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Dissection of Influenza Infection In Vivo by Single-Cell RNA Sequencing

The influenza virus is a major cause of morbidity and mortality worldwide. Yet, both the impact of intracellular viral replication and the variation in host response across different cell types remain uncharacterized. Here we used single-cell RNA sequencing to investigate the heterogeneity in the re...

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Autores principales: Steuerman, Yael, Cohen, Merav, Peshes-Yaloz, Naama, Valadarsky, Liran, Cohn, Ofir, David, Eyal, Frishberg, Amit, Mayo, Lior, Bacharach, Eran, Amit, Ido, Gat-Viks, Irit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7185763/
https://www.ncbi.nlm.nih.gov/pubmed/29886109
http://dx.doi.org/10.1016/j.cels.2018.05.008
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author Steuerman, Yael
Cohen, Merav
Peshes-Yaloz, Naama
Valadarsky, Liran
Cohn, Ofir
David, Eyal
Frishberg, Amit
Mayo, Lior
Bacharach, Eran
Amit, Ido
Gat-Viks, Irit
author_facet Steuerman, Yael
Cohen, Merav
Peshes-Yaloz, Naama
Valadarsky, Liran
Cohn, Ofir
David, Eyal
Frishberg, Amit
Mayo, Lior
Bacharach, Eran
Amit, Ido
Gat-Viks, Irit
author_sort Steuerman, Yael
collection PubMed
description The influenza virus is a major cause of morbidity and mortality worldwide. Yet, both the impact of intracellular viral replication and the variation in host response across different cell types remain uncharacterized. Here we used single-cell RNA sequencing to investigate the heterogeneity in the response of lung tissue cells to in vivo influenza infection. Analysis of viral and host transcriptomes in the same single cell enabled us to resolve the cellular heterogeneity of bystander (exposed but uninfected) as compared with infected cells. We reveal that all major immune and non-immune cell types manifest substantial fractions of infected cells, albeit at low viral transcriptome loads relative to epithelial cells. We show that all cell types respond primarily with a robust generic transcriptional response, and we demonstrate novel markers specific for influenza-infected as opposed to bystander cells. These findings open new avenues for targeted therapy aimed exclusively at infected cells.
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spelling pubmed-71857632020-04-28 Dissection of Influenza Infection In Vivo by Single-Cell RNA Sequencing Steuerman, Yael Cohen, Merav Peshes-Yaloz, Naama Valadarsky, Liran Cohn, Ofir David, Eyal Frishberg, Amit Mayo, Lior Bacharach, Eran Amit, Ido Gat-Viks, Irit Cell Syst Article The influenza virus is a major cause of morbidity and mortality worldwide. Yet, both the impact of intracellular viral replication and the variation in host response across different cell types remain uncharacterized. Here we used single-cell RNA sequencing to investigate the heterogeneity in the response of lung tissue cells to in vivo influenza infection. Analysis of viral and host transcriptomes in the same single cell enabled us to resolve the cellular heterogeneity of bystander (exposed but uninfected) as compared with infected cells. We reveal that all major immune and non-immune cell types manifest substantial fractions of infected cells, albeit at low viral transcriptome loads relative to epithelial cells. We show that all cell types respond primarily with a robust generic transcriptional response, and we demonstrate novel markers specific for influenza-infected as opposed to bystander cells. These findings open new avenues for targeted therapy aimed exclusively at infected cells. Elsevier Inc. 2018-06-27 2018-06-06 /pmc/articles/PMC7185763/ /pubmed/29886109 http://dx.doi.org/10.1016/j.cels.2018.05.008 Text en © 2018 Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Steuerman, Yael
Cohen, Merav
Peshes-Yaloz, Naama
Valadarsky, Liran
Cohn, Ofir
David, Eyal
Frishberg, Amit
Mayo, Lior
Bacharach, Eran
Amit, Ido
Gat-Viks, Irit
Dissection of Influenza Infection In Vivo by Single-Cell RNA Sequencing
title Dissection of Influenza Infection In Vivo by Single-Cell RNA Sequencing
title_full Dissection of Influenza Infection In Vivo by Single-Cell RNA Sequencing
title_fullStr Dissection of Influenza Infection In Vivo by Single-Cell RNA Sequencing
title_full_unstemmed Dissection of Influenza Infection In Vivo by Single-Cell RNA Sequencing
title_short Dissection of Influenza Infection In Vivo by Single-Cell RNA Sequencing
title_sort dissection of influenza infection in vivo by single-cell rna sequencing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7185763/
https://www.ncbi.nlm.nih.gov/pubmed/29886109
http://dx.doi.org/10.1016/j.cels.2018.05.008
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