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NAD(+) attenuates experimental autoimmune encephalomyelitis through induction of CD11b(+) gr-1(+) myeloid-derived suppressor cells
Objective: To investigate the effects of nicotinamide adenine dinucleotide (NAD(+)) on the pathogenesis of the animal model for multiple sclerosis (MS)-experimental autoimmune encephalomyelitis (EAE). Methods: EAE model was induced by myelin oligodendrocyte protein (MOG 35-55). Clinical scores of EA...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182659/ https://www.ncbi.nlm.nih.gov/pubmed/32301489 http://dx.doi.org/10.1042/BSR20200353 |
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author | Wang, Jin-Li Li, Bin Tan, Guo-Jun Gai, Xiao-Li Xing, Jun-Na Wang, Jue-Qiong Quan, Mo-Yuan Zhang, Ning Guo, Li |
author_facet | Wang, Jin-Li Li, Bin Tan, Guo-Jun Gai, Xiao-Li Xing, Jun-Na Wang, Jue-Qiong Quan, Mo-Yuan Zhang, Ning Guo, Li |
author_sort | Wang, Jin-Li |
collection | PubMed |
description | Objective: To investigate the effects of nicotinamide adenine dinucleotide (NAD(+)) on the pathogenesis of the animal model for multiple sclerosis (MS)-experimental autoimmune encephalomyelitis (EAE). Methods: EAE model was induced by myelin oligodendrocyte protein (MOG 35-55). Clinical scores of EAE were measured in mice with or without NAD(+) treatment. Hematoxylin and Eosin (HE) and Luxol Fast Blue (LFB) staining were performed to assess inflammation and demyelination, respectively. Expressions of target proteins were measured by Western blot. The numbers of myeloid-derived suppressor cells (MDSCs) were measured by immunofluorescent staining and flow cytometry. Enzyme-linked immunosorbent assay (ELISA) was used to measure the expressions of inflammatory cytokine in serum. Results: NAD(+) treatment could decrease inflammatory cells and demyelination foci, attenuate the clinical scores of EAE and slightly delay disease onset. Western blot showed that NAD(+) treatment up-regulated the expression of phosphorylated-STAT6 (p-STAT6) and SIRT1. Besides, NAD(+) treatment up-regulated the expression of p-IκB and down-regulated the expression of p-NF-κB. In addition, NAD(+) treatment could increase the numbers of CD11b(+) gr-1(+) MDSCs and the expression of Arginase-1. Moreover, NAD(+) treatment up-regulated the expressions of IL-13 and down-regulated the expression of IFN-γ and IL-17. Conclusions: The present study demonstrated that NAD(+) treatment may induce the CD11b(+) gr-1(+) MDSCs to attenuate EAE via activating the phosphorylation of STAT6 expression. Therefore, NAD(+) should be considered as a potential novel therapeutic strategy for MS. |
format | Online Article Text |
id | pubmed-7182659 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71826592020-05-05 NAD(+) attenuates experimental autoimmune encephalomyelitis through induction of CD11b(+) gr-1(+) myeloid-derived suppressor cells Wang, Jin-Li Li, Bin Tan, Guo-Jun Gai, Xiao-Li Xing, Jun-Na Wang, Jue-Qiong Quan, Mo-Yuan Zhang, Ning Guo, Li Biosci Rep Immunology & Inflammation Objective: To investigate the effects of nicotinamide adenine dinucleotide (NAD(+)) on the pathogenesis of the animal model for multiple sclerosis (MS)-experimental autoimmune encephalomyelitis (EAE). Methods: EAE model was induced by myelin oligodendrocyte protein (MOG 35-55). Clinical scores of EAE were measured in mice with or without NAD(+) treatment. Hematoxylin and Eosin (HE) and Luxol Fast Blue (LFB) staining were performed to assess inflammation and demyelination, respectively. Expressions of target proteins were measured by Western blot. The numbers of myeloid-derived suppressor cells (MDSCs) were measured by immunofluorescent staining and flow cytometry. Enzyme-linked immunosorbent assay (ELISA) was used to measure the expressions of inflammatory cytokine in serum. Results: NAD(+) treatment could decrease inflammatory cells and demyelination foci, attenuate the clinical scores of EAE and slightly delay disease onset. Western blot showed that NAD(+) treatment up-regulated the expression of phosphorylated-STAT6 (p-STAT6) and SIRT1. Besides, NAD(+) treatment up-regulated the expression of p-IκB and down-regulated the expression of p-NF-κB. In addition, NAD(+) treatment could increase the numbers of CD11b(+) gr-1(+) MDSCs and the expression of Arginase-1. Moreover, NAD(+) treatment up-regulated the expressions of IL-13 and down-regulated the expression of IFN-γ and IL-17. Conclusions: The present study demonstrated that NAD(+) treatment may induce the CD11b(+) gr-1(+) MDSCs to attenuate EAE via activating the phosphorylation of STAT6 expression. Therefore, NAD(+) should be considered as a potential novel therapeutic strategy for MS. Portland Press Ltd. 2020-04-24 /pmc/articles/PMC7182659/ /pubmed/32301489 http://dx.doi.org/10.1042/BSR20200353 Text en © 2020 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY). |
spellingShingle | Immunology & Inflammation Wang, Jin-Li Li, Bin Tan, Guo-Jun Gai, Xiao-Li Xing, Jun-Na Wang, Jue-Qiong Quan, Mo-Yuan Zhang, Ning Guo, Li NAD(+) attenuates experimental autoimmune encephalomyelitis through induction of CD11b(+) gr-1(+) myeloid-derived suppressor cells |
title | NAD(+) attenuates experimental autoimmune encephalomyelitis through induction of CD11b(+) gr-1(+) myeloid-derived suppressor cells |
title_full | NAD(+) attenuates experimental autoimmune encephalomyelitis through induction of CD11b(+) gr-1(+) myeloid-derived suppressor cells |
title_fullStr | NAD(+) attenuates experimental autoimmune encephalomyelitis through induction of CD11b(+) gr-1(+) myeloid-derived suppressor cells |
title_full_unstemmed | NAD(+) attenuates experimental autoimmune encephalomyelitis through induction of CD11b(+) gr-1(+) myeloid-derived suppressor cells |
title_short | NAD(+) attenuates experimental autoimmune encephalomyelitis through induction of CD11b(+) gr-1(+) myeloid-derived suppressor cells |
title_sort | nad(+) attenuates experimental autoimmune encephalomyelitis through induction of cd11b(+) gr-1(+) myeloid-derived suppressor cells |
topic | Immunology & Inflammation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182659/ https://www.ncbi.nlm.nih.gov/pubmed/32301489 http://dx.doi.org/10.1042/BSR20200353 |
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