Cargando…

Housing influences tissue cytokine levels and the fecal bacterial community structure in rats

Immune measures and the fecal bacterial community were examined in female Biobreeding rats housed in wire bottom cages (wire) or in solid bottom cages containing hardwood chips (bedding). Housing did not affect food intake, weight gain, fecal output or fibre content, serum liver enzymes, or spleen a...

Descripción completa

Detalles Bibliográficos
Autores principales: Shastri, Padmaja, Green, Judy, Matias, Fernando, Kalmokoff, Martin L., Green-Johnson, Julia M., Brooks, Stephen P.J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Ltd. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7185516/
https://www.ncbi.nlm.nih.gov/pubmed/32362938
http://dx.doi.org/10.1016/j.jff.2017.10.021
_version_ 1783526772480933888
author Shastri, Padmaja
Green, Judy
Matias, Fernando
Kalmokoff, Martin L.
Green-Johnson, Julia M.
Brooks, Stephen P.J.
author_facet Shastri, Padmaja
Green, Judy
Matias, Fernando
Kalmokoff, Martin L.
Green-Johnson, Julia M.
Brooks, Stephen P.J.
author_sort Shastri, Padmaja
collection PubMed
description Immune measures and the fecal bacterial community were examined in female Biobreeding rats housed in wire bottom cages (wire) or in solid bottom cages containing hardwood chips (bedding). Housing did not affect food intake, weight gain, fecal output or fibre content, serum liver enzymes, or spleen and mesenteric lymph node immune cell populations. Bedding-housed rat feces were enriched in phylotypes aligning within the phylum Firmicutes (families Lactobacillaceae and Erysipelotrichaceae) and had a 2-fold lower content of phylotypes aligning within the phylum Bacteroidetes. Feces from bedding-housed rats also contained significantly more acetic acid and less propionic, isobutyric, valeric and isovaleric acids than those housed on wire. Bedding-housed rats had significantly higher splenic concentrations of interleukin-4 (P < 0.001). These results demonstrate that bedding can indirectly influence systemic and mucosal immune measures, potentially adding additional complexities and confounding results to nutrition studies investigating the health effects of dietary fibres.
format Online
Article
Text
id pubmed-7185516
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Published by Elsevier Ltd.
record_format MEDLINE/PubMed
spelling pubmed-71855162020-04-28 Housing influences tissue cytokine levels and the fecal bacterial community structure in rats Shastri, Padmaja Green, Judy Matias, Fernando Kalmokoff, Martin L. Green-Johnson, Julia M. Brooks, Stephen P.J. J Funct Foods Article Immune measures and the fecal bacterial community were examined in female Biobreeding rats housed in wire bottom cages (wire) or in solid bottom cages containing hardwood chips (bedding). Housing did not affect food intake, weight gain, fecal output or fibre content, serum liver enzymes, or spleen and mesenteric lymph node immune cell populations. Bedding-housed rat feces were enriched in phylotypes aligning within the phylum Firmicutes (families Lactobacillaceae and Erysipelotrichaceae) and had a 2-fold lower content of phylotypes aligning within the phylum Bacteroidetes. Feces from bedding-housed rats also contained significantly more acetic acid and less propionic, isobutyric, valeric and isovaleric acids than those housed on wire. Bedding-housed rats had significantly higher splenic concentrations of interleukin-4 (P < 0.001). These results demonstrate that bedding can indirectly influence systemic and mucosal immune measures, potentially adding additional complexities and confounding results to nutrition studies investigating the health effects of dietary fibres. Published by Elsevier Ltd. 2017-12 2017-11-02 /pmc/articles/PMC7185516/ /pubmed/32362938 http://dx.doi.org/10.1016/j.jff.2017.10.021 Text en Crown Copyright © 2017 Published by Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Shastri, Padmaja
Green, Judy
Matias, Fernando
Kalmokoff, Martin L.
Green-Johnson, Julia M.
Brooks, Stephen P.J.
Housing influences tissue cytokine levels and the fecal bacterial community structure in rats
title Housing influences tissue cytokine levels and the fecal bacterial community structure in rats
title_full Housing influences tissue cytokine levels and the fecal bacterial community structure in rats
title_fullStr Housing influences tissue cytokine levels and the fecal bacterial community structure in rats
title_full_unstemmed Housing influences tissue cytokine levels and the fecal bacterial community structure in rats
title_short Housing influences tissue cytokine levels and the fecal bacterial community structure in rats
title_sort housing influences tissue cytokine levels and the fecal bacterial community structure in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7185516/
https://www.ncbi.nlm.nih.gov/pubmed/32362938
http://dx.doi.org/10.1016/j.jff.2017.10.021
work_keys_str_mv AT shastripadmaja housinginfluencestissuecytokinelevelsandthefecalbacterialcommunitystructureinrats
AT greenjudy housinginfluencestissuecytokinelevelsandthefecalbacterialcommunitystructureinrats
AT matiasfernando housinginfluencestissuecytokinelevelsandthefecalbacterialcommunitystructureinrats
AT kalmokoffmartinl housinginfluencestissuecytokinelevelsandthefecalbacterialcommunitystructureinrats
AT greenjohnsonjuliam housinginfluencestissuecytokinelevelsandthefecalbacterialcommunitystructureinrats
AT brooksstephenpj housinginfluencestissuecytokinelevelsandthefecalbacterialcommunitystructureinrats