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Diagnosis and epidemiological studies of human metapneumovirus using real-time PCR
BACKGROUND: Human metapneumovirus (hMPV) is prevalent in children, the elderly and immunocompromised individuals, but available epidemiological data is limited. OBJECTIVES: (1) To develop and validate a real-time PCR method for hMPV diagnosis. (2) To determine the percentage of hMPV in respiratory s...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7185675/ https://www.ncbi.nlm.nih.gov/pubmed/17870660 http://dx.doi.org/10.1016/j.jcv.2007.08.004 |
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author | Pabbaraju, Kanti Wong, Sallene McMillan, Thomas Lee, Bonita E. Fox, Julie D. |
author_facet | Pabbaraju, Kanti Wong, Sallene McMillan, Thomas Lee, Bonita E. Fox, Julie D. |
author_sort | Pabbaraju, Kanti |
collection | PubMed |
description | BACKGROUND: Human metapneumovirus (hMPV) is prevalent in children, the elderly and immunocompromised individuals, but available epidemiological data is limited. OBJECTIVES: (1) To develop and validate a real-time PCR method for hMPV diagnosis. (2) To determine the percentage of hMPV in respiratory specimens from the community and its association with outbreaks in our geographic area. (3) To provide epidemiological data in terms of age distribution, seasonality and co-infections. STUDY DESIGN: A real-time PCR assay was designed for detection of hMPV lineages A and B. Prospective testing for hMPV over a 22-month period was then undertaken. RESULTS: The real-time PCR was sensitive and specific for detection of both lineages of hMPV. hMPV was detected in 9.5% (n = 8239) of the specimens and 25% of the outbreaks (n = 100) tested. The hMPV-positive patients ranged in age from 18 days to 99 years with a median age of 24 months. The number of positive samples peaked during the winter months of December, January and February. A high rate of co-infections was noted in the samples tested. CONCLUSIONS: hMPV is common in the community and is associated with outbreaks. Including hMPV in routine testing improves etiological diagnosis of acute respiratory infections. |
format | Online Article Text |
id | pubmed-7185675 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71856752020-04-28 Diagnosis and epidemiological studies of human metapneumovirus using real-time PCR Pabbaraju, Kanti Wong, Sallene McMillan, Thomas Lee, Bonita E. Fox, Julie D. J Clin Virol Article BACKGROUND: Human metapneumovirus (hMPV) is prevalent in children, the elderly and immunocompromised individuals, but available epidemiological data is limited. OBJECTIVES: (1) To develop and validate a real-time PCR method for hMPV diagnosis. (2) To determine the percentage of hMPV in respiratory specimens from the community and its association with outbreaks in our geographic area. (3) To provide epidemiological data in terms of age distribution, seasonality and co-infections. STUDY DESIGN: A real-time PCR assay was designed for detection of hMPV lineages A and B. Prospective testing for hMPV over a 22-month period was then undertaken. RESULTS: The real-time PCR was sensitive and specific for detection of both lineages of hMPV. hMPV was detected in 9.5% (n = 8239) of the specimens and 25% of the outbreaks (n = 100) tested. The hMPV-positive patients ranged in age from 18 days to 99 years with a median age of 24 months. The number of positive samples peaked during the winter months of December, January and February. A high rate of co-infections was noted in the samples tested. CONCLUSIONS: hMPV is common in the community and is associated with outbreaks. Including hMPV in routine testing improves etiological diagnosis of acute respiratory infections. Elsevier B.V. 2007-11 2007-09-17 /pmc/articles/PMC7185675/ /pubmed/17870660 http://dx.doi.org/10.1016/j.jcv.2007.08.004 Text en Copyright © 2007 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Pabbaraju, Kanti Wong, Sallene McMillan, Thomas Lee, Bonita E. Fox, Julie D. Diagnosis and epidemiological studies of human metapneumovirus using real-time PCR |
title | Diagnosis and epidemiological studies of human metapneumovirus using real-time PCR |
title_full | Diagnosis and epidemiological studies of human metapneumovirus using real-time PCR |
title_fullStr | Diagnosis and epidemiological studies of human metapneumovirus using real-time PCR |
title_full_unstemmed | Diagnosis and epidemiological studies of human metapneumovirus using real-time PCR |
title_short | Diagnosis and epidemiological studies of human metapneumovirus using real-time PCR |
title_sort | diagnosis and epidemiological studies of human metapneumovirus using real-time pcr |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7185675/ https://www.ncbi.nlm.nih.gov/pubmed/17870660 http://dx.doi.org/10.1016/j.jcv.2007.08.004 |
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