Characterization of human peripheral blood γδ T cells in patients with sepsis

In total, 30 cases of patients undergoing health check-ups with the diagnostic criteria of sepsis were included in the present study. The clinical data of each patient with sepsis were recorded at admission. In the present study, the association between the proportion of T cells in patients with sepsis and those in a healthy condition were observed. The expression of immunosuppressive molecules on the surface of Vδ1 T cells were examined, as well as studying the secretion of inflammatory cytokines in Vδ2 T cells, and the ability of the Vδ1 T cells to inhibit the secretory level of interferon-γ (IFN-γ) and the inflammatory function of Vδ2 T cells were monitored. The inhibition of proliferation of naïve CD4 T cells by Vδ1 T cells and inflammatory function of Vδ2 T cells were examined. The number of Vδ1 T cells in the peripheral blood of patients with sepsis was significantly increased compared with healthy controls (P<0.01); the proportion of Vδ2 T cells was opposite to that of Vδ1 T cells. The Sequential Organ Failure Assessment score, survival and survival time were positively associated with Vδ1 T cell ratio (P<0.05) and negatively correlated with Vδ2 T cells. The expression of cytotoxic T-lymphocyte protein 4 and T cell immunoglobulin and mucin domain-containing protein 3 on the surface of Vδ1 T cells in the peripheral blood of patients with sepsis was significantly increased compared with the healthy controls (P<0.01), and the levels of IFN-γ and tumor necrosis factor-α secreted by Vδ2 T cells were significantly decreased (P<0.01). The immunosuppressive function of Vδ1 T cells was significantly higher, and the function of Vδ2 T cells was significantly reduced (P<0.01). The phosphorylation level of Erk1/2 in Vδ2 T cells was significantly lower (P<0.01). The present results suggested that the imbalance and functional changes of different γδ T cell subtypes in the peripheral blood of patients with sepsis are associated with sepsis, and may be involved in sepsis progression.