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The use of a multiplex real-time PCR assay for diagnosing acute respiratory viral infections in children attending an emergency unit
BACKGROUND: The use of a multiplex molecular technique to identify the etiological pathogen of respiratory viral infections might be a support as clinical signs are not characteristic. OBJECTIVES: The aim of the study was to evaluate a multiplex molecular real-time assay for the routine diagnosis of...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7185773/ https://www.ncbi.nlm.nih.gov/pubmed/25223920 http://dx.doi.org/10.1016/j.jcv.2014.08.023 |
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author | Mengelle, C. Mansuy, J.M. Pierre, A. Claudet, I. Grouteau, E. Micheau, P. Sauné, K. Izopet, J. |
author_facet | Mengelle, C. Mansuy, J.M. Pierre, A. Claudet, I. Grouteau, E. Micheau, P. Sauné, K. Izopet, J. |
author_sort | Mengelle, C. |
collection | PubMed |
description | BACKGROUND: The use of a multiplex molecular technique to identify the etiological pathogen of respiratory viral infections might be a support as clinical signs are not characteristic. OBJECTIVES: The aim of the study was to evaluate a multiplex molecular real-time assay for the routine diagnosis of respiratory viruses, to analyze the symptoms associated with the pathogens detected and to determine the spread of virus during the period. STUDY DESIGN: Respiratory samples were collected from children presenting with respiratory symptoms and attending the emergency unit during the 2010–2011 winter seasons. Samples were tested with the multiplex RespiFinder(®) 15 assay (PathoFinder™) which potentially detects 15 viruses. RESULTS: 857 (88.7%) of the 966 samples collected from 914 children were positive for one (683 samples) or multiple viruses (174 samples). The most prevalent were the respiratory syncytial virus (39.5%) and the rhinovirus (24.4%). Influenza viruses were detected in 139 (14.4%) samples. Adenovirus was detected in 93 (9.6%) samples, coronaviruses in 88 (9.1%), metapneumovirus in 51 (5.3%) and parainfluenzae in 47 (4.9%). Rhinovirus (40%) was the most prevalent pathogen in upper respiratory tract infections while respiratory syncytial virus (49.9%) was the most prevalent in lower respiratory tract infections. Co-infections were associated with severe respiratory symptoms. CONCLUSION: The multiplex assay detected clinically important viruses in a single genomic test and thus will be useful for detecting several viruses causing respiratory tract disorders. |
format | Online Article Text |
id | pubmed-7185773 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71857732020-04-28 The use of a multiplex real-time PCR assay for diagnosing acute respiratory viral infections in children attending an emergency unit Mengelle, C. Mansuy, J.M. Pierre, A. Claudet, I. Grouteau, E. Micheau, P. Sauné, K. Izopet, J. J Clin Virol Article BACKGROUND: The use of a multiplex molecular technique to identify the etiological pathogen of respiratory viral infections might be a support as clinical signs are not characteristic. OBJECTIVES: The aim of the study was to evaluate a multiplex molecular real-time assay for the routine diagnosis of respiratory viruses, to analyze the symptoms associated with the pathogens detected and to determine the spread of virus during the period. STUDY DESIGN: Respiratory samples were collected from children presenting with respiratory symptoms and attending the emergency unit during the 2010–2011 winter seasons. Samples were tested with the multiplex RespiFinder(®) 15 assay (PathoFinder™) which potentially detects 15 viruses. RESULTS: 857 (88.7%) of the 966 samples collected from 914 children were positive for one (683 samples) or multiple viruses (174 samples). The most prevalent were the respiratory syncytial virus (39.5%) and the rhinovirus (24.4%). Influenza viruses were detected in 139 (14.4%) samples. Adenovirus was detected in 93 (9.6%) samples, coronaviruses in 88 (9.1%), metapneumovirus in 51 (5.3%) and parainfluenzae in 47 (4.9%). Rhinovirus (40%) was the most prevalent pathogen in upper respiratory tract infections while respiratory syncytial virus (49.9%) was the most prevalent in lower respiratory tract infections. Co-infections were associated with severe respiratory symptoms. CONCLUSION: The multiplex assay detected clinically important viruses in a single genomic test and thus will be useful for detecting several viruses causing respiratory tract disorders. Elsevier B.V. 2014-11 2014-09-03 /pmc/articles/PMC7185773/ /pubmed/25223920 http://dx.doi.org/10.1016/j.jcv.2014.08.023 Text en Copyright © 2014 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Mengelle, C. Mansuy, J.M. Pierre, A. Claudet, I. Grouteau, E. Micheau, P. Sauné, K. Izopet, J. The use of a multiplex real-time PCR assay for diagnosing acute respiratory viral infections in children attending an emergency unit |
title | The use of a multiplex real-time PCR assay for diagnosing acute respiratory viral infections in children attending an emergency unit |
title_full | The use of a multiplex real-time PCR assay for diagnosing acute respiratory viral infections in children attending an emergency unit |
title_fullStr | The use of a multiplex real-time PCR assay for diagnosing acute respiratory viral infections in children attending an emergency unit |
title_full_unstemmed | The use of a multiplex real-time PCR assay for diagnosing acute respiratory viral infections in children attending an emergency unit |
title_short | The use of a multiplex real-time PCR assay for diagnosing acute respiratory viral infections in children attending an emergency unit |
title_sort | use of a multiplex real-time pcr assay for diagnosing acute respiratory viral infections in children attending an emergency unit |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7185773/ https://www.ncbi.nlm.nih.gov/pubmed/25223920 http://dx.doi.org/10.1016/j.jcv.2014.08.023 |
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