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Effective microtissue RNA extraction coupled with Smart-seq2 for reproducible and robust spatial transcriptome analysis
Spatial transcriptomics is useful for understanding the molecular organization of a tissue and providing insights into cellular function in a morphological context. In order to obtain reproducible results in spatial transcriptomics, we have to maintain tissue morphology and RNA molecule stability du...
Autores principales: | Yamazaki, Miki, Hosokawa, Masahito, Arikawa, Koji, Takahashi, Kiyofumi, Sakanashi, Chikako, Yoda, Takuya, Matsunaga, Hiroko, Takeyama, Haruko |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7184581/ https://www.ncbi.nlm.nih.gov/pubmed/32341421 http://dx.doi.org/10.1038/s41598-020-63495-6 |
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