300 nM–20 µM) attenuated the basal levels of H(2)S. CONCLUSION: We conclude that changes in the biosynthesis and availability of both NO and CO can interfere with the pathway/s involved in the production of H(2)S in the retina. The demonstrated ability of NO, CO and H(2)S to interact in the mammalian retina affirms a physiological/pharmacological role for these gaseous mediators in the eye. AIMS Press 2019-06-18 /pmc/articles/PMC7179363/ /pubmed/32341971 http://dx.doi.org/10.3934/Neuroscience.2019.3.104 Text en © 2019 the Author(s), licensee AIMS Press This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) |
spellingShingle | Research Article Kulkarni-Chitnis, Madhura Mitchell-Bush, Leah Belford, Remmington Robinson, Jenaye Opere, Catherine A. Ohia, Sunny E. Mbye, Ya Fatou N. Interaction between hydrogen sulfide, nitric oxide, and carbon monoxide pathways in the bovine isolated retina |
title | Interaction between hydrogen sulfide, nitric oxide, and carbon monoxide pathways in the bovine isolated retina |
title_full | Interaction between hydrogen sulfide, nitric oxide, and carbon monoxide pathways in the bovine isolated retina |
title_fullStr | Interaction between hydrogen sulfide, nitric oxide, and carbon monoxide pathways in the bovine isolated retina |
title_full_unstemmed | Interaction between hydrogen sulfide, nitric oxide, and carbon monoxide pathways in the bovine isolated retina |
title_short | Interaction between hydrogen sulfide, nitric oxide, and carbon monoxide pathways in the bovine isolated retina |
title_sort | interaction between hydrogen sulfide, nitric oxide, and carbon monoxide pathways in the bovine isolated retina |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7179363/ https://www.ncbi.nlm.nih.gov/pubmed/32341971 http://dx.doi.org/10.3934/Neuroscience.2019.3.104 |
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