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Cellular Analysis and Comparative Transcriptomics Reveal the Tolerance Mechanisms of Candida tropicalis Toward Phenol
Phenol is a ubiquitous pollutant and can contaminate natural water resources. Hence, the removal of phenol from wastewater is of significant importance. A series of biological methods were used to remove phenol based on the natural ability of microorganisms to degrade phenol, but the tolerance mecha...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7179700/ https://www.ncbi.nlm.nih.gov/pubmed/32373081 http://dx.doi.org/10.3389/fmicb.2020.00544 |
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author | Wang, Hanyu Li, Qian Peng, Yuanyuan Zhang, Zhengyue Kuang, Xiaolin Hu, Xiangdong Ayepa, Ellen Han, Xuebing Abrha, Getachew Tafere Xiang, Quanju Yu, Xiumei Zhao, Ke Zou, Likou Gu, Yunfu Li, Xi Li, Xiaoying Chen, Qiang Zhang, Xiaoping Liu, Beidong Ma, Menggen |
author_facet | Wang, Hanyu Li, Qian Peng, Yuanyuan Zhang, Zhengyue Kuang, Xiaolin Hu, Xiangdong Ayepa, Ellen Han, Xuebing Abrha, Getachew Tafere Xiang, Quanju Yu, Xiumei Zhao, Ke Zou, Likou Gu, Yunfu Li, Xi Li, Xiaoying Chen, Qiang Zhang, Xiaoping Liu, Beidong Ma, Menggen |
author_sort | Wang, Hanyu |
collection | PubMed |
description | Phenol is a ubiquitous pollutant and can contaminate natural water resources. Hence, the removal of phenol from wastewater is of significant importance. A series of biological methods were used to remove phenol based on the natural ability of microorganisms to degrade phenol, but the tolerance mechanism of phenol-degraded strains to phenol are not very clear. Morphological observation on Candida tropicalis showed that phenol caused the reactive oxygen species (ROS) accumulation, damaging the mitochondrial and the endoplasmic reticulum. On the basis of transcriptome data and cell wall susceptibility analysis, it was found that C. tropicalis prevented phenol-caused cell damage through improvement of cell wall resistance, maintenance of high-fidelity DNA replication, intracellular protein homeostasis, organelle integrity, and kept the intracellular phenol concentration at a low level through cell-wall remodeling and removal of excess phenol via MDR/MXR transporters. The knowledge obtained will promote the genetic modification of yeast strains in general to tolerate the high concentrations of phenol and improve their efficiency of phenol degradation. |
format | Online Article Text |
id | pubmed-7179700 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71797002020-05-05 Cellular Analysis and Comparative Transcriptomics Reveal the Tolerance Mechanisms of Candida tropicalis Toward Phenol Wang, Hanyu Li, Qian Peng, Yuanyuan Zhang, Zhengyue Kuang, Xiaolin Hu, Xiangdong Ayepa, Ellen Han, Xuebing Abrha, Getachew Tafere Xiang, Quanju Yu, Xiumei Zhao, Ke Zou, Likou Gu, Yunfu Li, Xi Li, Xiaoying Chen, Qiang Zhang, Xiaoping Liu, Beidong Ma, Menggen Front Microbiol Microbiology Phenol is a ubiquitous pollutant and can contaminate natural water resources. Hence, the removal of phenol from wastewater is of significant importance. A series of biological methods were used to remove phenol based on the natural ability of microorganisms to degrade phenol, but the tolerance mechanism of phenol-degraded strains to phenol are not very clear. Morphological observation on Candida tropicalis showed that phenol caused the reactive oxygen species (ROS) accumulation, damaging the mitochondrial and the endoplasmic reticulum. On the basis of transcriptome data and cell wall susceptibility analysis, it was found that C. tropicalis prevented phenol-caused cell damage through improvement of cell wall resistance, maintenance of high-fidelity DNA replication, intracellular protein homeostasis, organelle integrity, and kept the intracellular phenol concentration at a low level through cell-wall remodeling and removal of excess phenol via MDR/MXR transporters. The knowledge obtained will promote the genetic modification of yeast strains in general to tolerate the high concentrations of phenol and improve their efficiency of phenol degradation. Frontiers Media S.A. 2020-04-15 /pmc/articles/PMC7179700/ /pubmed/32373081 http://dx.doi.org/10.3389/fmicb.2020.00544 Text en Copyright © 2020 Wang, Li, Peng, Zhang, Kuang, Hu, Ayepa, Han, Abrha, Xiang, Yu, Zhao, Zou, Gu, Li, Li, Chen, Zhang, Liu and Ma. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Wang, Hanyu Li, Qian Peng, Yuanyuan Zhang, Zhengyue Kuang, Xiaolin Hu, Xiangdong Ayepa, Ellen Han, Xuebing Abrha, Getachew Tafere Xiang, Quanju Yu, Xiumei Zhao, Ke Zou, Likou Gu, Yunfu Li, Xi Li, Xiaoying Chen, Qiang Zhang, Xiaoping Liu, Beidong Ma, Menggen Cellular Analysis and Comparative Transcriptomics Reveal the Tolerance Mechanisms of Candida tropicalis Toward Phenol |
title | Cellular Analysis and Comparative Transcriptomics Reveal the Tolerance Mechanisms of Candida tropicalis Toward Phenol |
title_full | Cellular Analysis and Comparative Transcriptomics Reveal the Tolerance Mechanisms of Candida tropicalis Toward Phenol |
title_fullStr | Cellular Analysis and Comparative Transcriptomics Reveal the Tolerance Mechanisms of Candida tropicalis Toward Phenol |
title_full_unstemmed | Cellular Analysis and Comparative Transcriptomics Reveal the Tolerance Mechanisms of Candida tropicalis Toward Phenol |
title_short | Cellular Analysis and Comparative Transcriptomics Reveal the Tolerance Mechanisms of Candida tropicalis Toward Phenol |
title_sort | cellular analysis and comparative transcriptomics reveal the tolerance mechanisms of candida tropicalis toward phenol |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7179700/ https://www.ncbi.nlm.nih.gov/pubmed/32373081 http://dx.doi.org/10.3389/fmicb.2020.00544 |
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