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Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing

A fiber optic array (FOA) can be used as an alternative or a supplement to the lens in a microscope due to its large magnification, high coupling efficiency and extremely low distortion. Based on our previous research, this paper first demonstrated the resolution and field-of-view (FOV) of the micro...

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Detalles Bibliográficos
Autores principales: Wang, Weiming, Liu, Hang, Yu, Yan, Cong, Fengyu, Yu, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7180804/
https://www.ncbi.nlm.nih.gov/pubmed/32276368
http://dx.doi.org/10.3390/s20072092
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author Wang, Weiming
Liu, Hang
Yu, Yan
Cong, Fengyu
Yu, Jun
author_facet Wang, Weiming
Liu, Hang
Yu, Yan
Cong, Fengyu
Yu, Jun
author_sort Wang, Weiming
collection PubMed
description A fiber optic array (FOA) can be used as an alternative or a supplement to the lens in a microscope due to its large magnification, high coupling efficiency and extremely low distortion. Based on our previous research, this paper first demonstrated the resolution and field-of-view (FOV) of the microscope based on the FOA. To further validate the FOA microscope’s imaging capability, yeast activity and concentration were investigated by simple image processing. The results showed that the percentages of live and dead yeast cells correctly identified were 92.1% and 84.8%, except for the clusters, which agreed well with the manual counting methods. Then, the performances of the portable microscopes based on the FOA and lens were compared and the factors that affect the FOA microscope imaging performance were analyzed.
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spelling pubmed-71808042020-05-01 Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing Wang, Weiming Liu, Hang Yu, Yan Cong, Fengyu Yu, Jun Sensors (Basel) Article A fiber optic array (FOA) can be used as an alternative or a supplement to the lens in a microscope due to its large magnification, high coupling efficiency and extremely low distortion. Based on our previous research, this paper first demonstrated the resolution and field-of-view (FOV) of the microscope based on the FOA. To further validate the FOA microscope’s imaging capability, yeast activity and concentration were investigated by simple image processing. The results showed that the percentages of live and dead yeast cells correctly identified were 92.1% and 84.8%, except for the clusters, which agreed well with the manual counting methods. Then, the performances of the portable microscopes based on the FOA and lens were compared and the factors that affect the FOA microscope imaging performance were analyzed. MDPI 2020-04-08 /pmc/articles/PMC7180804/ /pubmed/32276368 http://dx.doi.org/10.3390/s20072092 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Weiming
Liu, Hang
Yu, Yan
Cong, Fengyu
Yu, Jun
Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing
title Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing
title_full Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing
title_fullStr Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing
title_full_unstemmed Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing
title_short Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image Processing
title_sort rapid yeast cell viability analysis by using a portable microscope based on the fiber optic array and simple image processing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7180804/
https://www.ncbi.nlm.nih.gov/pubmed/32276368
http://dx.doi.org/10.3390/s20072092
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