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Fluorimetric Method for the Determination of Histidine in Random Human Urine Based on Zone Fluidics
In the present study, the determination of histidine (HIS) by an on-line flow method based on the concept of zone fluidics is reported. HIS reacts fast with o-phthalaldehyde at a mildly basic medium (pH 7.5) and in the absence of additional nucleophilic compounds to yield a highly fluorescent deriva...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7180633/ https://www.ncbi.nlm.nih.gov/pubmed/32260350 http://dx.doi.org/10.3390/molecules25071665 |
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author | Alevridis, Antonios Tsiasioti, Apostolia Zacharis, Constantinos K. Tzanavaras, Paraskevas D. |
author_facet | Alevridis, Antonios Tsiasioti, Apostolia Zacharis, Constantinos K. Tzanavaras, Paraskevas D. |
author_sort | Alevridis, Antonios |
collection | PubMed |
description | In the present study, the determination of histidine (HIS) by an on-line flow method based on the concept of zone fluidics is reported. HIS reacts fast with o-phthalaldehyde at a mildly basic medium (pH 7.5) and in the absence of additional nucleophilic compounds to yield a highly fluorescent derivative (λ(ex)/λ(em) = 360/440 nm). The flow procedure was optimized and validated, paying special attention to its selectivity and sensitivity. The LOD was 31 nmol·L(−1), while the within-day and day-to-day precisions were better than 1.0% and 5.0%, respectively (n = 6). Random urine samples from adult volunteers (n = 7) were successfully analyzed without matrix effect (<1%). Endogenous HIS content ranged between 116 and 1527 μmol·L(−1) with percentage recoveries in the range of 87.6%–95.4%. |
format | Online Article Text |
id | pubmed-7180633 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71806332020-05-01 Fluorimetric Method for the Determination of Histidine in Random Human Urine Based on Zone Fluidics Alevridis, Antonios Tsiasioti, Apostolia Zacharis, Constantinos K. Tzanavaras, Paraskevas D. Molecules Article In the present study, the determination of histidine (HIS) by an on-line flow method based on the concept of zone fluidics is reported. HIS reacts fast with o-phthalaldehyde at a mildly basic medium (pH 7.5) and in the absence of additional nucleophilic compounds to yield a highly fluorescent derivative (λ(ex)/λ(em) = 360/440 nm). The flow procedure was optimized and validated, paying special attention to its selectivity and sensitivity. The LOD was 31 nmol·L(−1), while the within-day and day-to-day precisions were better than 1.0% and 5.0%, respectively (n = 6). Random urine samples from adult volunteers (n = 7) were successfully analyzed without matrix effect (<1%). Endogenous HIS content ranged between 116 and 1527 μmol·L(−1) with percentage recoveries in the range of 87.6%–95.4%. MDPI 2020-04-04 /pmc/articles/PMC7180633/ /pubmed/32260350 http://dx.doi.org/10.3390/molecules25071665 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Alevridis, Antonios Tsiasioti, Apostolia Zacharis, Constantinos K. Tzanavaras, Paraskevas D. Fluorimetric Method for the Determination of Histidine in Random Human Urine Based on Zone Fluidics |
title | Fluorimetric Method for the Determination of Histidine in Random Human Urine Based on Zone Fluidics |
title_full | Fluorimetric Method for the Determination of Histidine in Random Human Urine Based on Zone Fluidics |
title_fullStr | Fluorimetric Method for the Determination of Histidine in Random Human Urine Based on Zone Fluidics |
title_full_unstemmed | Fluorimetric Method for the Determination of Histidine in Random Human Urine Based on Zone Fluidics |
title_short | Fluorimetric Method for the Determination of Histidine in Random Human Urine Based on Zone Fluidics |
title_sort | fluorimetric method for the determination of histidine in random human urine based on zone fluidics |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7180633/ https://www.ncbi.nlm.nih.gov/pubmed/32260350 http://dx.doi.org/10.3390/molecules25071665 |
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