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Repurposing a chemosensory macromolecular machine

How complex, multi-component macromolecular machines evolved remains poorly understood. Here we reveal the evolutionary origins of the chemosensory machinery that controls flagellar motility in Escherichia coli. We first identify ancestral forms still present in Vibrio cholerae, Pseudomonas aerugino...

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Autores principales: Ortega, Davi R., Yang, Wen, Subramanian, Poorna, Mann, Petra, Kjær, Andreas, Chen, Songye, Watts, Kylie J., Pirbadian, Sahand, Collins, David A., Kooger, Romain, Kalyuzhnaya, Marina G., Ringgaard, Simon, Briegel, Ariane, Jensen, Grant J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7184735/
https://www.ncbi.nlm.nih.gov/pubmed/32341341
http://dx.doi.org/10.1038/s41467-020-15736-5
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author Ortega, Davi R.
Yang, Wen
Subramanian, Poorna
Mann, Petra
Kjær, Andreas
Chen, Songye
Watts, Kylie J.
Pirbadian, Sahand
Collins, David A.
Kooger, Romain
Kalyuzhnaya, Marina G.
Ringgaard, Simon
Briegel, Ariane
Jensen, Grant J.
author_facet Ortega, Davi R.
Yang, Wen
Subramanian, Poorna
Mann, Petra
Kjær, Andreas
Chen, Songye
Watts, Kylie J.
Pirbadian, Sahand
Collins, David A.
Kooger, Romain
Kalyuzhnaya, Marina G.
Ringgaard, Simon
Briegel, Ariane
Jensen, Grant J.
author_sort Ortega, Davi R.
collection PubMed
description How complex, multi-component macromolecular machines evolved remains poorly understood. Here we reveal the evolutionary origins of the chemosensory machinery that controls flagellar motility in Escherichia coli. We first identify ancestral forms still present in Vibrio cholerae, Pseudomonas aeruginosa, Shewanella oneidensis and Methylomicrobium alcaliphilum, characterizing their structures by electron cryotomography and finding evidence that they function in a stress response pathway. Using bioinformatics, we trace the evolution of the system through γ-Proteobacteria, pinpointing key evolutionary events that led to the machine now seen in E. coli. Our results suggest that two ancient chemosensory systems with different inputs and outputs (F6 and F7) existed contemporaneously, with one (F7) ultimately taking over the inputs and outputs of the other (F6), which was subsequently lost.
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spelling pubmed-71847352020-04-30 Repurposing a chemosensory macromolecular machine Ortega, Davi R. Yang, Wen Subramanian, Poorna Mann, Petra Kjær, Andreas Chen, Songye Watts, Kylie J. Pirbadian, Sahand Collins, David A. Kooger, Romain Kalyuzhnaya, Marina G. Ringgaard, Simon Briegel, Ariane Jensen, Grant J. Nat Commun Article How complex, multi-component macromolecular machines evolved remains poorly understood. Here we reveal the evolutionary origins of the chemosensory machinery that controls flagellar motility in Escherichia coli. We first identify ancestral forms still present in Vibrio cholerae, Pseudomonas aeruginosa, Shewanella oneidensis and Methylomicrobium alcaliphilum, characterizing their structures by electron cryotomography and finding evidence that they function in a stress response pathway. Using bioinformatics, we trace the evolution of the system through γ-Proteobacteria, pinpointing key evolutionary events that led to the machine now seen in E. coli. Our results suggest that two ancient chemosensory systems with different inputs and outputs (F6 and F7) existed contemporaneously, with one (F7) ultimately taking over the inputs and outputs of the other (F6), which was subsequently lost. Nature Publishing Group UK 2020-04-27 /pmc/articles/PMC7184735/ /pubmed/32341341 http://dx.doi.org/10.1038/s41467-020-15736-5 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ortega, Davi R.
Yang, Wen
Subramanian, Poorna
Mann, Petra
Kjær, Andreas
Chen, Songye
Watts, Kylie J.
Pirbadian, Sahand
Collins, David A.
Kooger, Romain
Kalyuzhnaya, Marina G.
Ringgaard, Simon
Briegel, Ariane
Jensen, Grant J.
Repurposing a chemosensory macromolecular machine
title Repurposing a chemosensory macromolecular machine
title_full Repurposing a chemosensory macromolecular machine
title_fullStr Repurposing a chemosensory macromolecular machine
title_full_unstemmed Repurposing a chemosensory macromolecular machine
title_short Repurposing a chemosensory macromolecular machine
title_sort repurposing a chemosensory macromolecular machine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7184735/
https://www.ncbi.nlm.nih.gov/pubmed/32341341
http://dx.doi.org/10.1038/s41467-020-15736-5
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