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A simple method based on confocal microscopy and thick sections recognizes seven subphases in growth plate chondrocytes
This manuscript reports a novel procedure to imaging growth plate chondrocytes by using confocal microscopy. The method is based on fixed undecalcified bone samples, in-block staining with eosin, epoxy resin embedding and grinding to obtain thick sections. It is simple, inexpensive and provides thre...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181680/ https://www.ncbi.nlm.nih.gov/pubmed/32332842 http://dx.doi.org/10.1038/s41598-020-63978-6 |