Cellular Internalization of Beta-Carotene Loaded Polyelectrolyte Multilayer Capsules by Raman Mapping

Raman mapping is becoming a very useful tool in investigating cells and cellular components, as well as bioactive molecules intracellularly. In this study, we have encapsulated beta-carotene using a layer-by-layer technique, as a way to enhance its stability and bioavailability. Further, we have use...

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Autores principales: Leopold, Loredana F., Marișca, Oana, Oprea, Ioana, Rugină, Dumitrița, Suciu, Maria, Nistor, Mădălina, Tofană, Maria, Leopold, Nicolae, Coman, Cristina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181117/
https://www.ncbi.nlm.nih.gov/pubmed/32218110
http://dx.doi.org/10.3390/molecules25071477
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author Leopold, Loredana F.
Marișca, Oana
Oprea, Ioana
Rugină, Dumitrița
Suciu, Maria
Nistor, Mădălina
Tofană, Maria
Leopold, Nicolae
Coman, Cristina
author_facet Leopold, Loredana F.
Marișca, Oana
Oprea, Ioana
Rugină, Dumitrița
Suciu, Maria
Nistor, Mădălina
Tofană, Maria
Leopold, Nicolae
Coman, Cristina
author_sort Leopold, Loredana F.
collection PubMed
description Raman mapping is becoming a very useful tool in investigating cells and cellular components, as well as bioactive molecules intracellularly. In this study, we have encapsulated beta-carotene using a layer-by-layer technique, as a way to enhance its stability and bioavailability. Further, we have used Raman mapping to characterize the as-obtained capsules and monitor their uptake by the human retinal epithelial D407 cells. We were able to successfully map the beta-carotene distribution inside the capsules, to localize the capsules intracellularly, and distinguish between capsules and other cellular components.
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spelling pubmed-71811172020-04-30 Cellular Internalization of Beta-Carotene Loaded Polyelectrolyte Multilayer Capsules by Raman Mapping Leopold, Loredana F. Marișca, Oana Oprea, Ioana Rugină, Dumitrița Suciu, Maria Nistor, Mădălina Tofană, Maria Leopold, Nicolae Coman, Cristina Molecules Article Raman mapping is becoming a very useful tool in investigating cells and cellular components, as well as bioactive molecules intracellularly. In this study, we have encapsulated beta-carotene using a layer-by-layer technique, as a way to enhance its stability and bioavailability. Further, we have used Raman mapping to characterize the as-obtained capsules and monitor their uptake by the human retinal epithelial D407 cells. We were able to successfully map the beta-carotene distribution inside the capsules, to localize the capsules intracellularly, and distinguish between capsules and other cellular components. MDPI 2020-03-25 /pmc/articles/PMC7181117/ /pubmed/32218110 http://dx.doi.org/10.3390/molecules25071477 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Leopold, Loredana F.
Marișca, Oana
Oprea, Ioana
Rugină, Dumitrița
Suciu, Maria
Nistor, Mădălina
Tofană, Maria
Leopold, Nicolae
Coman, Cristina
Cellular Internalization of Beta-Carotene Loaded Polyelectrolyte Multilayer Capsules by Raman Mapping
title Cellular Internalization of Beta-Carotene Loaded Polyelectrolyte Multilayer Capsules by Raman Mapping
title_full Cellular Internalization of Beta-Carotene Loaded Polyelectrolyte Multilayer Capsules by Raman Mapping
title_fullStr Cellular Internalization of Beta-Carotene Loaded Polyelectrolyte Multilayer Capsules by Raman Mapping
title_full_unstemmed Cellular Internalization of Beta-Carotene Loaded Polyelectrolyte Multilayer Capsules by Raman Mapping
title_short Cellular Internalization of Beta-Carotene Loaded Polyelectrolyte Multilayer Capsules by Raman Mapping
title_sort cellular internalization of beta-carotene loaded polyelectrolyte multilayer capsules by raman mapping
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181117/
https://www.ncbi.nlm.nih.gov/pubmed/32218110
http://dx.doi.org/10.3390/molecules25071477
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