Cargando…

A label-free biosensor based on graphene and reduced graphene oxide dual-layer for electrochemical determination of beta-amyloid biomarkers

A label-free biosensor is developed for the determination of plasma-based Aβ(1–42) biomarker in Alzheimer’s disease (AD). The platform is based on highly conductive dual-layer of graphene and electrochemically reduced graphene oxide (rGO). The modification of dual-layer with 1-pyrenebutyric acid N-h...

Descripción completa

Detalles Bibliográficos
Autores principales: Sethi, Jagriti, Van Bulck, Michiel, Suhail, Ahmed, Safarzadeh, Mina, Perez-Castillo, Ana, Pan, Genhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182627/
https://www.ncbi.nlm.nih.gov/pubmed/32333119
http://dx.doi.org/10.1007/s00604-020-04267-x
_version_ 1783526267347271680
author Sethi, Jagriti
Van Bulck, Michiel
Suhail, Ahmed
Safarzadeh, Mina
Perez-Castillo, Ana
Pan, Genhua
author_facet Sethi, Jagriti
Van Bulck, Michiel
Suhail, Ahmed
Safarzadeh, Mina
Perez-Castillo, Ana
Pan, Genhua
author_sort Sethi, Jagriti
collection PubMed
description A label-free biosensor is developed for the determination of plasma-based Aβ(1–42) biomarker in Alzheimer’s disease (AD). The platform is based on highly conductive dual-layer of graphene and electrochemically reduced graphene oxide (rGO). The modification of dual-layer with 1-pyrenebutyric acid N-hydroxysuccinimide ester (Pyr-NHS) is achieved to facilitate immobilization of H31L21 antibody. The effect of these modifications were studied with morphological, spectral and electrochemical techniques. The response of the biosensor was evaluated using differential pulse voltammetry (DPV). The data was acquired at a working potential of ~ 180 mV and a scan rate of 50 mV s(−1). A low limit of detection (LOD) of 2.398 pM is achieved over a wide linear range from 11 pM to 55 nM. The biosensor exhibits excellent specificity over Aβ(1–40) and ApoE ε4 interfering species. Thus, it provides a viable tool for electrochemical determination of Aβ(1–42). Spiked human and mice plasmas were used for the successful validation of the sensing platform in bio-fluidic samples. The results obtained from mice plasma analysis concurred with the immunohistochemistry (IHC) and magnetic resonance imaging (MRI) data obtained from brain analysis. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00604-020-04267-x) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-7182627
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Springer Vienna
record_format MEDLINE/PubMed
spelling pubmed-71826272020-05-04 A label-free biosensor based on graphene and reduced graphene oxide dual-layer for electrochemical determination of beta-amyloid biomarkers Sethi, Jagriti Van Bulck, Michiel Suhail, Ahmed Safarzadeh, Mina Perez-Castillo, Ana Pan, Genhua Mikrochim Acta Original Paper A label-free biosensor is developed for the determination of plasma-based Aβ(1–42) biomarker in Alzheimer’s disease (AD). The platform is based on highly conductive dual-layer of graphene and electrochemically reduced graphene oxide (rGO). The modification of dual-layer with 1-pyrenebutyric acid N-hydroxysuccinimide ester (Pyr-NHS) is achieved to facilitate immobilization of H31L21 antibody. The effect of these modifications were studied with morphological, spectral and electrochemical techniques. The response of the biosensor was evaluated using differential pulse voltammetry (DPV). The data was acquired at a working potential of ~ 180 mV and a scan rate of 50 mV s(−1). A low limit of detection (LOD) of 2.398 pM is achieved over a wide linear range from 11 pM to 55 nM. The biosensor exhibits excellent specificity over Aβ(1–40) and ApoE ε4 interfering species. Thus, it provides a viable tool for electrochemical determination of Aβ(1–42). Spiked human and mice plasmas were used for the successful validation of the sensing platform in bio-fluidic samples. The results obtained from mice plasma analysis concurred with the immunohistochemistry (IHC) and magnetic resonance imaging (MRI) data obtained from brain analysis. