Scope and Predictive Genetic/Phenotypic Signatures of Bicarbonate (NaHCO(3)) Responsiveness and β-Lactam Sensitization in Methicillin-Resistant Staphylococcus aureus

Addition of sodium bicarbonate (NaHCO(3)) to standard antimicrobial susceptibility testing medium reveals certain methicillin-resistant Staphylococcus aureus (MRSA) strains to be highly susceptible to β-lactams. We investigated the prevalence of this phenotype (NaHCO(3) responsiveness) to two β-lact...

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Detalles Bibliográficos
Autores principales: Ersoy, Selvi C., Otmishi, Mariam, Milan, Vanessa T., Li, Liang, Pak, Youngju, Mediavilla, Jose, Chen, Liang, Kreiswirth, Barry, Chambers, Henry F., Proctor, Richard A., Xiong, Yan Q., Fowler, Vance G., Bayer, Arnold S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2020
Materias:
Susceptibility
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7179597/
https://www.ncbi.nlm.nih.gov/pubmed/32041719
http://dx.doi.org/10.1128/AAC.02445-19
Descripción
Sumario:Addition of sodium bicarbonate (NaHCO(3)) to standard antimicrobial susceptibility testing medium reveals certain methicillin-resistant Staphylococcus aureus (MRSA) strains to be highly susceptible to β-lactams. We investigated the prevalence of this phenotype (NaHCO(3) responsiveness) to two β-lactams among 58 clinical MRSA bloodstream isolates. Of note, ∼75% and ∼36% of isolates displayed the NaHCO(3) responsiveness phenotype to cefazolin (CFZ) and oxacillin (OXA), respectively. Neither intrinsic β-lactam MICs in standard Mueller-Hinton broth (MHB) nor population analysis profiles were predictive of this phenotype. Several genotypic markers (clonal complex 8 [CC8]; agr I and spa t008) were associated with NaHCO(3) responsiveness for OXA.

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