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Biologically driven cut-off definition of lymphocyte ratios in metastatic breast cancer and association with exosomal subpopulations and prognosis

High neutrophil to lymphocyte ratio (NLR) and monocyte to lymphocyte ratio (MLR) are respectively associated with systemic inflammation and immune suppression and have been associated with a poor outcome. Plasmatic exosomes are extracellular vesicles involved in the intercellular communication syste...

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Autores principales: Gerratana, Lorenzo, Basile, Debora, Toffoletto, Barbara, Bulfoni, Michela, Zago, Silvia, Magini, Alessandro, Lera, Marta, Pelizzari, Giacomo, Parisse, Pietro, Casalis, Loredana, Vitale, Maria Grazia, Fanotto, Valentina, Bonotto, Marta, Caponnetto, Federica, Bartoletti, Michele, Lisanti, Camilla, Minisini, Alessandro Marco, Emiliani, Carla, Di Loreto, Carla, Fasola, Gianpiero, Curcio, Francesco, Beltrami, Antonio Paolo, Cesselli, Daniela, Puglisi, Fabio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181663/
https://www.ncbi.nlm.nih.gov/pubmed/32332763
http://dx.doi.org/10.1038/s41598-020-63291-2
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author Gerratana, Lorenzo
Basile, Debora
Toffoletto, Barbara
Bulfoni, Michela
Zago, Silvia
Magini, Alessandro
Lera, Marta
Pelizzari, Giacomo
Parisse, Pietro
Casalis, Loredana
Vitale, Maria Grazia
Fanotto, Valentina
Bonotto, Marta
Caponnetto, Federica
Bartoletti, Michele
Lisanti, Camilla
Minisini, Alessandro Marco
Emiliani, Carla
Di Loreto, Carla
Fasola, Gianpiero
Curcio, Francesco
Beltrami, Antonio Paolo
Cesselli, Daniela
Puglisi, Fabio
author_facet Gerratana, Lorenzo
Basile, Debora
Toffoletto, Barbara
Bulfoni, Michela
Zago, Silvia
Magini, Alessandro
Lera, Marta
Pelizzari, Giacomo
Parisse, Pietro
Casalis, Loredana
Vitale, Maria Grazia
Fanotto, Valentina
Bonotto, Marta
Caponnetto, Federica
Bartoletti, Michele
Lisanti, Camilla
Minisini, Alessandro Marco
Emiliani, Carla
Di Loreto, Carla
Fasola, Gianpiero
Curcio, Francesco
Beltrami, Antonio Paolo
Cesselli, Daniela
Puglisi, Fabio
author_sort Gerratana, Lorenzo
collection PubMed
description High neutrophil to lymphocyte ratio (NLR) and monocyte to lymphocyte ratio (MLR) are respectively associated with systemic inflammation and immune suppression and have been associated with a poor outcome. Plasmatic exosomes are extracellular vesicles involved in the intercellular communication system that can exert an immunosuppressive function. Aim of this study was to investigate the interplay between the immune system and circulating exosomes in metastatic breast cancer (MBC). A threshold capable to classify patients according to MLR, NLR and PLR, was computed through a receiving operator curve analysis after propensity score matching with a series of female blood donors. Exosomes were isolated from plasma by ExoQuick solution and characterized by flow-cytometry. NLR, MLR, PLR and exosomal subpopulations potentially involved in the pre-metastatic niche were significantly different in MBC patients with respect to controls. MLR was significantly associated with number of sites at the onset of metastatic disease, while high levels of MLR and NLR were found to be associated with poor prognosis. Furthermore, exosomal subpopulations varied according to NLR, MLR, PLR and both were associated with different breast cancer subtypes and sites of distant involvement. This study highlights the nuanced role of immunity in MBC spread, progression and outcome. Moreover, they suggest potential interaction mechanisms between immunity, MBC and the metastatic niche.
