Cargando…

Interaction between hydrogen sulfide, nitric oxide, and carbon monoxide pathways in the bovine isolated retina

PURPOSE: Nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H(2)S) are physiologically relevant gaseous neurotransmitters that are endogenously produced in mammalian tissues. In the present study, we investigated the possibility that NO and CO can regulate the endogenous levels of H(2)S i...

Descripción completa

Detalles Bibliográficos
Autores principales: Kulkarni-Chitnis, Madhura, Mitchell-Bush, Leah, Belford, Remmington, Robinson, Jenaye, Opere, Catherine A., Ohia, Sunny E., Mbye, Ya Fatou N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIMS Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7179363/
https://www.ncbi.nlm.nih.gov/pubmed/32341971
http://dx.doi.org/10.3934/Neuroscience.2019.3.104
_version_ 1783525642497687552
author Kulkarni-Chitnis, Madhura
Mitchell-Bush, Leah
Belford, Remmington
Robinson, Jenaye
Opere, Catherine A.
Ohia, Sunny E.
Mbye, Ya Fatou N.
author_facet Kulkarni-Chitnis, Madhura
Mitchell-Bush, Leah
Belford, Remmington
Robinson, Jenaye
Opere, Catherine A.
Ohia, Sunny E.
Mbye, Ya Fatou N.
author_sort Kulkarni-Chitnis, Madhura
collection PubMed
description PURPOSE: Nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H(2)S) are physiologically relevant gaseous neurotransmitters that are endogenously produced in mammalian tissues. In the present study, we investigated the possibility that NO and CO can regulate the endogenous levels of H(2)S in bovine isolated neural retina. METHODS: Isolated bovine neural retina were homogenized and tissue homogenates were treated with a NO synthase inhibitor, NO donor, heme oxygenase-1 inhibitor, and/donor. H(2)S concentrations in bovine retinal homogenates were measured using a well-established colorimetric assay. RESULTS: L-NAME (300 nM–500 µM) caused a concentration-dependent decrease in basal endogenous levels of H(2)S by 86.2%. On the other hand, SNP (10–300 µM) elicited a concentration-related increase in H(2)S levels from 18.3 nM/mg of protein to 65.7 nM/mg of protein. ZnPP-IX (300 nM–10 µM) caused a concentration-dependent increase in the endogenous production of H(2)S whereas hemin (300 nM–20 µM) attenuated the basal levels of H(2)S. CONCLUSION: We conclude that changes in the biosynthesis and availability of both NO and CO can interfere with the pathway/s involved in the production of H(2)S in the retina. The demonstrated ability of NO, CO and H(2)S to interact in the mammalian retina affirms a physiological/pharmacological role for these gaseous mediators in the eye.
format Online
Article
Text
id pubmed-7179363
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher AIMS Press
record_format MEDLINE/PubMed
spelling pubmed-71793632020-04-27 Interaction between hydrogen sulfide, nitric oxide, and carbon monoxide pathways in the bovine isolated retina Kulkarni-Chitnis, Madhura Mitchell-Bush, Leah Belford, Remmington Robinson, Jenaye Opere, Catherine A. Ohia, Sunny E. Mbye, Ya Fatou N. AIMS Neurosci Research Article PURPOSE: Nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H(2)S) are physiologically relevant gaseous neurotransmitters that are endogenously produced in mammalian tissues. In the present study, we investigated the possibility that NO and CO can regulate the endogenous levels of H(2)S in bovine isolated neural retina. METHODS: Isolated bovine neural retina were homogenized and tissue homogenates were treated with a NO synthase inhibitor, NO donor, heme oxygenase-1 inhibitor, and/donor. H(2)S concentrations in bovine retinal homogenates were measured using a well-established colorimetric assay. RESULTS: L-NAME (300 nM–500 µM) caused a concentration-dependent decrease in basal endogenous levels of H(2)S by 86.2%. On the other hand, SNP (10–300 µM) elicited a concentration-related increase in H(2)S levels from 18.3 nM/mg of protein to 65.7 nM/mg of protein. ZnPP-IX (300 nM–10 µM) caused a concentration-dependent increase in the endogenous production of H(2)S whereas hemin (300 nM–20 µM) attenuated the basal levels of H(2)S. CONCLUSION: We conclude that changes in the biosynthesis and availability of both NO and CO can interfere with the pathway/s involved in the production of H(2)S in the retina. The demonstrated ability of NO, CO and H(2)S to interact in the mammalian retina affirms a physiological/pharmacological role for these gaseous mediators in the eye. AIMS Press 2019-06-18 /pmc/articles/PMC7179363/ /pubmed/32341971 http://dx.doi.org/10.3934/Neuroscience.2019.3.104 Text en © 2019 the Author(s), licensee AIMS Press This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)
spellingShingle Research Article
Kulkarni-Chitnis, Madhura
Mitchell-Bush, Leah
Belford, Remmington
Robinson, Jenaye
Opere, Catherine A.
Ohia, Sunny E.
Mbye, Ya Fatou N.
Interaction between hydrogen sulfide, nitric oxide, and carbon monoxide pathways in the bovine isolated retina
title Interaction between hydrogen sulfide, nitric oxide, and carbon monoxide pathways in the bovine isolated retina
title_full Interaction between hydrogen sulfide, nitric oxide, and carbon monoxide pathways in the bovine isolated retina
title_fullStr Interaction between hydrogen sulfide, nitric oxide, and carbon monoxide pathways in the bovine isolated retina
title_full_unstemmed Interaction between hydrogen sulfide, nitric oxide, and carbon monoxide pathways in the bovine isolated retina
title_short Interaction between hydrogen sulfide, nitric oxide, and carbon monoxide pathways in the bovine isolated retina
title_sort interaction between hydrogen sulfide, nitric oxide, and carbon monoxide pathways in the bovine isolated retina
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7179363/
https://www.ncbi.nlm.nih.gov/pubmed/32341971
http://dx.doi.org/10.3934/Neuroscience.2019.3.104
work_keys_str_mv AT kulkarnichitnismadhura interactionbetweenhydrogensulfidenitricoxideandcarbonmonoxidepathwaysinthebovineisolatedretina
AT mitchellbushleah interactionbetweenhydrogensulfidenitricoxideandcarbonmonoxidepathwaysinthebovineisolatedretina
AT belfordremmington interactionbetweenhydrogensulfidenitricoxideandcarbonmonoxidepathwaysinthebovineisolatedretina
AT robinsonjenaye interactionbetweenhydrogensulfidenitricoxideandcarbonmonoxidepathwaysinthebovineisolatedretina
AT operecatherinea interactionbetweenhydrogensulfidenitricoxideandcarbonmonoxidepathwaysinthebovineisolatedretina
AT ohiasunnye interactionbetweenhydrogensulfidenitricoxideandcarbonmonoxidepathwaysinthebovineisolatedretina
AT mbyeyafatoun interactionbetweenhydrogensulfidenitricoxideandcarbonmonoxidepathwaysinthebovineisolatedretina