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Dynamic ROS Production and Gene Expression of Heifers Blood Neutrophil in a Oligofructose Overload Model
Alimentary oligofructose (OF) overload can induce several diseases in cattle, such as ruminal acidosis, laminitis, and synovitis. The role of blood polymorphonuclear neutrophil (PMN) remains unclear during OF overload. The aim of this study was to investigate the dynamic changes in reactive oxygen s...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7186304/ https://www.ncbi.nlm.nih.gov/pubmed/32373641 http://dx.doi.org/10.3389/fvets.2020.00211 |
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author | Li, Shuaichen Ding, Jiafeng Jiang, Lihong Hayat, Muhammad Abid Song, Qiaozhi Li, Yuepeng Zhang, Xianhao Zhang, Jiantao |
author_facet | Li, Shuaichen Ding, Jiafeng Jiang, Lihong Hayat, Muhammad Abid Song, Qiaozhi Li, Yuepeng Zhang, Xianhao Zhang, Jiantao |
author_sort | Li, Shuaichen |
collection | PubMed |
description | Alimentary oligofructose (OF) overload can induce several diseases in cattle, such as ruminal acidosis, laminitis, and synovitis. The role of blood polymorphonuclear neutrophil (PMN) remains unclear during OF overload. The aim of this study was to investigate the dynamic changes in reactive oxygen species (ROS) production and the expression profile of genes in blood PMN in a model of OF overload. Twelve clinically healthy and non-pregnant Chinese Holstein heifers, aged between 18 and 26 mo, weighing 335–403 kg, BCS (5-point scale) ranges 2.7–3.3 were used for the experiments. OF heifers (n = 6) received 17 g/kg of BW oligofructose dissolved in 2 L/100 kg of BW tap water and the CON heifers (n = 6) received 2 L/100 kg of BW tap water. Blood PMN was isolated for each heifer 0, 6, 12, 18, 24, 36, 48, 60, and 72 h after administration. PMN was analyzed either by endogenous and phorbol myristate acetate (PMA)-induced ROS production or by quantitative real-time PCR. After 12 h, PMA-induced ROS production decreased, which was sustained until 48 h. The expressions of inflammation markers (IL1α, IL1β, IL6, IL10, TNFα, STAT3, TLR4, MMP9, and HP) and eicosanoids (ALOX5, ALOX5AP, and PLA2G4A) were upregulated. The expression of adhesion and migration (CXCR2, CXCL8, CD62L, ITGA4, ITGAM, and ITGB2) in OF heifers was increased compared with CON heifers. The expression of oxidative stress (SOD2 and S100A8) was upregulated, while SOD1 and MPO were downregulated. In metabolism and receptor genes, the expressions of GRα and INSR decreased after 12 h, while Fas increased until 6 h and then decreased at 18 h. The expression of LDHA and PANX1 did not show any differences after OF overload. These findings indicate that OF overload induced systemic activation of PMN, which provides a step toward a better understanding of the role of innate immune responses in response to oral OF administration. |
format | Online Article Text |
id | pubmed-7186304 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71863042020-05-05 Dynamic ROS Production and Gene Expression of Heifers Blood Neutrophil in a Oligofructose Overload Model Li, Shuaichen Ding, Jiafeng Jiang, Lihong Hayat, Muhammad Abid Song, Qiaozhi Li, Yuepeng Zhang, Xianhao Zhang, Jiantao Front Vet Sci Veterinary Science Alimentary oligofructose (OF) overload can induce several diseases in cattle, such as ruminal acidosis, laminitis, and synovitis. The role of blood polymorphonuclear neutrophil (PMN) remains unclear during OF overload. The aim of this study was to investigate the dynamic changes in reactive oxygen species (ROS) production and the expression profile of genes in blood PMN in a model of OF overload. Twelve clinically healthy and non-pregnant Chinese Holstein heifers, aged between 18 and 26 mo, weighing 335–403 kg, BCS (5-point scale) ranges 2.7–3.3 were used for the experiments. OF heifers (n = 6) received 17 g/kg of BW oligofructose dissolved in 2 L/100 kg of BW tap water and the CON heifers (n = 6) received 2 L/100 kg of BW tap water. Blood PMN was isolated for each heifer 0, 6, 12, 18, 24, 36, 48, 60, and 72 h after administration. PMN was analyzed either by endogenous and phorbol myristate acetate (PMA)-induced ROS production or by quantitative real-time PCR. After 12 h, PMA-induced ROS production decreased, which was sustained until 48 h. The expressions of inflammation markers (IL1α, IL1β, IL6, IL10, TNFα, STAT3, TLR4, MMP9, and HP) and eicosanoids (ALOX5, ALOX5AP, and PLA2G4A) were upregulated. The expression of adhesion and migration (CXCR2, CXCL8, CD62L, ITGA4, ITGAM, and ITGB2) in OF heifers was increased compared with CON heifers. The expression of oxidative stress (SOD2 and S100A8) was upregulated, while SOD1 and MPO were downregulated. In metabolism and receptor genes, the expressions of GRα and INSR decreased after 12 h, while Fas increased until 6 h and then decreased at 18 h. The expression of LDHA and PANX1 did not show any differences after OF overload. These findings indicate that OF overload induced systemic activation of PMN, which provides a step toward a better understanding of the role of innate immune responses in response to oral OF administration. Frontiers Media S.A. 2020-04-21 /pmc/articles/PMC7186304/ /pubmed/32373641 http://dx.doi.org/10.3389/fvets.2020.00211 Text en Copyright © 2020 Li, Ding, Jiang, Hayat, Song, Li, Zhang and Zhang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Li, Shuaichen Ding, Jiafeng Jiang, Lihong Hayat, Muhammad Abid Song, Qiaozhi Li, Yuepeng Zhang, Xianhao Zhang, Jiantao Dynamic ROS Production and Gene Expression of Heifers Blood Neutrophil in a Oligofructose Overload Model |
title | Dynamic ROS Production and Gene Expression of Heifers Blood Neutrophil in a Oligofructose Overload Model |
title_full | Dynamic ROS Production and Gene Expression of Heifers Blood Neutrophil in a Oligofructose Overload Model |
title_fullStr | Dynamic ROS Production and Gene Expression of Heifers Blood Neutrophil in a Oligofructose Overload Model |
title_full_unstemmed | Dynamic ROS Production and Gene Expression of Heifers Blood Neutrophil in a Oligofructose Overload Model |
title_short | Dynamic ROS Production and Gene Expression of Heifers Blood Neutrophil in a Oligofructose Overload Model |
title_sort | dynamic ros production and gene expression of heifers blood neutrophil in a oligofructose overload model |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7186304/ https://www.ncbi.nlm.nih.gov/pubmed/32373641 http://dx.doi.org/10.3389/fvets.2020.00211 |
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