Interleukin-1α associates with the tumor suppressor p53 following DNA damage

Interleukin-1α (IL-1α) is a dual-function proinflammatory mediator. In addition to its role in the canonical IL-1 signaling pathway, which employs membrane-bound receptors, a growing body of evidence shows that IL-1α has some additional intracellular functions. We identified the interaction of IL-1α...

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Autores principales: Novak, J., Zamostna, B., Vopalensky, V., Buryskova, M., Burysek, L., Doleckova, D., Pospisek, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181607/
https://www.ncbi.nlm.nih.gov/pubmed/32332775
http://dx.doi.org/10.1038/s41598-020-63779-x
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author Novak, J.
Zamostna, B.
Vopalensky, V.
Buryskova, M.
Burysek, L.
Doleckova, D.
Pospisek, M.
author_facet Novak, J.
Zamostna, B.
Vopalensky, V.
Buryskova, M.
Burysek, L.
Doleckova, D.
Pospisek, M.
author_sort Novak, J.
collection PubMed
description Interleukin-1α (IL-1α) is a dual-function proinflammatory mediator. In addition to its role in the canonical IL-1 signaling pathway, which employs membrane-bound receptors, a growing body of evidence shows that IL-1α has some additional intracellular functions. We identified the interaction of IL-1α with the tumor suppressor p53 in the nuclei and cytoplasm of both malignant and noncancerous mammalian cell lines using immunoprecipitation and the in situ proximity ligation assay (PLA). This interaction was enhanced by treatment with the antineoplastic drug etoposide, which suggests a role for the IL-1α•p53 interaction in genotoxic stress.
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spelling pubmed-71816072020-04-27 Interleukin-1α associates with the tumor suppressor p53 following DNA damage Novak, J. Zamostna, B. Vopalensky, V. Buryskova, M. Burysek, L. Doleckova, D. Pospisek, M. Sci Rep Article Interleukin-1α (IL-1α) is a dual-function proinflammatory mediator. In addition to its role in the canonical IL-1 signaling pathway, which employs membrane-bound receptors, a growing body of evidence shows that IL-1α has some additional intracellular functions. We identified the interaction of IL-1α with the tumor suppressor p53 in the nuclei and cytoplasm of both malignant and noncancerous mammalian cell lines using immunoprecipitation and the in situ proximity ligation assay (PLA). This interaction was enhanced by treatment with the antineoplastic drug etoposide, which suggests a role for the IL-1α•p53 interaction in genotoxic stress. Nature Publishing Group UK 2020-04-24 /pmc/articles/PMC7181607/ /pubmed/32332775 http://dx.doi.org/10.1038/s41598-020-63779-x Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Novak, J.
Zamostna, B.
Vopalensky, V.
Buryskova, M.
Burysek, L.
Doleckova, D.
Pospisek, M.
Interleukin-1α associates with the tumor suppressor p53 following DNA damage
title Interleukin-1α associates with the tumor suppressor p53 following DNA damage
title_full Interleukin-1α associates with the tumor suppressor p53 following DNA damage
title_fullStr Interleukin-1α associates with the tumor suppressor p53 following DNA damage
title_full_unstemmed Interleukin-1α associates with the tumor suppressor p53 following DNA damage
title_short Interleukin-1α associates with the tumor suppressor p53 following DNA damage
title_sort interleukin-1α associates with the tumor suppressor p53 following dna damage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181607/
https://www.ncbi.nlm.nih.gov/pubmed/32332775
http://dx.doi.org/10.1038/s41598-020-63779-x
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