Scope and Predictive Genetic/Phenotypic Signatures of Bicarbonate (NaHCO(3)) Responsiveness and β-Lactam Sensitization in Methicillin-Resistant Staphylococcus aureus

Addition of sodium bicarbonate (NaHCO(3)) to standard antimicrobial susceptibility testing medium reveals certain methicillin-resistant Staphylococcus aureus (MRSA) strains to be highly susceptible to β-lactams. We investigated the prevalence of this phenotype (NaHCO(3) responsiveness) to two β-lact...

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Autores principales: Ersoy, Selvi C., Otmishi, Mariam, Milan, Vanessa T., Li, Liang, Pak, Youngju, Mediavilla, Jose, Chen, Liang, Kreiswirth, Barry, Chambers, Henry F., Proctor, Richard A., Xiong, Yan Q., Fowler, Vance G., Bayer, Arnold S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2020
Materias:
Susceptibility
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7179597/
https://www.ncbi.nlm.nih.gov/pubmed/32041719
http://dx.doi.org/10.1128/AAC.02445-19
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author Ersoy, Selvi C.
Otmishi, Mariam
Milan, Vanessa T.
Li, Liang
Pak, Youngju
Mediavilla, Jose
Chen, Liang
Kreiswirth, Barry
Chambers, Henry F.
Proctor, Richard A.
Xiong, Yan Q.
Fowler, Vance G.
Bayer, Arnold S.
author_facet Ersoy, Selvi C.
Otmishi, Mariam
Milan, Vanessa T.
Li, Liang
Pak, Youngju
Mediavilla, Jose
Chen, Liang
Kreiswirth, Barry
Chambers, Henry F.
Proctor, Richard A.
Xiong, Yan Q.
Fowler, Vance G.
Bayer, Arnold S.
author_sort Ersoy, Selvi C.
collection PubMed
description Addition of sodium bicarbonate (NaHCO(3)) to standard antimicrobial susceptibility testing medium reveals certain methicillin-resistant Staphylococcus aureus (MRSA) strains to be highly susceptible to β-lactams. We investigated the prevalence of this phenotype (NaHCO(3) responsiveness) to two β-lactams among 58 clinical MRSA bloodstream isolates. Of note, ∼75% and ∼36% of isolates displayed the NaHCO(3) responsiveness phenotype to cefazolin (CFZ) and oxacillin (OXA), respectively. Neither intrinsic β-lactam MICs in standard Mueller-Hinton broth (MHB) nor population analysis profiles were predictive of this phenotype. Several genotypic markers (clonal complex 8 [CC8]; agr I and spa t008) were associated with NaHCO(3) responsiveness for OXA.
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spelling pubmed-71795972020-04-27 Scope and Predictive Genetic/Phenotypic Signatures of Bicarbonate (NaHCO(3)) Responsiveness and β-Lactam Sensitization in Methicillin-Resistant Staphylococcus aureus Ersoy, Selvi C. Otmishi, Mariam Milan, Vanessa T. Li, Liang Pak, Youngju Mediavilla, Jose Chen, Liang Kreiswirth, Barry Chambers, Henry F. Proctor, Richard A. Xiong, Yan Q. Fowler, Vance G. Bayer, Arnold S. Antimicrob Agents Chemother Susceptibility Addition of sodium bicarbonate (NaHCO(3)) to standard antimicrobial susceptibility testing medium reveals certain methicillin-resistant Staphylococcus aureus (MRSA) strains to be highly susceptible to β-lactams. We investigated the prevalence of this phenotype (NaHCO(3) responsiveness) to two β-lactams among 58 clinical MRSA bloodstream isolates. Of note, ∼75% and ∼36% of isolates displayed the NaHCO(3) responsiveness phenotype to cefazolin (CFZ) and oxacillin (OXA), respectively. Neither intrinsic β-lactam MICs in standard Mueller-Hinton broth (MHB) nor population analysis profiles were predictive of this phenotype. Several genotypic markers (clonal complex 8 [CC8]; agr I and spa t008) were associated with NaHCO(3) responsiveness for OXA. American Society for Microbiology 2020-04-21 /pmc/articles/PMC7179597/ /pubmed/32041719 http://dx.doi.org/10.1128/AAC.02445-19 Text en Copyright © 2020 Ersoy et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Susceptibility
Ersoy, Selvi C.
Otmishi, Mariam
Milan, Vanessa T.
Li, Liang
Pak, Youngju
Mediavilla, Jose
Chen, Liang
Kreiswirth, Barry
Chambers, Henry F.
Proctor, Richard A.
Xiong, Yan Q.
Fowler, Vance G.
Bayer, Arnold S.
Scope and Predictive Genetic/Phenotypic Signatures of Bicarbonate (NaHCO(3)) Responsiveness and β-Lactam Sensitization in Methicillin-Resistant Staphylococcus aureus
title Scope and Predictive Genetic/Phenotypic Signatures of Bicarbonate (NaHCO(3)) Responsiveness and β-Lactam Sensitization in Methicillin-Resistant Staphylococcus aureus
title_full Scope and Predictive Genetic/Phenotypic Signatures of Bicarbonate (NaHCO(3)) Responsiveness and β-Lactam Sensitization in Methicillin-Resistant Staphylococcus aureus
title_fullStr Scope and Predictive Genetic/Phenotypic Signatures of Bicarbonate (NaHCO(3)) Responsiveness and β-Lactam Sensitization in Methicillin-Resistant Staphylococcus aureus
title_full_unstemmed Scope and Predictive Genetic/Phenotypic Signatures of Bicarbonate (NaHCO(3)) Responsiveness and β-Lactam Sensitization in Methicillin-Resistant Staphylococcus aureus
title_short Scope and Predictive Genetic/Phenotypic Signatures of Bicarbonate (NaHCO(3)) Responsiveness and β-Lactam Sensitization in Methicillin-Resistant Staphylococcus aureus
title_sort scope and predictive genetic/phenotypic signatures of bicarbonate (nahco(3)) responsiveness and β-lactam sensitization in methicillin-resistant staphylococcus aureus
topic Susceptibility
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7179597/
https://www.ncbi.nlm.nih.gov/pubmed/32041719
http://dx.doi.org/10.1128/AAC.02445-19
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