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A Novel Multilayer Composite Membrane for Wound Healing in Mice Skin Defect Model
To develop a wound dressing material that conforms to the healing process, we prepared a multilayer composite (MC) membrane consisting of an antibacterial layer (ABL), a reinforcement layer (RFL), and a healing promotion layer (HPL). Biocompatible zein/ethyl cellulose (zein/EC) electrospun nanofibro...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182948/ https://www.ncbi.nlm.nih.gov/pubmed/32143489 http://dx.doi.org/10.3390/polym12030573 |
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author | Qiu, Yuyu Wang, Qingqing Chen, Yajun Xia, Shufang Huang, Wei Wei, Qufu |
author_facet | Qiu, Yuyu Wang, Qingqing Chen, Yajun Xia, Shufang Huang, Wei Wei, Qufu |
author_sort | Qiu, Yuyu |
collection | PubMed |
description | To develop a wound dressing material that conforms to the healing process, we prepared a multilayer composite (MC) membrane consisting of an antibacterial layer (ABL), a reinforcement layer (RFL), and a healing promotion layer (HPL). Biocompatible zein/ethyl cellulose (zein/EC) electrospun nanofibrous membranes with in situ loaded antibacterial photosensitizer protoporphyrin (PPIX) and healing promotion material vaccarin (Vac) were, respectively, chosen as the ABL on the surface and the HPL on the bottom, between which nonwoven incorporated bacterial cellulose (BC/PETN) as the HPL was intercalated to enhance the mechanical property. Photodynamic antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa was confirmed by the enlarged inhibition zones; meanwhile, satisfactory biocompatibility of the HPL was verified by scanning electronic microscopy (SEM) of L929 cells cultured on its surface. The potential effects on wound healing in a mice skin defect model of the MC membranes were also evaluated. The animal experiments demonstrated that the wound healing rate in the MC group was significantly increased compared with that in the control group (p < 0.05). Histopathological observation revealed an alleviated inflammatory response, accompanied with vascular proliferation in the MC group. The MC membranes significantly promoted wound healing by creating an antibacterial environment and promoting angiogenesis. Taken together, this MC membrane may act as a promising wound dressing for skin wound healing. |
format | Online Article Text |
id | pubmed-7182948 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-71829482020-05-01 A Novel Multilayer Composite Membrane for Wound Healing in Mice Skin Defect Model Qiu, Yuyu Wang, Qingqing Chen, Yajun Xia, Shufang Huang, Wei Wei, Qufu Polymers (Basel) Article To develop a wound dressing material that conforms to the healing process, we prepared a multilayer composite (MC) membrane consisting of an antibacterial layer (ABL), a reinforcement layer (RFL), and a healing promotion layer (HPL). Biocompatible zein/ethyl cellulose (zein/EC) electrospun nanofibrous membranes with in situ loaded antibacterial photosensitizer protoporphyrin (PPIX) and healing promotion material vaccarin (Vac) were, respectively, chosen as the ABL on the surface and the HPL on the bottom, between which nonwoven incorporated bacterial cellulose (BC/PETN) as the HPL was intercalated to enhance the mechanical property. Photodynamic antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa was confirmed by the enlarged inhibition zones; meanwhile, satisfactory biocompatibility of the HPL was verified by scanning electronic microscopy (SEM) of L929 cells cultured on its surface. The potential effects on wound healing in a mice skin defect model of the MC membranes were also evaluated. The animal experiments demonstrated that the wound healing rate in the MC group was significantly increased compared with that in the control group (p < 0.05). Histopathological observation revealed an alleviated inflammatory response, accompanied with vascular proliferation in the MC group. The MC membranes significantly promoted wound healing by creating an antibacterial environment and promoting angiogenesis. Taken together, this MC membrane may act as a promising wound dressing for skin wound healing. MDPI 2020-03-04 /pmc/articles/PMC7182948/ /pubmed/32143489 http://dx.doi.org/10.3390/polym12030573 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Qiu, Yuyu Wang, Qingqing Chen, Yajun Xia, Shufang Huang, Wei Wei, Qufu A Novel Multilayer Composite Membrane for Wound Healing in Mice Skin Defect Model |
title | A Novel Multilayer Composite Membrane for Wound Healing in Mice Skin Defect Model |
title_full | A Novel Multilayer Composite Membrane for Wound Healing in Mice Skin Defect Model |
title_fullStr | A Novel Multilayer Composite Membrane for Wound Healing in Mice Skin Defect Model |
title_full_unstemmed | A Novel Multilayer Composite Membrane for Wound Healing in Mice Skin Defect Model |
title_short | A Novel Multilayer Composite Membrane for Wound Healing in Mice Skin Defect Model |
title_sort | novel multilayer composite membrane for wound healing in mice skin defect model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182948/ https://www.ncbi.nlm.nih.gov/pubmed/32143489 http://dx.doi.org/10.3390/polym12030573 |
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