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spelling pubmed-71816632020-04-27 Biologically driven cut-off definition of lymphocyte ratios in metastatic breast cancer and association with exosomal subpopulations and prognosis Gerratana, Lorenzo Basile, Debora Toffoletto, Barbara Bulfoni, Michela Zago, Silvia Magini, Alessandro Lera, Marta Pelizzari, Giacomo Parisse, Pietro Casalis, Loredana Vitale, Maria Grazia Fanotto, Valentina Bonotto, Marta Caponnetto, Federica Bartoletti, Michele Lisanti, Camilla Minisini, Alessandro Marco Emiliani, Carla Di Loreto, Carla Fasola, Gianpiero Curcio, Francesco Beltrami, Antonio Paolo Cesselli, Daniela Puglisi, Fabio Sci Rep Article High neutrophil to lymphocyte ratio (NLR) and monocyte to lymphocyte ratio (MLR) are respectively associated with systemic inflammation and immune suppression and have been associated with a poor outcome. Plasmatic exosomes are extracellular vesicles involved in the intercellular communication system that can exert an immunosuppressive function. Aim of this study was to investigate the interplay between the immune system and circulating exosomes in metastatic breast cancer (MBC). A threshold capable to classify patients according to MLR, NLR and PLR, was computed through a receiving operator curve analysis after propensity score matching with a series of female blood donors. Exosomes were isolated from plasma by ExoQuick solution and characterized by flow-cytometry. NLR, MLR, PLR and exosomal subpopulations potentially involved in the pre-metastatic niche were significantly different in MBC patients with respect to controls. MLR was significantly associated with number of sites at the onset of metastatic disease, while high levels of MLR and NLR were found to be associated with poor prognosis. Furthermore, exosomal subpopulations varied according to NLR, MLR, PLR and both were associated with different breast cancer subtypes and sites of distant involvement. This study highlights the nuanced role of immunity in MBC spread, progression and outcome. Moreover, they suggest potential interaction mechanisms between immunity, MBC and the metastatic niche. Nature Publishing Group UK 2020-04-24 /pmc/articles/PMC7181663/ /pubmed/32332763 http://dx.doi.org/10.1038/s41598-020-63291-2 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Gerratana, Lorenzo
Basile, Debora
Toffoletto, Barbara
Bulfoni, Michela
Zago, Silvia
Magini, Alessandro
Lera, Marta
Pelizzari, Giacomo
Parisse, Pietro
Casalis, Loredana
Vitale, Maria Grazia
Fanotto, Valentina
Bonotto, Marta
Caponnetto, Federica
Bartoletti, Michele
Lisanti, Camilla
Minisini, Alessandro Marco
Emiliani, Carla
Di Loreto, Carla
Fasola, Gianpiero
Curcio, Francesco
Beltrami, Antonio Paolo
Cesselli, Daniela
Puglisi, Fabio
Biologically driven cut-off definition of lymphocyte ratios in metastatic breast cancer and association with exosomal subpopulations and prognosis
title Biologically driven cut-off definition of lymphocyte ratios in metastatic breast cancer and association with exosomal subpopulations and prognosis
title_full Biologically driven cut-off definition of lymphocyte ratios in metastatic breast cancer and association with exosomal subpopulations and prognosis
title_fullStr Biologically driven cut-off definition of lymphocyte ratios in metastatic breast cancer and association with exosomal subpopulations and prognosis
title_full_unstemmed Biologically driven cut-off definition of lymphocyte ratios in metastatic breast cancer and association with exosomal subpopulations and prognosis
title_short Biologically driven cut-off definition of lymphocyte ratios in metastatic breast cancer and association with exosomal subpopulations and prognosis
title_sort biologically driven cut-off definition of lymphocyte ratios in metastatic breast cancer and association with exosomal subpopulations and prognosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181663/
https://www.ncbi.nlm.nih.gov/pubmed/32332763
http://dx.doi.org/10.1038/s41598-020-63291-2
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