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One-tube fluorogenic reverse transcription-polymerase chain reaction for the quantitation of feline coronaviruses

A one-tube reverse transcription-polymerase chain reaction (RT-PCR) for absolute feline coronavirus (FCoV) quantitation was developed. The assay is based on the 5′ nuclease activity of the Thermus flavus (Tfl) polymerase and a fluorogenic probe which generates fluorescence when it is cleaved. The fl...

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Detalles Bibliográficos
Autores principales:	Gut, Marco, M. Leutenegger, Christian, B. Huder, Jon, C. Pedersen, Niels, Lutz, Hans
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier Science B.V. 1999
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7185542/ https://www.ncbi.nlm.nih.gov/pubmed/10029323 http://dx.doi.org/10.1016/S0166-0934(98)00129-3

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