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Purification, Identification, and Characterization of an Endo-1,4-β-Xylanase from Wheat Malt

In this study, an endo-1,4-β-xylanase was purified from wheat malt following the procedures of ammonium sulfate precipitation, cation-exchange chromatography, and two-step anion-exchange chromatography. The purified endo-1,4-β-xylanase had a specific activity of 3.94 u/mg, demonstrating a weight ave...

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Autores principales: Peng, Zhaojun, Jin, Yuhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7180899/
https://www.ncbi.nlm.nih.gov/pubmed/32235408
http://dx.doi.org/10.3390/molecules25071572
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author Peng, Zhaojun
Jin, Yuhong
author_facet Peng, Zhaojun
Jin, Yuhong
author_sort Peng, Zhaojun
collection PubMed
description In this study, an endo-1,4-β-xylanase was purified from wheat malt following the procedures of ammonium sulfate precipitation, cation-exchange chromatography, and two-step anion-exchange chromatography. The purified endo-1,4-β-xylanase had a specific activity of 3.94 u/mg, demonstrating a weight average molecular weight (Mw) of approximately 58,000 Da. After LC–MS/MS (Liquid chromatography-tandem mass spectrometry) identification, the purified enzyme had the highest matching degree with a GH10 (Glycoside Hydrolase 10) domain-containing protein from wheat, there were 23 match peptides with a score above the threshold and the prot-cover was 45.5%. The resulting purified enzyme was used to investigate its degradation ability on high viscosity wheat-derived water-extractable arabinoxylan (WEAX). Degradation experiments confirmed that the purified enzyme was a true endo-acting enzyme, which could degrade large WEAX into smaller WEAX. The average degree of polymerization (avDP) and the viscosity of WEAX decreased with the increasing reaction time. The enzyme could degrade a small amount of WEAX into arabinoxylan-oligosaccharides (AXOS) with a degree of polymerization of 2–6, but no monosaccharide was produced. The degradation occurred rapidly in the first 3.5 h and decreased with the further prolongation of reaction time.
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spelling pubmed-71808992020-05-01 Purification, Identification, and Characterization of an Endo-1,4-β-Xylanase from Wheat Malt Peng, Zhaojun Jin, Yuhong Molecules Article In this study, an endo-1,4-β-xylanase was purified from wheat malt following the procedures of ammonium sulfate precipitation, cation-exchange chromatography, and two-step anion-exchange chromatography. The purified endo-1,4-β-xylanase had a specific activity of 3.94 u/mg, demonstrating a weight average molecular weight (Mw) of approximately 58,000 Da. After LC–MS/MS (Liquid chromatography-tandem mass spectrometry) identification, the purified enzyme had the highest matching degree with a GH10 (Glycoside Hydrolase 10) domain-containing protein from wheat, there were 23 match peptides with a score above the threshold and the prot-cover was 45.5%. The resulting purified enzyme was used to investigate its degradation ability on high viscosity wheat-derived water-extractable arabinoxylan (WEAX). Degradation experiments confirmed that the purified enzyme was a true endo-acting enzyme, which could degrade large WEAX into smaller WEAX. The average degree of polymerization (avDP) and the viscosity of WEAX decreased with the increasing reaction time. The enzyme could degrade a small amount of WEAX into arabinoxylan-oligosaccharides (AXOS) with a degree of polymerization of 2–6, but no monosaccharide was produced. The degradation occurred rapidly in the first 3.5 h and decreased with the further prolongation of reaction time. MDPI 2020-03-29 /pmc/articles/PMC7180899/ /pubmed/32235408 http://dx.doi.org/10.3390/molecules25071572 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Peng, Zhaojun
Jin, Yuhong
Purification, Identification, and Characterization of an Endo-1,4-β-Xylanase from Wheat Malt
title Purification, Identification, and Characterization of an Endo-1,4-β-Xylanase from Wheat Malt
title_full Purification, Identification, and Characterization of an Endo-1,4-β-Xylanase from Wheat Malt
title_fullStr Purification, Identification, and Characterization of an Endo-1,4-β-Xylanase from Wheat Malt
title_full_unstemmed Purification, Identification, and Characterization of an Endo-1,4-β-Xylanase from Wheat Malt
title_short Purification, Identification, and Characterization of an Endo-1,4-β-Xylanase from Wheat Malt
title_sort purification, identification, and characterization of an endo-1,4-β-xylanase from wheat malt
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7180899/
https://www.ncbi.nlm.nih.gov/pubmed/32235408
http://dx.doi.org/10.3390/molecules25071572
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