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An Active and Soft Hydrogel Actuator to Stimulate Live Cell Clusters by Self-folding
The hydrogels are widely used in various applications, and their successful uses depend on controlling the mechanical properties. In this study, we present an advanced strategy to develop hydrogel actuator designed to stimulate live cell clusters by self-folding. The hydrogel actuator consisting of...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182895/ https://www.ncbi.nlm.nih.gov/pubmed/32150989 http://dx.doi.org/10.3390/polym12030583 |
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author | Lim, Jun Woo Kim, Hee-jin Kim, Yechan Shin, Sung Gyu Cho, Sungwoo Jung, Woong Gyu Jeong, Jae Hyun |
author_facet | Lim, Jun Woo Kim, Hee-jin Kim, Yechan Shin, Sung Gyu Cho, Sungwoo Jung, Woong Gyu Jeong, Jae Hyun |
author_sort | Lim, Jun Woo |
collection | PubMed |
description | The hydrogels are widely used in various applications, and their successful uses depend on controlling the mechanical properties. In this study, we present an advanced strategy to develop hydrogel actuator designed to stimulate live cell clusters by self-folding. The hydrogel actuator consisting of two layers with different expansion ratios were fabricated to have various curvatures in self-folding. The expansion ratio of the hydrogel tuned with the molecular weight and concentration of gel-forming polymers, and temperature-sensitive molecules in a controlled manner. As a result, the hydrogel actuator could stimulate live cell clusters by compression and tension repeatedly, in response to temperature. The cell clusters were compressed in the 0.7-fold decreases of the radius of curvature with 1.0 mm in room temperature, as compared to that of 1.4 mm in 37 °C. Interestingly, the vascular endothelial growth factor (VEGF) and insulin-like growth factor-binding protein-2 (IGFBP-2) in MCF-7 tumor cells exposed by mechanical stimulation was expressed more than in those without stimulation. Overall, this new strategy to prepare the active and soft hydrogel actuator would be actively used in tissue engineering, drug delivery, and micro-scale actuators. |
format | Online Article Text |
id | pubmed-7182895 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71828952020-05-01 An Active and Soft Hydrogel Actuator to Stimulate Live Cell Clusters by Self-folding Lim, Jun Woo Kim, Hee-jin Kim, Yechan Shin, Sung Gyu Cho, Sungwoo Jung, Woong Gyu Jeong, Jae Hyun Polymers (Basel) Article The hydrogels are widely used in various applications, and their successful uses depend on controlling the mechanical properties. In this study, we present an advanced strategy to develop hydrogel actuator designed to stimulate live cell clusters by self-folding. The hydrogel actuator consisting of two layers with different expansion ratios were fabricated to have various curvatures in self-folding. The expansion ratio of the hydrogel tuned with the molecular weight and concentration of gel-forming polymers, and temperature-sensitive molecules in a controlled manner. As a result, the hydrogel actuator could stimulate live cell clusters by compression and tension repeatedly, in response to temperature. The cell clusters were compressed in the 0.7-fold decreases of the radius of curvature with 1.0 mm in room temperature, as compared to that of 1.4 mm in 37 °C. Interestingly, the vascular endothelial growth factor (VEGF) and insulin-like growth factor-binding protein-2 (IGFBP-2) in MCF-7 tumor cells exposed by mechanical stimulation was expressed more than in those without stimulation. Overall, this new strategy to prepare the active and soft hydrogel actuator would be actively used in tissue engineering, drug delivery, and micro-scale actuators. MDPI 2020-03-05 /pmc/articles/PMC7182895/ /pubmed/32150989 http://dx.doi.org/10.3390/polym12030583 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lim, Jun Woo Kim, Hee-jin Kim, Yechan Shin, Sung Gyu Cho, Sungwoo Jung, Woong Gyu Jeong, Jae Hyun An Active and Soft Hydrogel Actuator to Stimulate Live Cell Clusters by Self-folding |
title | An Active and Soft Hydrogel Actuator to Stimulate Live Cell Clusters by Self-folding |
title_full | An Active and Soft Hydrogel Actuator to Stimulate Live Cell Clusters by Self-folding |
title_fullStr | An Active and Soft Hydrogel Actuator to Stimulate Live Cell Clusters by Self-folding |
title_full_unstemmed | An Active and Soft Hydrogel Actuator to Stimulate Live Cell Clusters by Self-folding |
title_short | An Active and Soft Hydrogel Actuator to Stimulate Live Cell Clusters by Self-folding |
title_sort | active and soft hydrogel actuator to stimulate live cell clusters by self-folding |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182895/ https://www.ncbi.nlm.nih.gov/pubmed/32150989 http://dx.doi.org/10.3390/polym12030583 |
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