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DNA N(6)-methyladenine modification in hypertension
DNA methylation has a role in the pathogenesis of essential hypertension. DNA N6-methyladenine (6mA) modification as a novel adenine methylation exists in human tissues, but whether it plays a role in hypertension development remains unclear. Here, we reported that the global 6mA DNA level in leukoc...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7185115/ https://www.ncbi.nlm.nih.gov/pubmed/32283543 http://dx.doi.org/10.18632/aging.103023 |
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author | Guo, Ye Pei, Yuqing Li, Kexin Cui, Wei Zhang, Donghong |
author_facet | Guo, Ye Pei, Yuqing Li, Kexin Cui, Wei Zhang, Donghong |
author_sort | Guo, Ye |
collection | PubMed |
description | DNA methylation has a role in the pathogenesis of essential hypertension. DNA N6-methyladenine (6mA) modification as a novel adenine methylation exists in human tissues, but whether it plays a role in hypertension development remains unclear. Here, we reported that the global 6mA DNA level in leukocytes was significantly reduced in patients with hypertension and was reversed with successful treatment. Age, systolic blood pressure, and serum total cholesterol and high-density lipoprotein levels were associated with decreased leukocyte 6mA DNA level. Elevated ALKBH1 (AlkB homolog 1), a demethylase of 6mA, level mediated this dynamic change in 6mA level in leukocytes and vascular smooth muscle cells in hypertension mouse and rat models. Knockdown of ALKBH1 suppressed angiotensin II-induced vascular smooth muscle phenotype transformation, proliferation and migration. ALKBH1-6mA directly and negatively regulated hypoxia inducible factor 1 α (HIF1α), which responded to angiotensin II-induced vascular remodeling. Collectively, our results demonstrate a potential epigenetic role for ALKBH1-6mA regulation in hypertension development, diagnosis and treatment. |
format | Online Article Text |
id | pubmed-7185115 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Impact Journals |
record_format | MEDLINE/PubMed |
spelling | pubmed-71851152020-05-01 DNA N(6)-methyladenine modification in hypertension Guo, Ye Pei, Yuqing Li, Kexin Cui, Wei Zhang, Donghong Aging (Albany NY) Research Paper DNA methylation has a role in the pathogenesis of essential hypertension. DNA N6-methyladenine (6mA) modification as a novel adenine methylation exists in human tissues, but whether it plays a role in hypertension development remains unclear. Here, we reported that the global 6mA DNA level in leukocytes was significantly reduced in patients with hypertension and was reversed with successful treatment. Age, systolic blood pressure, and serum total cholesterol and high-density lipoprotein levels were associated with decreased leukocyte 6mA DNA level. Elevated ALKBH1 (AlkB homolog 1), a demethylase of 6mA, level mediated this dynamic change in 6mA level in leukocytes and vascular smooth muscle cells in hypertension mouse and rat models. Knockdown of ALKBH1 suppressed angiotensin II-induced vascular smooth muscle phenotype transformation, proliferation and migration. ALKBH1-6mA directly and negatively regulated hypoxia inducible factor 1 α (HIF1α), which responded to angiotensin II-induced vascular remodeling. Collectively, our results demonstrate a potential epigenetic role for ALKBH1-6mA regulation in hypertension development, diagnosis and treatment. Impact Journals 2020-04-13 /pmc/articles/PMC7185115/ /pubmed/32283543 http://dx.doi.org/10.18632/aging.103023 Text en Copyright © 2020 Guo et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Guo, Ye Pei, Yuqing Li, Kexin Cui, Wei Zhang, Donghong DNA N(6)-methyladenine modification in hypertension |
title | DNA N(6)-methyladenine modification in hypertension |
title_full | DNA N(6)-methyladenine modification in hypertension |
title_fullStr | DNA N(6)-methyladenine modification in hypertension |
title_full_unstemmed | DNA N(6)-methyladenine modification in hypertension |
title_short | DNA N(6)-methyladenine modification in hypertension |
title_sort | dna n(6)-methyladenine modification in hypertension |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7185115/ https://www.ncbi.nlm.nih.gov/pubmed/32283543 http://dx.doi.org/10.18632/aging.103023 |
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