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Engineered butyrate-producing bacteria prevents high fat diet-induced obesity in mice
BACKGROUND: Obesity is a major problem worldwide and severely affects public safety. As a metabolite of gut microbiota, endogenous butyric acid participates in energy and material metabolism. Considering the serious side effects and weight regain associated with existing weight loss interventions, n...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7183672/ https://www.ncbi.nlm.nih.gov/pubmed/32334588 http://dx.doi.org/10.1186/s12934-020-01350-z |
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author | Bai, Liang Gao, Mengxue Cheng, Xiaoming Kang, Guangbo Cao, Xiaocang Huang, He |
author_facet | Bai, Liang Gao, Mengxue Cheng, Xiaoming Kang, Guangbo Cao, Xiaocang Huang, He |
author_sort | Bai, Liang |
collection | PubMed |
description | BACKGROUND: Obesity is a major problem worldwide and severely affects public safety. As a metabolite of gut microbiota, endogenous butyric acid participates in energy and material metabolism. Considering the serious side effects and weight regain associated with existing weight loss interventions, novel strategies are urgently needed for prevention and treatment of obesity. RESULTS: In the present study, we engineered Bacillus subtilis SCK6 to exhibited enhanced butyric acid production. Compared to the original Bacillus subtilis SCK6 strain, the genetically modified BsS-RS06550 strain had higher butyric acid production. The mice were randomly divided into four groups: a normal diet (C) group, a high-fat diet (HFD) group, an HFD + Bacillus subtilis SCK6 (HS) group and an HFD + BsS-RS06550 (HE) group. The results showed BsS-RS06550 decreased the body weight, body weight gain, and food intake of HFD mice. BsS-RS06550 had beneficial effects on blood glucose, insulin resistance and hepatic biochemistry. After the 14-week of experiment, fecal samples were collected for nontargeted liquid chromatography-mass spectrometry analysis to identify and quantify significant changes in metabolites. Sixteen potentially significant metabolites were screened, and BsS-RS06550 was shown to potentially regulate disorders in glutathione, methionine, tyrosine, phenylalanine, and purine metabolism and secondary bile acid biosynthesis. CONCLUSIONS: In this study, we successfully engineered Bacillus subtilis SCK6 to have enhanced butyric acid production. The results of this work revealed that the genetically modified live bacterium BsS-RS06550 showed potential anti-obesity effects, which may have been related to regulating the levels of metabolites associated with obesity. These results indicate that the use of BsS-RS06550 may be a promising strategy to attenuate obesity. [Image: see text] |
format | Online Article Text |
id | pubmed-7183672 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-71836722020-04-29 Engineered butyrate-producing bacteria prevents high fat diet-induced obesity in mice Bai, Liang Gao, Mengxue Cheng, Xiaoming Kang, Guangbo Cao, Xiaocang Huang, He Microb Cell Fact Research BACKGROUND: Obesity is a major problem worldwide and severely affects public safety. As a metabolite of gut microbiota, endogenous butyric acid participates in energy and material metabolism. Considering the serious side effects and weight regain associated with existing weight loss interventions, novel strategies are urgently needed for prevention and treatment of obesity. RESULTS: In the present study, we engineered Bacillus subtilis SCK6 to exhibited enhanced butyric acid production. Compared to the original Bacillus subtilis SCK6 strain, the genetically modified BsS-RS06550 strain had higher butyric acid production. The mice were randomly divided into four groups: a normal diet (C) group, a high-fat diet (HFD) group, an HFD + Bacillus subtilis SCK6 (HS) group and an HFD + BsS-RS06550 (HE) group. The results showed BsS-RS06550 decreased the body weight, body weight gain, and food intake of HFD mice. BsS-RS06550 had beneficial effects on blood glucose, insulin resistance and hepatic biochemistry. After the 14-week of experiment, fecal samples were collected for nontargeted liquid chromatography-mass spectrometry analysis to identify and quantify significant changes in metabolites. Sixteen potentially significant metabolites were screened, and BsS-RS06550 was shown to potentially regulate disorders in glutathione, methionine, tyrosine, phenylalanine, and purine metabolism and secondary bile acid biosynthesis. CONCLUSIONS: In this study, we successfully engineered Bacillus subtilis SCK6 to have enhanced butyric acid production. The results of this work revealed that the genetically modified live bacterium BsS-RS06550 showed potential anti-obesity effects, which may have been related to regulating the levels of metabolites associated with obesity. These results indicate that the use of BsS-RS06550 may be a promising strategy to attenuate obesity. [Image: see text] BioMed Central 2020-04-25 /pmc/articles/PMC7183672/ /pubmed/32334588 http://dx.doi.org/10.1186/s12934-020-01350-z Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Bai, Liang Gao, Mengxue Cheng, Xiaoming Kang, Guangbo Cao, Xiaocang Huang, He Engineered butyrate-producing bacteria prevents high fat diet-induced obesity in mice |
title | Engineered butyrate-producing bacteria prevents high fat diet-induced obesity in mice |
title_full | Engineered butyrate-producing bacteria prevents high fat diet-induced obesity in mice |
title_fullStr | Engineered butyrate-producing bacteria prevents high fat diet-induced obesity in mice |
title_full_unstemmed | Engineered butyrate-producing bacteria prevents high fat diet-induced obesity in mice |
title_short | Engineered butyrate-producing bacteria prevents high fat diet-induced obesity in mice |
title_sort | engineered butyrate-producing bacteria prevents high fat diet-induced obesity in mice |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7183672/ https://www.ncbi.nlm.nih.gov/pubmed/32334588 http://dx.doi.org/10.1186/s12934-020-01350-z |
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