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Catching a DUB in the act: novel ubiquitin-based active site directed probes
Protein ubiquitylation is an important regulator of protein function, localization and half-life. It plays a key role in most cellular processes including immune signaling. Deregulation of this process is a major causative factor for many diseases. A major advancement in the identification and chara...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Ltd.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7185813/ https://www.ncbi.nlm.nih.gov/pubmed/25461387 http://dx.doi.org/10.1016/j.cbpa.2014.10.005 |
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author | Ekkebus, Reggy Flierman, Dennis Geurink, Paul P Ovaa, Huib |
author_facet | Ekkebus, Reggy Flierman, Dennis Geurink, Paul P Ovaa, Huib |
author_sort | Ekkebus, Reggy |
collection | PubMed |
description | Protein ubiquitylation is an important regulator of protein function, localization and half-life. It plays a key role in most cellular processes including immune signaling. Deregulation of this process is a major causative factor for many diseases. A major advancement in the identification and characterization of the enzymes that remove ubiquitin, deubiquitylases (DUBs) was made by the development of activity-based probes (ABPs). Recent advances in chemical protein synthesis and ligation methodology has yielded novel reagents for use in ubiquitylation research. We describe recent advances and discuss future directions in reagent development for studying DUBs. |
format | Online Article Text |
id | pubmed-7185813 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Elsevier Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71858132020-04-28 Catching a DUB in the act: novel ubiquitin-based active site directed probes Ekkebus, Reggy Flierman, Dennis Geurink, Paul P Ovaa, Huib Curr Opin Chem Biol Article Protein ubiquitylation is an important regulator of protein function, localization and half-life. It plays a key role in most cellular processes including immune signaling. Deregulation of this process is a major causative factor for many diseases. A major advancement in the identification and characterization of the enzymes that remove ubiquitin, deubiquitylases (DUBs) was made by the development of activity-based probes (ABPs). Recent advances in chemical protein synthesis and ligation methodology has yielded novel reagents for use in ubiquitylation research. We describe recent advances and discuss future directions in reagent development for studying DUBs. Elsevier Ltd. 2014-12 2014-11-03 /pmc/articles/PMC7185813/ /pubmed/25461387 http://dx.doi.org/10.1016/j.cbpa.2014.10.005 Text en Copyright © 2014 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Ekkebus, Reggy Flierman, Dennis Geurink, Paul P Ovaa, Huib Catching a DUB in the act: novel ubiquitin-based active site directed probes |
title | Catching a DUB in the act: novel ubiquitin-based active site directed probes |
title_full | Catching a DUB in the act: novel ubiquitin-based active site directed probes |
title_fullStr | Catching a DUB in the act: novel ubiquitin-based active site directed probes |
title_full_unstemmed | Catching a DUB in the act: novel ubiquitin-based active site directed probes |
title_short | Catching a DUB in the act: novel ubiquitin-based active site directed probes |
title_sort | catching a dub in the act: novel ubiquitin-based active site directed probes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7185813/ https://www.ncbi.nlm.nih.gov/pubmed/25461387 http://dx.doi.org/10.1016/j.cbpa.2014.10.005 |
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