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00604-020-04267-x) contains supplementary material, which is available to authorized users. Springer Vienna 2020-04-25 2020 /pmc/articles/PMC7182627/ /pubmed/32333119 http://dx.doi.org/10.1007/s00604-020-04267-x Text en © The Author(s) 2020, corrected publication 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Paper
Sethi, Jagriti
Van Bulck, Michiel
Suhail, Ahmed
Safarzadeh, Mina
Perez-Castillo, Ana
Pan, Genhua
A label-free biosensor based on graphene and reduced graphene oxide dual-layer for electrochemical determination of beta-amyloid biomarkers
title A label-free biosensor based on graphene and reduced graphene oxide dual-layer for electrochemical determination of beta-amyloid biomarkers
title_full A label-free biosensor based on graphene and reduced graphene oxide dual-layer for electrochemical determination of beta-amyloid biomarkers
title_fullStr A label-free biosensor based on graphene and reduced graphene oxide dual-layer for electrochemical determination of beta-amyloid biomarkers
title_full_unstemmed A label-free biosensor based on graphene and reduced graphene oxide dual-layer for electrochemical determination of beta-amyloid biomarkers
title_short A label-free biosensor based on graphene and reduced graphene oxide dual-layer for electrochemical determination of beta-amyloid biomarkers
title_sort label-free biosensor based on graphene and reduced graphene oxide dual-layer for electrochemical determination of beta-amyloid biomarkers
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182627/
https://www.ncbi.nlm.nih.gov/pubmed/32333119
http://dx.doi.org/10.1007/s00604-020-04267-x
work_keys_str_mv AT sethijagriti alabelfreebiosensorbasedongrapheneandreducedgrapheneoxideduallayerforelectrochemicaldeterminationofbetaamyloidbiomarkers
AT vanbulckmichiel alabelfreebiosensorbasedongrapheneandreducedgrapheneoxideduallayerforelectrochemicaldeterminationofbetaamyloidbiomarkers
AT suhailahmed alabelfreebiosensorbasedongrapheneandreducedgrapheneoxideduallayerforelectrochemicaldeterminationofbetaamyloidbiomarkers
AT safarzadehmina alabelfreebiosensorbasedongrapheneandreducedgrapheneoxideduallayerforelectrochemicaldeterminationofbetaamyloidbiomarkers
AT perezcastilloana alabelfreebiosensorbasedongrapheneandreducedgrapheneoxideduallayerforelectrochemicaldeterminationofbetaamyloidbiomarkers
AT pangenhua alabelfreebiosensorbasedongrapheneandreducedgrapheneoxideduallayerforelectrochemicaldeterminationofbetaamyloidbiomarkers
AT sethijagriti labelfreebiosensorbasedongrapheneandreducedgrapheneoxideduallayerforelectrochemicaldeterminationofbetaamyloidbiomarkers
AT vanbulckmichiel labelfreebiosensorbasedongrapheneandreducedgrapheneoxideduallayerforelectrochemicaldeterminationofbetaamyloidbiomarkers
AT suhailahmed labelfreebiosensorbasedongrapheneandreducedgrapheneoxideduallayerforelectrochemicaldeterminationofbetaamyloidbiomarkers
AT safarzadehmina labelfreebiosensorbasedongrapheneandreducedgrapheneoxideduallayerforelectrochemicaldeterminationofbetaamyloidbiomarkers
AT perezcastilloana labelfreebiosensorbasedongrapheneandreducedgrapheneoxideduallayerforelectrochemicaldeterminationofbetaamyloidbiomarkers
AT pangenhua labelfreebiosensorbasedongrapheneandreducedgrapheneoxideduallayerforelectrochemicaldeterminationofbetaamyloidbiomarkers