A simple method based on confocal microscopy and thick sections recognizes seven subphases in growth plate chondrocytes

This manuscript reports a novel procedure to imaging growth plate chondrocytes by using confocal microscopy. The method is based on fixed undecalcified bone samples, in-block staining with eosin, epoxy resin embedding and grinding to obtain thick sections. It is simple, inexpensive and provides thre...

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Detalles Bibliográficos
Autores principales: Fernández-Iglesias, Ángela, Fuente, Rocío, Gil-Peña, Helena, Alonso-Duran, Laura, García-Bengoa, María, Santos, Fernando, López, José M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181680/
https://www.ncbi.nlm.nih.gov/pubmed/32332842
http://dx.doi.org/10.1038/s41598-020-63978-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181680/
https://www.ncbi.nlm.nih.gov/pubmed/32332842
http://dx.doi.org/10.1038/s41598-020-63978-6

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