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Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing
A fiber optic array (FOA) can be used as an alternative or a supplement to the lens in a microscope due to its large magnification, high coupling efficiency and extremely low distortion. Based on our previous research, this paper first demonstrated the resolution and field-of-view (FOV) of the micro...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7180804/ https://www.ncbi.nlm.nih.gov/pubmed/32276368 http://dx.doi.org/10.3390/s20072092 |
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author | Wang, Weiming Liu, Hang Yu, Yan Cong, Fengyu Yu, Jun |
author_facet | Wang, Weiming Liu, Hang Yu, Yan Cong, Fengyu Yu, Jun |
author_sort | Wang, Weiming |
collection | PubMed |
description | A fiber optic array (FOA) can be used as an alternative or a supplement to the lens in a microscope due to its large magnification, high coupling efficiency and extremely low distortion. Based on our previous research, this paper first demonstrated the resolution and field-of-view (FOV) of the microscope based on the FOA. To further validate the FOA microscope’s imaging capability, yeast activity and concentration were investigated by simple image processing. The results showed that the percentages of live and dead yeast cells correctly identified were 92.1% and 84.8%, except for the clusters, which agreed well with the manual counting methods. Then, the performances of the portable microscopes based on the FOA and lens were compared and the factors that affect the FOA microscope imaging performance were analyzed. |
format | Online Article Text |
id | pubmed-7180804 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71808042020-05-01 Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing Wang, Weiming Liu, Hang Yu, Yan Cong, Fengyu Yu, Jun Sensors (Basel) Article A fiber optic array (FOA) can be used as an alternative or a supplement to the lens in a microscope due to its large magnification, high coupling efficiency and extremely low distortion. Based on our previous research, this paper first demonstrated the resolution and field-of-view (FOV) of the microscope based on the FOA. To further validate the FOA microscope’s imaging capability, yeast activity and concentration were investigated by simple image processing. The results showed that the percentages of live and dead yeast cells correctly identified were 92.1% and 84.8%, except for the clusters, which agreed well with the manual counting methods. Then, the performances of the portable microscopes based on the FOA and lens were compared and the factors that affect the FOA microscope imaging performance were analyzed. MDPI 2020-04-08 /pmc/articles/PMC7180804/ /pubmed/32276368 http://dx.doi.org/10.3390/s20072092 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Weiming Liu, Hang Yu, Yan Cong, Fengyu Yu, Jun Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing |
title | Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing |
title_full | Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing |
title_fullStr | Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing |
title_full_unstemmed | Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing |
title_short | Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing |
title_sort | rapid yeast cell viability analysis by using a portable microscope based on the fiber optic array and simple image processing |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7180804/ https://www.ncbi.nlm.nih.gov/pubmed/32276368 http://dx.doi.org/10.3390/s20072092 |
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