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Himalayan Nettle Girardinia diversifolia as a Candidate Ingredient for Pharmaceutical and Nutraceutical Applications—Phytochemical Analysis and In Vitro Bioassays

Girardinia diversifolia, also known as Himalayan nettle, is a perennial herb used in Nepal to make fiber as well as in traditional medicine for the treatment of several diseases. To date, phytochemical studies and biological assays on this plant are scarce. Thus, in the present work, the G. diversif...

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Autores principales: Sharan Shrestha, Shyam, Sut, Stefania, Ferrarese, Irene, Barbon Di Marco, Serena, Zengin, Gokhan, De Franco, Michele, Pant, Deepak Raj, Mahomoodally, Mohamad Fawzi, Ferri, Nicola, Biancorosso, Noemi, Maggi, Filippo, Dall Acqua, Stefano, Rajbhandary, Sangeeta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7180999/
https://www.ncbi.nlm.nih.gov/pubmed/32235298
http://dx.doi.org/10.3390/molecules25071563
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author Sharan Shrestha, Shyam
Sut, Stefania
Ferrarese, Irene
Barbon Di Marco, Serena
Zengin, Gokhan
De Franco, Michele
Pant, Deepak Raj
Mahomoodally, Mohamad Fawzi
Ferri, Nicola
Biancorosso, Noemi
Maggi, Filippo
Dall Acqua, Stefano
Rajbhandary, Sangeeta
author_facet Sharan Shrestha, Shyam
Sut, Stefania
Ferrarese, Irene
Barbon Di Marco, Serena
Zengin, Gokhan
De Franco, Michele
Pant, Deepak Raj
Mahomoodally, Mohamad Fawzi
Ferri, Nicola
Biancorosso, Noemi
Maggi, Filippo
Dall Acqua, Stefano
Rajbhandary, Sangeeta
author_sort Sharan Shrestha, Shyam
collection PubMed
description Girardinia diversifolia, also known as Himalayan nettle, is a perennial herb used in Nepal to make fiber as well as in traditional medicine for the treatment of several diseases. To date, phytochemical studies and biological assays on this plant are scarce. Thus, in the present work, the G. diversifolia extracts have been evaluated for their potential pharmaceutical, cosmetic and nutraceutical uses. For this purpose, detailed phytochemical analyses were performed, evidencing the presence of phytosterols, fatty acids, carotenoids, polyphenols and saponins. The most abundant secondary metabolites were β- and γ-sitosterol (11 and 9% dw, respectively), and trans syringin (0.5 mg/g) was the most abundant phenolic. Fatty acids with an abundant portion of unsaturated derivatives (linoleic and linolenic acid at 22.0 and 9.7 mg/g respectively), vitamin C (2.9 mg/g) and vitamin B2 (0.12 mg/g) were also present. The antioxidant activity was moderate while a significant ability to inhibit acetylcholinesterase (AChE), butyrilcholinesterase (BuChE), tyrosinase, α-amylase and α-glucosidase was observed. A cytotoxic effect was observed on human ovarian, pancreatic and hepatic cancer cell lines. The effect in hepatocarcinoma cells was associated to a downregulation of the low-density lipoprotein receptor (LDLR), a pivotal regulator of cellular cholesterol homeostasis. These data show the potential usefulness of this species for possible applications in pharmaceuticals, nutraceuticals and cosmetics.
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spelling pubmed-71809992020-04-30 Himalayan Nettle Girardinia diversifolia as a Candidate Ingredient for Pharmaceutical and Nutraceutical Applications—Phytochemical Analysis and In Vitro Bioassays Sharan Shrestha, Shyam Sut, Stefania Ferrarese, Irene Barbon Di Marco, Serena Zengin, Gokhan De Franco, Michele Pant, Deepak Raj Mahomoodally, Mohamad Fawzi Ferri, Nicola Biancorosso, Noemi Maggi, Filippo Dall Acqua, Stefano Rajbhandary, Sangeeta Molecules Article Girardinia diversifolia, also known as Himalayan nettle, is a perennial herb used in Nepal to make fiber as well as in traditional medicine for the treatment of several diseases. To date, phytochemical studies and biological assays on this plant are scarce. Thus, in the present work, the G. diversifolia extracts have been evaluated for their potential pharmaceutical, cosmetic and nutraceutical uses. For this purpose, detailed phytochemical analyses were performed, evidencing the presence of phytosterols, fatty acids, carotenoids, polyphenols and saponins. The most abundant secondary metabolites were β- and γ-sitosterol (11 and 9% dw, respectively), and trans syringin (0.5 mg/g) was the most abundant phenolic. Fatty acids with an abundant portion of unsaturated derivatives (linoleic and linolenic acid at 22.0 and 9.7 mg/g respectively), vitamin C (2.9 mg/g) and vitamin B2 (0.12 mg/g) were also present. The antioxidant activity was moderate while a significant ability to inhibit acetylcholinesterase (AChE), butyrilcholinesterase (BuChE), tyrosinase, α-amylase and α-glucosidase was observed. A cytotoxic effect was observed on human ovarian, pancreatic and hepatic cancer cell lines. The effect in hepatocarcinoma cells was associated to a downregulation of the low-density lipoprotein receptor (LDLR), a pivotal regulator of cellular cholesterol homeostasis. These data show the potential usefulness of this species for possible applications in pharmaceuticals, nutraceuticals and cosmetics. MDPI 2020-03-29 /pmc/articles/PMC7180999/ /pubmed/32235298 http://dx.doi.org/10.3390/molecules25071563 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sharan Shrestha, Shyam
Sut, Stefania
Ferrarese, Irene
Barbon Di Marco, Serena
Zengin, Gokhan
De Franco, Michele
Pant, Deepak Raj
Mahomoodally, Mohamad Fawzi
Ferri, Nicola
Biancorosso, Noemi
Maggi, Filippo
Dall Acqua, Stefano
Rajbhandary, Sangeeta
Himalayan Nettle Girardinia diversifolia as a Candidate Ingredient for Pharmaceutical and Nutraceutical Applications—Phytochemical Analysis and In Vitro Bioassays
title Himalayan Nettle Girardinia diversifolia as a Candidate Ingredient for Pharmaceutical and Nutraceutical Applications—Phytochemical Analysis and In Vitro Bioassays
title_full Himalayan Nettle Girardinia diversifolia as a Candidate Ingredient for Pharmaceutical and Nutraceutical Applications—Phytochemical Analysis and In Vitro Bioassays
title_fullStr Himalayan Nettle Girardinia diversifolia as a Candidate Ingredient for Pharmaceutical and Nutraceutical Applications—Phytochemical Analysis and In Vitro Bioassays
title_full_unstemmed Himalayan Nettle Girardinia diversifolia as a Candidate Ingredient for Pharmaceutical and Nutraceutical Applications—Phytochemical Analysis and In Vitro Bioassays
title_short Himalayan Nettle Girardinia diversifolia as a Candidate Ingredient for Pharmaceutical and Nutraceutical Applications—Phytochemical Analysis and In Vitro Bioassays
title_sort himalayan nettle girardinia diversifolia as a candidate ingredient for pharmaceutical and nutraceutical applications—phytochemical analysis and in vitro bioassays
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7180999/
https://www.ncbi.nlm.nih.gov/pubmed/32235298
http://dx.doi.org/10.3390/molecules25071563
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