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Circulating CD14(+)HLA-DR(−/low) Myeloid-Derived Suppressor Cells as Potential Biomarkers for the Identification of Psoriasis TCM Blood-Heat Syndrome and Blood-Stasis Syndrome

Psoriasis is a chronic autoimmune disease. Identification of the biomarkers responsible for Traditional Chinese Medicine (TCM) syndromes of psoriasis can help researchers recognize the different aspects of psoriasis and find novel therapeutic targets for the treatment of psoriasis. The current study...

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Autores principales: Sun, Shipeng, Wei, Yali, Zeng, Xue, Yuan, Yuliang, Wang, Na, An, Cheng, Duan, Jinlong, Pang, Bo, Hong, Zifu, Liu, Guijian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7180989/
https://www.ncbi.nlm.nih.gov/pubmed/32382290
http://dx.doi.org/10.1155/2020/4582459
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author Sun, Shipeng
Wei, Yali
Zeng, Xue
Yuan, Yuliang
Wang, Na
An, Cheng
Duan, Jinlong
Pang, Bo
Hong, Zifu
Liu, Guijian
author_facet Sun, Shipeng
Wei, Yali
Zeng, Xue
Yuan, Yuliang
Wang, Na
An, Cheng
Duan, Jinlong
Pang, Bo
Hong, Zifu
Liu, Guijian
author_sort Sun, Shipeng
collection PubMed
description Psoriasis is a chronic autoimmune disease. Identification of the biomarkers responsible for Traditional Chinese Medicine (TCM) syndromes of psoriasis can help researchers recognize the different aspects of psoriasis and find novel therapeutic targets for the treatment of psoriasis. The current study investigated the levels of circulating Mo-MDSCs and Mo-MDSC-associated immune factors in the peripheral blood of psoriasis patients with different TCM syndromes. We found that the frequency of Mo-MDSCs (CD14(+)HLA-DR(−/low) cells) among CD14(+) cells from plaque psoriasis patients with blood-stasis (BS) syndrome was significantly increased when compared with healthy controls (p < 0.001) and blood-heat (BH) syndrome group (p < 0.001), respectively. However, serum IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-α, IFN-γ, iNOS, Arg-1, and NO concentration showed no statistically significant difference between healthy controls and psoriasis patients as well as no significant difference between the BH and BS syndrome groups. Compared with healthy controls, the mRNA expression of Arg-1, TNF-α, ROR-γ, and PD-L1 was increased, while the mRNA expression of PD-1 and IL-10 was decreased in PBMCs from psoriasis patients. Moreover, the mRNA expression of TNF-α and FOXP3 in PBMCs showed a pronounced statistical difference between the psoriatic BH syndrome group and the BS syndrome group. Therefore, we provide evidence that the percentage of CD14(+)HLA-DR(−/low) MDSC/ CD14(+) cells and TNF-α and Foxp3 mRNA expression levels in PBMCs are potential biomarkers for distinguishing TCM BH syndrome and BS syndrome.
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spelling pubmed-71809892020-05-07 Circulating CD14(+)HLA-DR(−/low) Myeloid-Derived Suppressor Cells as Potential Biomarkers for the Identification of Psoriasis TCM Blood-Heat Syndrome and Blood-Stasis Syndrome Sun, Shipeng Wei, Yali Zeng, Xue Yuan, Yuliang Wang, Na An, Cheng Duan, Jinlong Pang, Bo Hong, Zifu Liu, Guijian Evid Based Complement Alternat Med Research Article Psoriasis is a chronic autoimmune disease. Identification of the biomarkers responsible for Traditional Chinese Medicine (TCM) syndromes of psoriasis can help researchers recognize the different aspects of psoriasis and find novel therapeutic targets for the treatment of psoriasis. The current study investigated the levels of circulating Mo-MDSCs and Mo-MDSC-associated immune factors in the peripheral blood of psoriasis patients with different TCM syndromes. We found that the frequency of Mo-MDSCs (CD14(+)HLA-DR(−/low) cells) among CD14(+) cells from plaque psoriasis patients with blood-stasis (BS) syndrome was significantly increased when compared with healthy controls (p < 0.001) and blood-heat (BH) syndrome group (p < 0.001), respectively. However, serum IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-α, IFN-γ, iNOS, Arg-1, and NO concentration showed no statistically significant difference between healthy controls and psoriasis patients as well as no significant difference between the BH and BS syndrome groups. Compared with healthy controls, the mRNA expression of Arg-1, TNF-α, ROR-γ, and PD-L1 was increased, while the mRNA expression of PD-1 and IL-10 was decreased in PBMCs from psoriasis patients. Moreover, the mRNA expression of TNF-α and FOXP3 in PBMCs showed a pronounced statistical difference between the psoriatic BH syndrome group and the BS syndrome group. Therefore, we provide evidence that the percentage of CD14(+)HLA-DR(−/low) MDSC/ CD14(+) cells and TNF-α and Foxp3 mRNA expression levels in PBMCs are potential biomarkers for distinguishing TCM BH syndrome and BS syndrome. Hindawi 2020-04-14 /pmc/articles/PMC7180989/ /pubmed/32382290 http://dx.doi.org/10.1155/2020/4582459 Text en Copyright © 2020 Shipeng Sun et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sun, Shipeng
Wei, Yali
Zeng, Xue
Yuan, Yuliang
Wang, Na
An, Cheng
Duan, Jinlong
Pang, Bo
Hong, Zifu
Liu, Guijian
Circulating CD14(+)HLA-DR(−/low) Myeloid-Derived Suppressor Cells as Potential Biomarkers for the Identification of Psoriasis TCM Blood-Heat Syndrome and Blood-Stasis Syndrome
title Circulating CD14(+)HLA-DR(−/low) Myeloid-Derived Suppressor Cells as Potential Biomarkers for the Identification of Psoriasis TCM Blood-Heat Syndrome and Blood-Stasis Syndrome
title_full Circulating CD14(+)HLA-DR(−/low) Myeloid-Derived Suppressor Cells as Potential Biomarkers for the Identification of Psoriasis TCM Blood-Heat Syndrome and Blood-Stasis Syndrome
title_fullStr Circulating CD14(+)HLA-DR(−/low) Myeloid-Derived Suppressor Cells as Potential Biomarkers for the Identification of Psoriasis TCM Blood-Heat Syndrome and Blood-Stasis Syndrome
title_full_unstemmed Circulating CD14(+)HLA-DR(−/low) Myeloid-Derived Suppressor Cells as Potential Biomarkers for the Identification of Psoriasis TCM Blood-Heat Syndrome and Blood-Stasis Syndrome
title_short Circulating CD14(+)HLA-DR(−/low) Myeloid-Derived Suppressor Cells as Potential Biomarkers for the Identification of Psoriasis TCM Blood-Heat Syndrome and Blood-Stasis Syndrome
title_sort circulating cd14(+)hla-dr(−/low) myeloid-derived suppressor cells as potential biomarkers for the identification of psoriasis tcm blood-heat syndrome and blood-stasis syndrome
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7180989/
https://www.ncbi.nlm.nih.gov/pubmed/32382290
http://dx.doi.org/10.1155/2020/4582459
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