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Dataset of foraminiferal sedimentary DNA (sedDNA) sequences from Svalbard

Environmental DNA (eDNA) is usually defined as genetic material obtained directly from environmental samples, such as soil, water, or ice. Coupled to DNA metabarcoding, eDNA is a powerful tool in biodiversity assessments. Results from eDNA approach provided valuable insights to the studies of past a...

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Autores principales: Pawłowska, Joanna, Pawlowski, Jan, Zajączkowski, Marek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182674/
https://www.ncbi.nlm.nih.gov/pubmed/32346576
http://dx.doi.org/10.1016/j.dib.2020.105553
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author Pawłowska, Joanna
Pawlowski, Jan
Zajączkowski, Marek
author_facet Pawłowska, Joanna
Pawlowski, Jan
Zajączkowski, Marek
author_sort Pawłowska, Joanna
collection PubMed
description Environmental DNA (eDNA) is usually defined as genetic material obtained directly from environmental samples, such as soil, water, or ice. Coupled to DNA metabarcoding, eDNA is a powerful tool in biodiversity assessments. Results from eDNA approach provided valuable insights to the studies of past and contemporary biodiversity in terrestrial and aquatic environments. However, the state and fate of eDNA are still investigated and the knowledge about the form of eDNA (i.e., extracellular vs. intracellular) or the DNA degradation under different environmental conditions is limited. Here, we tackle this issue by analyzing foraminiferal sedimentary DNA (sedDNA) from different size fractions of marine sediments: >500 µm, 500–100 µm, 100–63 µm, and < 63 µm. Surface sediment samples were collected at 15 sampling stations located in the Svalbard archipelago. Sequences of the foraminifera-specific 37f region were generated using Illumina technology. The presented data may be used as a reference for a wide range of eDNA-based studies, including biomonitoring and biodiversity assessments across time and space.
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spelling pubmed-71826742020-04-28 Dataset of foraminiferal sedimentary DNA (sedDNA) sequences from Svalbard Pawłowska, Joanna Pawlowski, Jan Zajączkowski, Marek Data Brief Biochemistry, Genetics and Molecular Biology Environmental DNA (eDNA) is usually defined as genetic material obtained directly from environmental samples, such as soil, water, or ice. Coupled to DNA metabarcoding, eDNA is a powerful tool in biodiversity assessments. Results from eDNA approach provided valuable insights to the studies of past and contemporary biodiversity in terrestrial and aquatic environments. However, the state and fate of eDNA are still investigated and the knowledge about the form of eDNA (i.e., extracellular vs. intracellular) or the DNA degradation under different environmental conditions is limited. Here, we tackle this issue by analyzing foraminiferal sedimentary DNA (sedDNA) from different size fractions of marine sediments: >500 µm, 500–100 µm, 100–63 µm, and < 63 µm. Surface sediment samples were collected at 15 sampling stations located in the Svalbard archipelago. Sequences of the foraminifera-specific 37f region were generated using Illumina technology. The presented data may be used as a reference for a wide range of eDNA-based studies, including biomonitoring and biodiversity assessments across time and space. Elsevier 2020-04-18 /pmc/articles/PMC7182674/ /pubmed/32346576 http://dx.doi.org/10.1016/j.dib.2020.105553 Text en © 2020 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Biochemistry, Genetics and Molecular Biology
Pawłowska, Joanna
Pawlowski, Jan
Zajączkowski, Marek
Dataset of foraminiferal sedimentary DNA (sedDNA) sequences from Svalbard
title Dataset of foraminiferal sedimentary DNA (sedDNA) sequences from Svalbard
title_full Dataset of foraminiferal sedimentary DNA (sedDNA) sequences from Svalbard
title_fullStr Dataset of foraminiferal sedimentary DNA (sedDNA) sequences from Svalbard
title_full_unstemmed Dataset of foraminiferal sedimentary DNA (sedDNA) sequences from Svalbard
title_short Dataset of foraminiferal sedimentary DNA (sedDNA) sequences from Svalbard
title_sort dataset of foraminiferal sedimentary dna (seddna) sequences from svalbard
topic Biochemistry, Genetics and Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182674/
https://www.ncbi.nlm.nih.gov/pubmed/32346576
http://dx.doi.org/10.1016/j.dib.2020.105553
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