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Material Characterization of PCL:PLLA Electrospun Fibers Following Six Months Degradation In Vitro

The annulus fibrosus—one of the two tissues comprising the intervertebral disc—is susceptible to injury and disease, leading to chronic pain and rupture. A synthetic, biodegradable material could provide a suitable scaffold that alleviates this pain and supports repair through tissue regeneration. T...

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Autores principales: Shamsah, Alyah H., Cartmell, Sarah H., Richardson, Stephen M., Bosworth, Lucy A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7183275/
https://www.ncbi.nlm.nih.gov/pubmed/32245277
http://dx.doi.org/10.3390/polym12030700
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author Shamsah, Alyah H.
Cartmell, Sarah H.
Richardson, Stephen M.
Bosworth, Lucy A.
author_facet Shamsah, Alyah H.
Cartmell, Sarah H.
Richardson, Stephen M.
Bosworth, Lucy A.
author_sort Shamsah, Alyah H.
collection PubMed
description The annulus fibrosus—one of the two tissues comprising the intervertebral disc—is susceptible to injury and disease, leading to chronic pain and rupture. A synthetic, biodegradable material could provide a suitable scaffold that alleviates this pain and supports repair through tissue regeneration. The transfer of properties, particularly biomechanical, from scaffold to new tissue is essential and should occur at the same rate to prevent graft failure post-implantation. This study outlines the effect of hydrolytic degradation on the material properties of a novel blend of polycaprolactone and poly(lactic acid) electrospun nanofibers (50:50) over a six-month period following storage in phosphate buffered saline solution at 37 °C. As expected, the molecular weight distribution for this blend decreased over the 180-day period. This was in line with significant changes to fiber morphology, which appeared swollen and merged following observation using Scanning Electron Microscopy. Similarly, hydrolysis resulted in considerable remodeling of the scaffolds’ polymer chains as demonstrated by sharp increases in percentage crystallinity and tensile properties becoming stiffer, stronger and more brittle over time. These mechanical data remained within the range reported for human annulus fibrosus tissue and their long-term efficacy further supports this novel blend as a potential scaffold to support tissue regeneration.
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spelling pubmed-71832752020-05-01 Material Characterization of PCL:PLLA Electrospun Fibers Following Six Months Degradation In Vitro Shamsah, Alyah H. Cartmell, Sarah H. Richardson, Stephen M. Bosworth, Lucy A. Polymers (Basel) Article The annulus fibrosus—one of the two tissues comprising the intervertebral disc—is susceptible to injury and disease, leading to chronic pain and rupture. A synthetic, biodegradable material could provide a suitable scaffold that alleviates this pain and supports repair through tissue regeneration. The transfer of properties, particularly biomechanical, from scaffold to new tissue is essential and should occur at the same rate to prevent graft failure post-implantation. This study outlines the effect of hydrolytic degradation on the material properties of a novel blend of polycaprolactone and poly(lactic acid) electrospun nanofibers (50:50) over a six-month period following storage in phosphate buffered saline solution at 37 °C. As expected, the molecular weight distribution for this blend decreased over the 180-day period. This was in line with significant changes to fiber morphology, which appeared swollen and merged following observation using Scanning Electron Microscopy. Similarly, hydrolysis resulted in considerable remodeling of the scaffolds’ polymer chains as demonstrated by sharp increases in percentage crystallinity and tensile properties becoming stiffer, stronger and more brittle over time. These mechanical data remained within the range reported for human annulus fibrosus tissue and their long-term efficacy further supports this novel blend as a potential scaffold to support tissue regeneration. MDPI 2020-03-21 /pmc/articles/PMC7183275/ /pubmed/32245277 http://dx.doi.org/10.3390/polym12030700 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shamsah, Alyah H.
Cartmell, Sarah H.
Richardson, Stephen M.
Bosworth, Lucy A.
Material Characterization of PCL:PLLA Electrospun Fibers Following Six Months Degradation In Vitro
title Material Characterization of PCL:PLLA Electrospun Fibers Following Six Months Degradation In Vitro
title_full Material Characterization of PCL:PLLA Electrospun Fibers Following Six Months Degradation In Vitro
title_fullStr Material Characterization of PCL:PLLA Electrospun Fibers Following Six Months Degradation In Vitro
title_full_unstemmed Material Characterization of PCL:PLLA Electrospun Fibers Following Six Months Degradation In Vitro
title_short Material Characterization of PCL:PLLA Electrospun Fibers Following Six Months Degradation In Vitro
title_sort material characterization of pcl:plla electrospun fibers following six months degradation in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7183275/
https://www.ncbi.nlm.nih.gov/pubmed/32245277
http://dx.doi.org/10.3390/polym12030700
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