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An Active and Soft Hydrogel Actuator to Stimulate Live Cell Clusters by Self-folding

The hydrogels are widely used in various applications, and their successful uses depend on controlling the mechanical properties. In this study, we present an advanced strategy to develop hydrogel actuator designed to stimulate live cell clusters by self-folding. The hydrogel actuator consisting of...

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Autores principales: Lim, Jun Woo, Kim, Hee-jin, Kim, Yechan, Shin, Sung Gyu, Cho, Sungwoo, Jung, Woong Gyu, Jeong, Jae Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182895/
https://www.ncbi.nlm.nih.gov/pubmed/32150989
http://dx.doi.org/10.3390/polym12030583
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author Lim, Jun Woo
Kim, Hee-jin
Kim, Yechan
Shin, Sung Gyu
Cho, Sungwoo
Jung, Woong Gyu
Jeong, Jae Hyun
author_facet Lim, Jun Woo
Kim, Hee-jin
Kim, Yechan
Shin, Sung Gyu
Cho, Sungwoo
Jung, Woong Gyu
Jeong, Jae Hyun
author_sort Lim, Jun Woo
collection PubMed
description The hydrogels are widely used in various applications, and their successful uses depend on controlling the mechanical properties. In this study, we present an advanced strategy to develop hydrogel actuator designed to stimulate live cell clusters by self-folding. The hydrogel actuator consisting of two layers with different expansion ratios were fabricated to have various curvatures in self-folding. The expansion ratio of the hydrogel tuned with the molecular weight and concentration of gel-forming polymers, and temperature-sensitive molecules in a controlled manner. As a result, the hydrogel actuator could stimulate live cell clusters by compression and tension repeatedly, in response to temperature. The cell clusters were compressed in the 0.7-fold decreases of the radius of curvature with 1.0 mm in room temperature, as compared to that of 1.4 mm in 37 °C. Interestingly, the vascular endothelial growth factor (VEGF) and insulin-like growth factor-binding protein-2 (IGFBP-2) in MCF-7 tumor cells exposed by mechanical stimulation was expressed more than in those without stimulation. Overall, this new strategy to prepare the active and soft hydrogel actuator would be actively used in tissue engineering, drug delivery, and micro-scale actuators.
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spelling pubmed-71828952020-05-01 An Active and Soft Hydrogel Actuator to Stimulate Live Cell Clusters by Self-folding Lim, Jun Woo Kim, Hee-jin Kim, Yechan Shin, Sung Gyu Cho, Sungwoo Jung, Woong Gyu Jeong, Jae Hyun Polymers (Basel) Article The hydrogels are widely used in various applications, and their successful uses depend on controlling the mechanical properties. In this study, we present an advanced strategy to develop hydrogel actuator designed to stimulate live cell clusters by self-folding. The hydrogel actuator consisting of two layers with different expansion ratios were fabricated to have various curvatures in self-folding. The expansion ratio of the hydrogel tuned with the molecular weight and concentration of gel-forming polymers, and temperature-sensitive molecules in a controlled manner. As a result, the hydrogel actuator could stimulate live cell clusters by compression and tension repeatedly, in response to temperature. The cell clusters were compressed in the 0.7-fold decreases of the radius of curvature with 1.0 mm in room temperature, as compared to that of 1.4 mm in 37 °C. Interestingly, the vascular endothelial growth factor (VEGF) and insulin-like growth factor-binding protein-2 (IGFBP-2) in MCF-7 tumor cells exposed by mechanical stimulation was expressed more than in those without stimulation. Overall, this new strategy to prepare the active and soft hydrogel actuator would be actively used in tissue engineering, drug delivery, and micro-scale actuators. MDPI 2020-03-05 /pmc/articles/PMC7182895/ /pubmed/32150989 http://dx.doi.org/10.3390/polym12030583 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lim, Jun Woo
Kim, Hee-jin
Kim, Yechan
Shin, Sung Gyu
Cho, Sungwoo
Jung, Woong Gyu
Jeong, Jae Hyun
An Active and Soft Hydrogel Actuator to Stimulate Live Cell Clusters by Self-folding
title An Active and Soft Hydrogel Actuator to Stimulate Live Cell Clusters by Self-folding
title_full An Active and Soft Hydrogel Actuator to Stimulate Live Cell Clusters by Self-folding
title_fullStr An Active and Soft Hydrogel Actuator to Stimulate Live Cell Clusters by Self-folding
title_full_unstemmed An Active and Soft Hydrogel Actuator to Stimulate Live Cell Clusters by Self-folding
title_short An Active and Soft Hydrogel Actuator to Stimulate Live Cell Clusters by Self-folding
title_sort active and soft hydrogel actuator to stimulate live cell clusters by self-folding
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182895/
https://www.ncbi.nlm.nih.gov/pubmed/32150989
http://dx.doi.org/10.3390/polym12030583
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