Serologic Detection of Middle East Respiratory Syndrome Coronavirus Functional Antibodies

We developed and validated 2 species-independent protein-based assays to detect Middle East respiratory syndrome coronavirus functional antibodies that can block virus receptor-binding or sialic acid-attachment. Antibody levels measured in both assays correlated strongly with virus-neutralizing anti...

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Autores principales: Okba, Nisreen M.A., Widjaja, Ivy, Li, Wentao, GeurtsvanKessel, Corine H., Farag, Elmoubasher A.B.A., Al-Hajri, Mohammed, Park, Wan Beom, Oh, Myoung-don, Reusken, Chantal B.E.M., Koopmans, Marion P.G., Bosch, Berend-Jan, Haagmans, Bart L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2020
Materias:
Research Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181916/
https://www.ncbi.nlm.nih.gov/pubmed/32150528
http://dx.doi.org/10.3201/eid2605.190921
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author Okba, Nisreen M.A.
Widjaja, Ivy
Li, Wentao
GeurtsvanKessel, Corine H.
Farag, Elmoubasher A.B.A.
Al-Hajri, Mohammed
Park, Wan Beom
Oh, Myoung-don
Reusken, Chantal B.E.M.
Koopmans, Marion P.G.
Bosch, Berend-Jan
Haagmans, Bart L.
author_facet Okba, Nisreen M.A.
Widjaja, Ivy
Li, Wentao
GeurtsvanKessel, Corine H.
Farag, Elmoubasher A.B.A.
Al-Hajri, Mohammed
Park, Wan Beom
Oh, Myoung-don
Reusken, Chantal B.E.M.
Koopmans, Marion P.G.
Bosch, Berend-Jan
Haagmans, Bart L.
author_sort Okba, Nisreen M.A.
collection PubMed
description We developed and validated 2 species-independent protein-based assays to detect Middle East respiratory syndrome coronavirus functional antibodies that can block virus receptor-binding or sialic acid-attachment. Antibody levels measured in both assays correlated strongly with virus-neutralizing antibody titers, proving their use for serologic confirmatory diagnosis of Middle East respiratory syndrome.
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spelling pubmed-71819162020-05-06 Serologic Detection of Middle East Respiratory Syndrome Coronavirus Functional Antibodies Okba, Nisreen M.A. Widjaja, Ivy Li, Wentao GeurtsvanKessel, Corine H. Farag, Elmoubasher A.B.A. Al-Hajri, Mohammed Park, Wan Beom Oh, Myoung-don Reusken, Chantal B.E.M. Koopmans, Marion P.G. Bosch, Berend-Jan Haagmans, Bart L. Emerg Infect Dis Research Letter We developed and validated 2 species-independent protein-based assays to detect Middle East respiratory syndrome coronavirus functional antibodies that can block virus receptor-binding or sialic acid-attachment. Antibody levels measured in both assays correlated strongly with virus-neutralizing antibody titers, proving their use for serologic confirmatory diagnosis of Middle East respiratory syndrome. Centers for Disease Control and Prevention 2020-05 /pmc/articles/PMC7181916/ /pubmed/32150528 http://dx.doi.org/10.3201/eid2605.190921 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research Letter
Okba, Nisreen M.A.
Widjaja, Ivy
Li, Wentao
GeurtsvanKessel, Corine H.
Farag, Elmoubasher A.B.A.
Al-Hajri, Mohammed
Park, Wan Beom
Oh, Myoung-don
Reusken, Chantal B.E.M.
Koopmans, Marion P.G.
Bosch, Berend-Jan
Haagmans, Bart L.
Serologic Detection of Middle East Respiratory Syndrome Coronavirus Functional Antibodies
title Serologic Detection of Middle East Respiratory Syndrome Coronavirus Functional Antibodies
title_full Serologic Detection of Middle East Respiratory Syndrome Coronavirus Functional Antibodies
title_fullStr Serologic Detection of Middle East Respiratory Syndrome Coronavirus Functional Antibodies
title_full_unstemmed Serologic Detection of Middle East Respiratory Syndrome Coronavirus Functional Antibodies
title_short Serologic Detection of Middle East Respiratory Syndrome Coronavirus Functional Antibodies
title_sort serologic detection of middle east respiratory syndrome coronavirus functional antibodies
topic Research Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7181916/
https://www.ncbi.nlm.nih.gov/pubmed/32150528
http://dx.doi.org/10.3201/eid2605.190921
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