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B cell characterization and reactivity analysis in multiple sclerosis
B cells are one of the key players in the pathogenesis of multiple sclerosis (MS). The peripheral B cell distributions are similar in healthy persons and MS patients. In healthy controls, B cells are rarely present in the cerebrospinal fluid (CSF) while in MS patients, a clonally expanded B cell pop...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7185554/ https://www.ncbi.nlm.nih.gov/pubmed/19239929 http://dx.doi.org/10.1016/j.autrev.2009.02.030 |
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author | Fraussen, J. Vrolix, K. Martinez-Martinez, P. Losen, M. De Baets, M.H. Stinissen, P. Somers, V. |
author_facet | Fraussen, J. Vrolix, K. Martinez-Martinez, P. Losen, M. De Baets, M.H. Stinissen, P. Somers, V. |
author_sort | Fraussen, J. |
collection | PubMed |
description | B cells are one of the key players in the pathogenesis of multiple sclerosis (MS). The peripheral B cell distributions are similar in healthy persons and MS patients. In healthy controls, B cells are rarely present in the cerebrospinal fluid (CSF) while in MS patients, a clonally expanded B cell population is detected. This consists of memory B cells, centroblasts and antibody-secreting plasma blasts and plasma cells that are responsible for intrathecal immunoglobulin G production and oligoclonal band formation in more than 90% of MS patients. Unfortunately, the targets of the autoreactive B cells and antibodies remain largely unknown. Various candidate antigens have been identified but often their involvement in the disease process is still unclear. Most studies characterizing these target antigens examined autoantibodies by analyzing sera or CSF of MS patients. An alternative approach is focusing on the clonally expanded B cells. In this way B cells directed against myelin, astroglia and axons have been denoted in MS patients. B cell immortalization, that is based on the antibody-producing potential of Epstein–Barr virus (EBV) transformed B cells, can be used to expand B cells from MS patients for the production of antibodies, that ultimately can be analysed for target identification. |
format | Online Article Text |
id | pubmed-7185554 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71855542020-04-28 B cell characterization and reactivity analysis in multiple sclerosis Fraussen, J. Vrolix, K. Martinez-Martinez, P. Losen, M. De Baets, M.H. Stinissen, P. Somers, V. Autoimmun Rev Article B cells are one of the key players in the pathogenesis of multiple sclerosis (MS). The peripheral B cell distributions are similar in healthy persons and MS patients. In healthy controls, B cells are rarely present in the cerebrospinal fluid (CSF) while in MS patients, a clonally expanded B cell population is detected. This consists of memory B cells, centroblasts and antibody-secreting plasma blasts and plasma cells that are responsible for intrathecal immunoglobulin G production and oligoclonal band formation in more than 90% of MS patients. Unfortunately, the targets of the autoreactive B cells and antibodies remain largely unknown. Various candidate antigens have been identified but often their involvement in the disease process is still unclear. Most studies characterizing these target antigens examined autoantibodies by analyzing sera or CSF of MS patients. An alternative approach is focusing on the clonally expanded B cells. In this way B cells directed against myelin, astroglia and axons have been denoted in MS patients. B cell immortalization, that is based on the antibody-producing potential of Epstein–Barr virus (EBV) transformed B cells, can be used to expand B cells from MS patients for the production of antibodies, that ultimately can be analysed for target identification. Elsevier B.V. 2009-07 2009-02-21 /pmc/articles/PMC7185554/ /pubmed/19239929 http://dx.doi.org/10.1016/j.autrev.2009.02.030 Text en Copyright © 2009 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Fraussen, J. Vrolix, K. Martinez-Martinez, P. Losen, M. De Baets, M.H. Stinissen, P. Somers, V. B cell characterization and reactivity analysis in multiple sclerosis |
title | B cell characterization and reactivity analysis in multiple sclerosis |
title_full | B cell characterization and reactivity analysis in multiple sclerosis |
title_fullStr | B cell characterization and reactivity analysis in multiple sclerosis |
title_full_unstemmed | B cell characterization and reactivity analysis in multiple sclerosis |
title_short | B cell characterization and reactivity analysis in multiple sclerosis |
title_sort | b cell characterization and reactivity analysis in multiple sclerosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7185554/ https://www.ncbi.nlm.nih.gov/pubmed/19239929 http://dx.doi.org/10.1016/j.autrev.2009.02.030 |
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