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Impact of ethanolic extract of Equisetum arvense (EA1) on pancreatic carcinoma AsPC-1 cells

The current research was focused on evaluation of the cytotoxic and suppressive action of ethanolic extract of Equisetum arvense (EA1) against human pancreatic carcinoma cell line ASPC-1 after treatment with 25 µg/mL, 50 µg/mL, 100 µg/mL and 200 µg/mL EA1, using MTT assay and Antioxidant activity. D...

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Detalles Bibliográficos
Autores principales: Bhat, Ajaz Ali, Ahamad, Bilal, Rehman, Muneeb U., Ahmad, Parvaiz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7183007/
https://www.ncbi.nlm.nih.gov/pubmed/32346333
http://dx.doi.org/10.1016/j.sjbs.2020.01.029
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author Bhat, Ajaz Ali
Ahamad, Bilal
Rehman, Muneeb U.
Ahmad, Parvaiz
author_facet Bhat, Ajaz Ali
Ahamad, Bilal
Rehman, Muneeb U.
Ahmad, Parvaiz
author_sort Bhat, Ajaz Ali
collection PubMed
description The current research was focused on evaluation of the cytotoxic and suppressive action of ethanolic extract of Equisetum arvense (EA1) against human pancreatic carcinoma cell line ASPC-1 after treatment with 25 µg/mL, 50 µg/mL, 100 µg/mL and 200 µg/mL EA1, using MTT assay and Antioxidant activity. Detailed investigations led to reveal the ability of cell patronage through the dreadful upshot of free radicals. The current approach followed MTT assays to examine the long-lasting ability and growth of cells as EA1 restrained the cell viability and growth of ASPC-1. At the end, EA1 showed its potential cytotoxicity and reduced the cellular proliferation of ASPC-1 cells through a pattern, which appeared to be concentration dependent. Our results can form the basis to explore the molecular mechanisms underlying Ethanolic Extract of Equisetum arvense induced cell death in pancreatic cancer cell lines and may serve as an alternative anticancer agent for the treatment of pancreatic carcinoma (PC) with no or least side effects to the patient.
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spelling pubmed-71830072020-04-28 Impact of ethanolic extract of Equisetum arvense (EA1) on pancreatic carcinoma AsPC-1 cells Bhat, Ajaz Ali Ahamad, Bilal Rehman, Muneeb U. Ahmad, Parvaiz Saudi J Biol Sci Article The current research was focused on evaluation of the cytotoxic and suppressive action of ethanolic extract of Equisetum arvense (EA1) against human pancreatic carcinoma cell line ASPC-1 after treatment with 25 µg/mL, 50 µg/mL, 100 µg/mL and 200 µg/mL EA1, using MTT assay and Antioxidant activity. Detailed investigations led to reveal the ability of cell patronage through the dreadful upshot of free radicals. The current approach followed MTT assays to examine the long-lasting ability and growth of cells as EA1 restrained the cell viability and growth of ASPC-1. At the end, EA1 showed its potential cytotoxicity and reduced the cellular proliferation of ASPC-1 cells through a pattern, which appeared to be concentration dependent. Our results can form the basis to explore the molecular mechanisms underlying Ethanolic Extract of Equisetum arvense induced cell death in pancreatic cancer cell lines and may serve as an alternative anticancer agent for the treatment of pancreatic carcinoma (PC) with no or least side effects to the patient. Elsevier 2020-05 2020-01-30 /pmc/articles/PMC7183007/ /pubmed/32346333 http://dx.doi.org/10.1016/j.sjbs.2020.01.029 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Bhat, Ajaz Ali
Ahamad, Bilal
Rehman, Muneeb U.
Ahmad, Parvaiz
Impact of ethanolic extract of Equisetum arvense (EA1) on pancreatic carcinoma AsPC-1 cells
title Impact of ethanolic extract of Equisetum arvense (EA1) on pancreatic carcinoma AsPC-1 cells
title_full Impact of ethanolic extract of Equisetum arvense (EA1) on pancreatic carcinoma AsPC-1 cells
title_fullStr Impact of ethanolic extract of Equisetum arvense (EA1) on pancreatic carcinoma AsPC-1 cells
title_full_unstemmed Impact of ethanolic extract of Equisetum arvense (EA1) on pancreatic carcinoma AsPC-1 cells
title_short Impact of ethanolic extract of Equisetum arvense (EA1) on pancreatic carcinoma AsPC-1 cells
title_sort impact of ethanolic extract of equisetum arvense (ea1) on pancreatic carcinoma aspc-1 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7183007/
https://www.ncbi.nlm.nih.gov/pubmed/32346333
http://dx.doi.org/10.1016/j.sjbs.2020.01.029
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