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Antibacterial and cytotoxic activity assessment of Channa striatus (Haruan) extract

BACKGROUND AND AIM: Channa striatus extract, a freshwater snakehead fish known as Haruan, is popular in Southeast Asia for consumption and as a traditional therapeutic remedy for wound healing. C. striatus is also used in osteoarthritic for its anti-inflammatory. The aim of this study was to determi...

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Autores principales: Mat Zawawi, Nur Zulaikha, Shaari, Rumaizi, Luqman Nordin, Muhammad, Hayati Hamdan, Ruhil, Peng, Tan Li, Zalati, C. W. Salma C. W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7183459/
https://www.ncbi.nlm.nih.gov/pubmed/32367957
http://dx.doi.org/10.14202/vetworld.2020.508-514
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author Mat Zawawi, Nur Zulaikha
Shaari, Rumaizi
Luqman Nordin, Muhammad
Hayati Hamdan, Ruhil
Peng, Tan Li
Zalati, C. W. Salma C. W.
author_facet Mat Zawawi, Nur Zulaikha
Shaari, Rumaizi
Luqman Nordin, Muhammad
Hayati Hamdan, Ruhil
Peng, Tan Li
Zalati, C. W. Salma C. W.
author_sort Mat Zawawi, Nur Zulaikha
collection PubMed
description BACKGROUND AND AIM: Channa striatus extract, a freshwater snakehead fish known as Haruan, is popular in Southeast Asia for consumption and as a traditional therapeutic remedy for wound healing. C. striatus is also used in osteoarthritic for its anti-inflammatory. The aim of this study was to determine the presence of antibacterial properties of C. striatus extract against oral bacteria and to investigate the cytotoxic activity against Vero cells. MATERIALS AND METHODS: The authors prepared C. striatus extract in chloroform-methanol solvents. Next, the authors took subgingival microbiological samples from 16 cats that had periodontal disease. The authors determined the antibacterial properties of C. striatus extract against the isolated bacteria using the disk diffusion method and a broth microdilution-based resazurin microtiter assay. Finally, the authors used the Vero cell line to evaluate the cytotoxic activity, and they assessed the cell availability using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. RESULTS: The results showed weak antibacterial activity of C. striatus extract against Pseudomonas spp. and Escherichia coli. In addition, the authors found that minimum inhibition concentration values ranged between 400 and 500 mg/mL, and minimum bactericidal concentration values ranged between 650 and 550 mg/mL. However, the cytotoxic results were promising, showing that C. striatus extract increased the cell viability and growth when it was at a higher concentration. The extract also promotes growth and cell proliferation. CONCLUSION: These findings suggest that C. striatus extract promoted cell proliferation in vitro and could be a plausible therapeutic wound healing alternative for periodontal disease in cats.
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spelling pubmed-71834592020-05-04 Antibacterial and cytotoxic activity assessment of Channa striatus (Haruan) extract Mat Zawawi, Nur Zulaikha Shaari, Rumaizi Luqman Nordin, Muhammad Hayati Hamdan, Ruhil Peng, Tan Li Zalati, C. W. Salma C. W. Vet World Research Article BACKGROUND AND AIM: Channa striatus extract, a freshwater snakehead fish known as Haruan, is popular in Southeast Asia for consumption and as a traditional therapeutic remedy for wound healing. C. striatus is also used in osteoarthritic for its anti-inflammatory. The aim of this study was to determine the presence of antibacterial properties of C. striatus extract against oral bacteria and to investigate the cytotoxic activity against Vero cells. MATERIALS AND METHODS: The authors prepared C. striatus extract in chloroform-methanol solvents. Next, the authors took subgingival microbiological samples from 16 cats that had periodontal disease. The authors determined the antibacterial properties of C. striatus extract against the isolated bacteria using the disk diffusion method and a broth microdilution-based resazurin microtiter assay. Finally, the authors used the Vero cell line to evaluate the cytotoxic activity, and they assessed the cell availability using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. RESULTS: The results showed weak antibacterial activity of C. striatus extract against Pseudomonas spp. and Escherichia coli. In addition, the authors found that minimum inhibition concentration values ranged between 400 and 500 mg/mL, and minimum bactericidal concentration values ranged between 650 and 550 mg/mL. However, the cytotoxic results were promising, showing that C. striatus extract increased the cell viability and growth when it was at a higher concentration. The extract also promotes growth and cell proliferation. CONCLUSION: These findings suggest that C. striatus extract promoted cell proliferation in vitro and could be a plausible therapeutic wound healing alternative for periodontal disease in cats. Veterinary World 2020-03 2020-03-19 /pmc/articles/PMC7183459/ /pubmed/32367957 http://dx.doi.org/10.14202/vetworld.2020.508-514 Text en Copyright: © Zawawi, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Mat Zawawi, Nur Zulaikha
Shaari, Rumaizi
Luqman Nordin, Muhammad
Hayati Hamdan, Ruhil
Peng, Tan Li
Zalati, C. W. Salma C. W.
Antibacterial and cytotoxic activity assessment of Channa striatus (Haruan) extract
title Antibacterial and cytotoxic activity assessment of Channa striatus (Haruan) extract
title_full Antibacterial and cytotoxic activity assessment of Channa striatus (Haruan) extract
title_fullStr Antibacterial and cytotoxic activity assessment of Channa striatus (Haruan) extract
title_full_unstemmed Antibacterial and cytotoxic activity assessment of Channa striatus (Haruan) extract
title_short Antibacterial and cytotoxic activity assessment of Channa striatus (Haruan) extract
title_sort antibacterial and cytotoxic activity assessment of channa striatus (haruan) extract
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7183459/
https://www.ncbi.nlm.nih.gov/pubmed/32367957
http://dx.doi.org/10.14202/vetworld.2020.508-514
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