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Integrative Proteome and Acetylome Analyses of Murine Responses to Cryptococcus neoformans Infection

Cryptococcus neoformans is a causative agent for pulmonary infection and meningoencephalitis. Understanding the host’s response to C. neoformans infection is critical for developing effective treatment. Even though some have elucidated the host response at the transcriptome level, little is known ab...

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Autores principales: Li, Hailong, Li, Yanjian, Sun, Tianshu, Du, Wei, Zhang, Zhijie, Li, Dancheng, Ding, Chen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181412/
https://www.ncbi.nlm.nih.gov/pubmed/32362878
http://dx.doi.org/10.3389/fmicb.2020.00575
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author Li, Hailong
Li, Yanjian
Sun, Tianshu
Du, Wei
Zhang, Zhijie
Li, Dancheng
Ding, Chen
author_facet Li, Hailong
Li, Yanjian
Sun, Tianshu
Du, Wei
Zhang, Zhijie
Li, Dancheng
Ding, Chen
author_sort Li, Hailong
collection PubMed
description Cryptococcus neoformans is a causative agent for pulmonary infection and meningoencephalitis. Understanding the host’s response to C. neoformans infection is critical for developing effective treatment. Even though some have elucidated the host response at the transcriptome level, little is known about how it modulates its defense machinery through the proteome mechanism or how protein posttranslational modification responds to the infection. In this work, we employed a murine infection model and mass spectrometry to systematically determine the proteome and acetylome statuses of lungs and brains in the early stage of infection. To extensively analyze the host response, we integrated the proteome data to the transcriptome results. Critical genes, including genes involved in phagosome, lysosome, and platelet activation are significantly altered in protein and gene expression during infection. In the acetylome analysis, we demonstrated that lung and brain tissues differentially regulate protein acetylation during infection. The three primary groups of proteins altered in acetylation status are histones, proteins involved in glucose and fatty acid metabolism, and proteins from the immune system. These analyses provide an integrative regulation network of the host responding to C. neoformans and shed new light on understanding the host’s regulation mechanism when responding to C. neoformans.
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spelling pubmed-71814122020-05-01 Integrative Proteome and Acetylome Analyses of Murine Responses to Cryptococcus neoformans Infection Li, Hailong Li, Yanjian Sun, Tianshu Du, Wei Zhang, Zhijie Li, Dancheng Ding, Chen Front Microbiol Microbiology Cryptococcus neoformans is a causative agent for pulmonary infection and meningoencephalitis. Understanding the host’s response to C. neoformans infection is critical for developing effective treatment. Even though some have elucidated the host response at the transcriptome level, little is known about how it modulates its defense machinery through the proteome mechanism or how protein posttranslational modification responds to the infection. In this work, we employed a murine infection model and mass spectrometry to systematically determine the proteome and acetylome statuses of lungs and brains in the early stage of infection. To extensively analyze the host response, we integrated the proteome data to the transcriptome results. Critical genes, including genes involved in phagosome, lysosome, and platelet activation are significantly altered in protein and gene expression during infection. In the acetylome analysis, we demonstrated that lung and brain tissues differentially regulate protein acetylation during infection. The three primary groups of proteins altered in acetylation status are histones, proteins involved in glucose and fatty acid metabolism, and proteins from the immune system. These analyses provide an integrative regulation network of the host responding to C. neoformans and shed new light on understanding the host’s regulation mechanism when responding to C. neoformans. Frontiers Media S.A. 2020-04-17 /pmc/articles/PMC7181412/ /pubmed/32362878 http://dx.doi.org/10.3389/fmicb.2020.00575 Text en Copyright © 2020 Li, Li, Sun, Du, Zhang, Li and Ding. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Li, Hailong
Li, Yanjian
Sun, Tianshu
Du, Wei
Zhang, Zhijie
Li, Dancheng
Ding, Chen
Integrative Proteome and Acetylome Analyses of Murine Responses to Cryptococcus neoformans Infection
title Integrative Proteome and Acetylome Analyses of Murine Responses to Cryptococcus neoformans Infection
title_full Integrative Proteome and Acetylome Analyses of Murine Responses to Cryptococcus neoformans Infection
title_fullStr Integrative Proteome and Acetylome Analyses of Murine Responses to Cryptococcus neoformans Infection
title_full_unstemmed Integrative Proteome and Acetylome Analyses of Murine Responses to Cryptococcus neoformans Infection
title_short Integrative Proteome and Acetylome Analyses of Murine Responses to Cryptococcus neoformans Infection
title_sort integrative proteome and acetylome analyses of murine responses to cryptococcus neoformans infection
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181412/
https://www.ncbi.nlm.nih.gov/pubmed/32362878
http://dx.doi.org/10.3389/fmicb.2020.00575
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