DNAzyme-Based Target-Triggered Rolling-Circle Amplification for High Sensitivity Detection of microRNAs

MicroRNAs regulate and control the growth and development of cells and can play the role of oncogenes and tumor suppressor genes, which are involved in the occurrence and development of cancers. In this study, DNA fragments obtained by target-induced rolling-circle amplification were constructed to...

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Detalles Bibliográficos
Autores principales: Liu, Chen, Han, Jialun, Zhou, Lujian, Zhang, Jingjing, Du, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7180602/
https://www.ncbi.nlm.nih.gov/pubmed/32260285
http://dx.doi.org/10.3390/s20072017
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author Liu, Chen
Han, Jialun
Zhou, Lujian
Zhang, Jingjing
Du, Jie
author_facet Liu, Chen
Han, Jialun
Zhou, Lujian
Zhang, Jingjing
Du, Jie
author_sort Liu, Chen
collection PubMed
description MicroRNAs regulate and control the growth and development of cells and can play the role of oncogenes and tumor suppressor genes, which are involved in the occurrence and development of cancers. In this study, DNA fragments obtained by target-induced rolling-circle amplification were constructed to complement with self-cleaving deoxyribozyme (DNAzyme) and release fluorescence biomolecules. This sensing approach can affect multiple signal amplification permitting fluorescence detection of microRNAs at the pmol L(−1) level hence affording a simple, highly sensitive, and selective low cost detection platform.
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spelling pubmed-71806022020-05-01 DNAzyme-Based Target-Triggered Rolling-Circle Amplification for High Sensitivity Detection of microRNAs Liu, Chen Han, Jialun Zhou, Lujian Zhang, Jingjing Du, Jie Sensors (Basel) Article MicroRNAs regulate and control the growth and development of cells and can play the role of oncogenes and tumor suppressor genes, which are involved in the occurrence and development of cancers. In this study, DNA fragments obtained by target-induced rolling-circle amplification were constructed to complement with self-cleaving deoxyribozyme (DNAzyme) and release fluorescence biomolecules. This sensing approach can affect multiple signal amplification permitting fluorescence detection of microRNAs at the pmol L(−1) level hence affording a simple, highly sensitive, and selective low cost detection platform. MDPI 2020-04-03 /pmc/articles/PMC7180602/ /pubmed/32260285 http://dx.doi.org/10.3390/s20072017 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Chen
Han, Jialun
Zhou, Lujian
Zhang, Jingjing
Du, Jie
DNAzyme-Based Target-Triggered Rolling-Circle Amplification for High Sensitivity Detection of microRNAs
title DNAzyme-Based Target-Triggered Rolling-Circle Amplification for High Sensitivity Detection of microRNAs
title_full DNAzyme-Based Target-Triggered Rolling-Circle Amplification for High Sensitivity Detection of microRNAs
title_fullStr DNAzyme-Based Target-Triggered Rolling-Circle Amplification for High Sensitivity Detection of microRNAs
title_full_unstemmed DNAzyme-Based Target-Triggered Rolling-Circle Amplification for High Sensitivity Detection of microRNAs
title_short DNAzyme-Based Target-Triggered Rolling-Circle Amplification for High Sensitivity Detection of microRNAs
title_sort dnazyme-based target-triggered rolling-circle amplification for high sensitivity detection of micrornas
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7180602/
https://www.ncbi.nlm.nih.gov/pubmed/32260285
http://dx.doi.org/10.3390/s20072017
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