Cargando…

Switchable Lipid Provides pH-Sensitive Properties to Lipid and Hybrid Polymer/Lipid Membranes

Blending amphiphilic copolymers and lipids constitutes a novel approach to combine the advantages of polymersomes and liposomes into a new single hybrid membrane. Efforts have been made to design stimuli-responsive vesicles, in which the membrane’s dynamic is modulated by specific triggers. In this...

Descripción completa

Detalles Bibliográficos
Autores principales: Passos Gibson, Victor, Fauquignon, Martin, Ibarboure, Emmanuel, Leblond Chain, Jeanne, Le Meins, Jean-François
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7183064/
https://www.ncbi.nlm.nih.gov/pubmed/32168824
http://dx.doi.org/10.3390/polym12030637
_version_ 1783526356834844672
author Passos Gibson, Victor
Fauquignon, Martin
Ibarboure, Emmanuel
Leblond Chain, Jeanne
Le Meins, Jean-François
author_facet Passos Gibson, Victor
Fauquignon, Martin
Ibarboure, Emmanuel
Leblond Chain, Jeanne
Le Meins, Jean-François
author_sort Passos Gibson, Victor
collection PubMed
description Blending amphiphilic copolymers and lipids constitutes a novel approach to combine the advantages of polymersomes and liposomes into a new single hybrid membrane. Efforts have been made to design stimuli-responsive vesicles, in which the membrane’s dynamic is modulated by specific triggers. In this investigation, we proposed the design of pH-responsive hybrid vesicles formulated with poly(dimethylsiloxane)-block-poly(ethylene oxide) backbone (PDMS(36)-b-PEO(23)) and cationic switchable lipid (CSL). The latter undergoes a pH-triggered conformational change and induces membrane destabilization. Using confocal imaging and DLS measurements, we interrogated the structural changes in CSL-doped lipid and hybrid polymer/lipid unilamellar vesicles at the micro- and nanometric scale, respectively. Both switchable giant unilamellar lipid vesicles (GUV) and hybrid polymer/lipid unilamellar vesicles (GHUV) presented dynamic morphological changes, including protrusions and fission upon acidification. At the submicron scale, scattered intensity decreased for both switchable large unilamellar vesicles (LUV) and hybrid vesicles (LHUV) under acidic pH. Finally, monitoring the fluorescence leakage of encapsulated calcein, we attested that CSL increased the permeability of GUV and GHUV in a pH-specific fashion. Altogether, these results show that switchable lipids provide a pH-sensitive behavior to hybrid polymer/lipid vesicles that could be exploited for the triggered release of drugs, cell biomimicry studies, or as bioinspired micro/nanoreactors.
format Online
Article
Text
id pubmed-7183064
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-71830642020-05-01 Switchable Lipid Provides pH-Sensitive Properties to Lipid and Hybrid Polymer/Lipid Membranes Passos Gibson, Victor Fauquignon, Martin Ibarboure, Emmanuel Leblond Chain, Jeanne Le Meins, Jean-François Polymers (Basel) Article Blending amphiphilic copolymers and lipids constitutes a novel approach to combine the advantages of polymersomes and liposomes into a new single hybrid membrane. Efforts have been made to design stimuli-responsive vesicles, in which the membrane’s dynamic is modulated by specific triggers. In this investigation, we proposed the design of pH-responsive hybrid vesicles formulated with poly(dimethylsiloxane)-block-poly(ethylene oxide) backbone (PDMS(36)-b-PEO(23)) and cationic switchable lipid (CSL). The latter undergoes a pH-triggered conformational change and induces membrane destabilization. Using confocal imaging and DLS measurements, we interrogated the structural changes in CSL-doped lipid and hybrid polymer/lipid unilamellar vesicles at the micro- and nanometric scale, respectively. Both switchable giant unilamellar lipid vesicles (GUV) and hybrid polymer/lipid unilamellar vesicles (GHUV) presented dynamic morphological changes, including protrusions and fission upon acidification. At the submicron scale, scattered intensity decreased for both switchable large unilamellar vesicles (LUV) and hybrid vesicles (LHUV) under acidic pH. Finally, monitoring the fluorescence leakage of encapsulated calcein, we attested that CSL increased the permeability of GUV and GHUV in a pH-specific fashion. Altogether, these results show that switchable lipids provide a pH-sensitive behavior to hybrid polymer/lipid vesicles that could be exploited for the triggered release of drugs, cell biomimicry studies, or as bioinspired micro/nanoreactors. MDPI 2020-03-11 /pmc/articles/PMC7183064/ /pubmed/32168824 http://dx.doi.org/10.3390/polym12030637 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Passos Gibson, Victor
Fauquignon, Martin
Ibarboure, Emmanuel
Leblond Chain, Jeanne
Le Meins, Jean-François
Switchable Lipid Provides pH-Sensitive Properties to Lipid and Hybrid Polymer/Lipid Membranes
title Switchable Lipid Provides pH-Sensitive Properties to Lipid and Hybrid Polymer/Lipid Membranes
title_full Switchable Lipid Provides pH-Sensitive Properties to Lipid and Hybrid Polymer/Lipid Membranes
title_fullStr Switchable Lipid Provides pH-Sensitive Properties to Lipid and Hybrid Polymer/Lipid Membranes
title_full_unstemmed Switchable Lipid Provides pH-Sensitive Properties to Lipid and Hybrid Polymer/Lipid Membranes
title_short Switchable Lipid Provides pH-Sensitive Properties to Lipid and Hybrid Polymer/Lipid Membranes
title_sort switchable lipid provides ph-sensitive properties to lipid and hybrid polymer/lipid membranes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7183064/
https://www.ncbi.nlm.nih.gov/pubmed/32168824
http://dx.doi.org/10.3390/polym12030637
work_keys_str_mv AT passosgibsonvictor switchablelipidprovidesphsensitivepropertiestolipidandhybridpolymerlipidmembranes
AT fauquignonmartin switchablelipidprovidesphsensitivepropertiestolipidandhybridpolymerlipidmembranes
AT ibarboureemmanuel switchablelipidprovidesphsensitivepropertiestolipidandhybridpolymerlipidmembranes
AT leblondchainjeanne switchablelipidprovidesphsensitivepropertiestolipidandhybridpolymerlipidmembranes
AT lemeinsjeanfrancois switchablelipidprovidesphsensitivepropertiestolipidandhybridpolymerlipidmembranes