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The role of Candida albicans in root caries biofilms: an RNA-seq analysis
OBJECTIVE: This study sought to analyze the gene expression of Candida albicans in sound root surface and root caries lesions, exploring its role in root caries pathogenesis. METHODOLOGY: The differential gene expression of C. albicans and the specific genes related to cariogenic traits were studied...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Faculdade De Odontologia De Bauru - USP
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7185980/ https://www.ncbi.nlm.nih.gov/pubmed/32348446 http://dx.doi.org/10.1590/1678-7757-2019-0578 |
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author | EV, Laís Daniela DAMÉ-TEIXEIRA, Nailê DO, Thuy MALTZ, Marisa PAROLO, Clarissa Cavalcanti Fatturi |
author_facet | EV, Laís Daniela DAMÉ-TEIXEIRA, Nailê DO, Thuy MALTZ, Marisa PAROLO, Clarissa Cavalcanti Fatturi |
author_sort | EV, Laís Daniela |
collection | PubMed |
description | OBJECTIVE: This study sought to analyze the gene expression of Candida albicans in sound root surface and root caries lesions, exploring its role in root caries pathogenesis. METHODOLOGY: The differential gene expression of C. albicans and the specific genes related to cariogenic traits were studied in association with samples of biofilm collected from exposed sound root surface (SRS, n=10) and from biofilm and carious dentin of active root carious lesions (RC, n=9). The total microbial RNA was extracted, and the cDNA libraries were prepared and sequenced on the Illumina Hi-Seq2500. Unique reads were mapped to 163 oral microbial reference genomes including two chromosomes of C. albicans SC5314 (14,217 genes). The putative presence of C. albicans was estimated (sum of reads/total number of genes≥1) in each sample. Count data were normalized (using the DESeq method package) to analyze differential gene expression (using the DESeq2R package) applying the Benjamini-Hochberg correction (FDR<0.05). RESULTS: Two genes (CaO19.610, FDR=0.009; CaO19.2506, FDR=0.018) were up-regulated on SRS, and their functions are related to biofilm formation. Seven genes ( UTP20 , FDR=0.018; ITR1 , FDR=0.036; DHN6 , FDR=0.046; CaO19.7197 , FDR=0.046; CaO19.7838 , FDR=0.046; STT4 , FDR=0.046; GUT1 , FDR=0.046) were up-regulated on RC and their functions are related to metabolic activity, sugar transport, stress tolerance, invasion and pH regulation. The use of alternative carbon sources, including lactate, and the ability to form hypha may be a unique trait of C. albicans influencing biofilm virulence. CONCLUSIONS: C. albicans is metabolically active in SRS and RC biofilm, with different roles in health and disease. |
format | Online Article Text |
id | pubmed-7185980 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Faculdade De Odontologia De Bauru - USP |
record_format | MEDLINE/PubMed |
spelling | pubmed-71859802020-05-04 The role of Candida albicans in root caries biofilms: an RNA-seq analysis EV, Laís Daniela DAMÉ-TEIXEIRA, Nailê DO, Thuy MALTZ, Marisa PAROLO, Clarissa Cavalcanti Fatturi J Appl Oral Sci Original Article OBJECTIVE: This study sought to analyze the gene expression of Candida albicans in sound root surface and root caries lesions, exploring its role in root caries pathogenesis. METHODOLOGY: The differential gene expression of C. albicans and the specific genes related to cariogenic traits were studied in association with samples of biofilm collected from exposed sound root surface (SRS, n=10) and from biofilm and carious dentin of active root carious lesions (RC, n=9). The total microbial RNA was extracted, and the cDNA libraries were prepared and sequenced on the Illumina Hi-Seq2500. Unique reads were mapped to 163 oral microbial reference genomes including two chromosomes of C. albicans SC5314 (14,217 genes). The putative presence of C. albicans was estimated (sum of reads/total number of genes≥1) in each sample. Count data were normalized (using the DESeq method package) to analyze differential gene expression (using the DESeq2R package) applying the Benjamini-Hochberg correction (FDR<0.05). RESULTS: Two genes (CaO19.610, FDR=0.009; CaO19.2506, FDR=0.018) were up-regulated on SRS, and their functions are related to biofilm formation. Seven genes ( UTP20 , FDR=0.018; ITR1 , FDR=0.036; DHN6 , FDR=0.046; CaO19.7197 , FDR=0.046; CaO19.7838 , FDR=0.046; STT4 , FDR=0.046; GUT1 , FDR=0.046) were up-regulated on RC and their functions are related to metabolic activity, sugar transport, stress tolerance, invasion and pH regulation. The use of alternative carbon sources, including lactate, and the ability to form hypha may be a unique trait of C. albicans influencing biofilm virulence. CONCLUSIONS: C. albicans is metabolically active in SRS and RC biofilm, with different roles in health and disease. Faculdade De Odontologia De Bauru - USP 2020-04-27 /pmc/articles/PMC7185980/ /pubmed/32348446 http://dx.doi.org/10.1590/1678-7757-2019-0578 Text en https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article EV, Laís Daniela DAMÉ-TEIXEIRA, Nailê DO, Thuy MALTZ, Marisa PAROLO, Clarissa Cavalcanti Fatturi The role of Candida albicans in root caries biofilms: an RNA-seq analysis |
title | The role of Candida albicans in root caries biofilms: an RNA-seq analysis |
title_full | The role of Candida albicans in root caries biofilms: an RNA-seq analysis |
title_fullStr | The role of Candida albicans in root caries biofilms: an RNA-seq analysis |
title_full_unstemmed | The role of Candida albicans in root caries biofilms: an RNA-seq analysis |
title_short | The role of Candida albicans in root caries biofilms: an RNA-seq analysis |
title_sort | role of candida albicans in root caries biofilms: an rna-seq analysis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7185980/ https://www.ncbi.nlm.nih.gov/pubmed/32348446 http://dx.doi.org/10.1590/1678-7757-2019-0578 |
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