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Effect of Tahiti lime (Citrus latifolia) juice on the Production of the PGF2α/PGE2 and Pro-Inflammatory Cytokines involved in Menstruation
Tahiti lemon juice (Citrus latifolia) (TLJ), as a natural source of flavonoids, has been used as an alternative to anti-inflammatory drugs for the treatment of dysmenorrhea and menstrual excessive bleeding, often associated with an imbalance of the prostaglandins (PG) levels. However, despite the po...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7184590/ https://www.ncbi.nlm.nih.gov/pubmed/32341436 http://dx.doi.org/10.1038/s41598-020-63477-8 |
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author | Robeldo, Thaiane Canzi, Edione Fatima de Andrade, Priscila Maria Santana, Jhonne Pedro Pedotte Teixeira, Felipe Roberti Spagnol, Valentine Maia, Beatriz Helena Lameiro Noronha Sales Carbol, Maristela Caneira, Erika Gonçalves Da Silva, Maria Fátima das Graças Fernandes Borra, Ricardo Carneiro |
author_facet | Robeldo, Thaiane Canzi, Edione Fatima de Andrade, Priscila Maria Santana, Jhonne Pedro Pedotte Teixeira, Felipe Roberti Spagnol, Valentine Maia, Beatriz Helena Lameiro Noronha Sales Carbol, Maristela Caneira, Erika Gonçalves Da Silva, Maria Fátima das Graças Fernandes Borra, Ricardo Carneiro |
author_sort | Robeldo, Thaiane |
collection | PubMed |
description | Tahiti lemon juice (Citrus latifolia) (TLJ), as a natural source of flavonoids, has been used as an alternative to anti-inflammatory drugs for the treatment of dysmenorrhea and menstrual excessive bleeding, often associated with an imbalance of the prostaglandins (PG) levels. However, despite the positive effects, the mechanisms that rule menstruation control are still unknown. Therefore, the objectives were to characterize the TLJ and analyze its effect on the production of PGF2α, PGE2 and pro-inflammatory cytokines involved inmenstruation. Flavonoids from TLJ were discriminated by UPLC-DAD-MS/MS (Qq-TOF) and the effects of TLJ were studied in vitro by quantification of the contraction of myoblasts in culture and PGF2α and PGE2 productions. Further, the systemic and menstrual fluid levels of PGF2α, PGE2, IL-1β, TNF-α, IL-6, AK1B1 and AK1C3 enzymes produced by women during the menstrual period were compared after exposition or not to TLJ or meloxicam. The results showed that TLJ induces an increase in the contraction of myoblasts and the PGF2α supernatant level. Regarding in vivo analysis, a higher concentration of PGF2α and an unaltered PGE2 level was also found in the menstrual blood of women treated with TLJ, in contrast with a lower level of PGE2 and PGF2α observed in the meloxicam group. Concerning cytokines, only menstrual TNF-α levels decrease after treatment with TLJ or meloxicam. In conclusion, TLJ may favor the control of menstruation events via a PGF2α mediated muscle contractile response. |
format | Online Article Text |
id | pubmed-7184590 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-71845902020-04-29 Effect of Tahiti lime (Citrus latifolia) juice on the Production of the PGF2α/PGE2 and Pro-Inflammatory Cytokines involved in Menstruation Robeldo, Thaiane Canzi, Edione Fatima de Andrade, Priscila Maria Santana, Jhonne Pedro Pedotte Teixeira, Felipe Roberti Spagnol, Valentine Maia, Beatriz Helena Lameiro Noronha Sales Carbol, Maristela Caneira, Erika Gonçalves Da Silva, Maria Fátima das Graças Fernandes Borra, Ricardo Carneiro Sci Rep Article Tahiti lemon juice (Citrus latifolia) (TLJ), as a natural source of flavonoids, has been used as an alternative to anti-inflammatory drugs for the treatment of dysmenorrhea and menstrual excessive bleeding, often associated with an imbalance of the prostaglandins (PG) levels. However, despite the positive effects, the mechanisms that rule menstruation control are still unknown. Therefore, the objectives were to characterize the TLJ and analyze its effect on the production of PGF2α, PGE2 and pro-inflammatory cytokines involved inmenstruation. Flavonoids from TLJ were discriminated by UPLC-DAD-MS/MS (Qq-TOF) and the effects of TLJ were studied in vitro by quantification of the contraction of myoblasts in culture and PGF2α and PGE2 productions. Further, the systemic and menstrual fluid levels of PGF2α, PGE2, IL-1β, TNF-α, IL-6, AK1B1 and AK1C3 enzymes produced by women during the menstrual period were compared after exposition or not to TLJ or meloxicam. The results showed that TLJ induces an increase in the contraction of myoblasts and the PGF2α supernatant level. Regarding in vivo analysis, a higher concentration of PGF2α and an unaltered PGE2 level was also found in the menstrual blood of women treated with TLJ, in contrast with a lower level of PGE2 and PGF2α observed in the meloxicam group. Concerning cytokines, only menstrual TNF-α levels decrease after treatment with TLJ or meloxicam. In conclusion, TLJ may favor the control of menstruation events via a PGF2α mediated muscle contractile response. Nature Publishing Group UK 2020-04-27 /pmc/articles/PMC7184590/ /pubmed/32341436 http://dx.doi.org/10.1038/s41598-020-63477-8 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Robeldo, Thaiane Canzi, Edione Fatima de Andrade, Priscila Maria Santana, Jhonne Pedro Pedotte Teixeira, Felipe Roberti Spagnol, Valentine Maia, Beatriz Helena Lameiro Noronha Sales Carbol, Maristela Caneira, Erika Gonçalves Da Silva, Maria Fátima das Graças Fernandes Borra, Ricardo Carneiro Effect of Tahiti lime (Citrus latifolia) juice on the Production of the PGF2α/PGE2 and Pro-Inflammatory Cytokines involved in Menstruation |
title | Effect of Tahiti lime (Citrus latifolia) juice on the Production of the PGF2α/PGE2 and Pro-Inflammatory Cytokines involved in Menstruation |
title_full | Effect of Tahiti lime (Citrus latifolia) juice on the Production of the PGF2α/PGE2 and Pro-Inflammatory Cytokines involved in Menstruation |
title_fullStr | Effect of Tahiti lime (Citrus latifolia) juice on the Production of the PGF2α/PGE2 and Pro-Inflammatory Cytokines involved in Menstruation |
title_full_unstemmed | Effect of Tahiti lime (Citrus latifolia) juice on the Production of the PGF2α/PGE2 and Pro-Inflammatory Cytokines involved in Menstruation |
title_short | Effect of Tahiti lime (Citrus latifolia) juice on the Production of the PGF2α/PGE2 and Pro-Inflammatory Cytokines involved in Menstruation |
title_sort | effect of tahiti lime (citrus latifolia) juice on the production of the pgf2α/pge2 and pro-inflammatory cytokines involved in menstruation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7184590/ https://www.ncbi.nlm.nih.gov/pubmed/32341436 http://dx.doi.org/10.1038/s41598-020-63477-8 |
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