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Longitudinal Study of Three microRNAs in Duchenne Muscular Dystrophy and Becker Muscular Dystrophy
Our objective was to investigate the potential of three microRNAs, miR-181a-5p, miR-30c-5p, and miR-206 as prognostic biomarkers for long-term follow up of Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD) patients. We analyzed the expression of three microRNAs in serum of 18 pat...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7186470/ https://www.ncbi.nlm.nih.gov/pubmed/32373058 http://dx.doi.org/10.3389/fneur.2020.00304 |
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author | Trifunov, Selena Natera-de Benito, Daniel Exposito Escudero, Jesica Maria Ortez, Carlos Medina, Julita Cuadras, Daniel Badosa, Carmen Carrera, Laura Nascimento, Andres Jimenez-Mallebrera, Cecilia |
author_facet | Trifunov, Selena Natera-de Benito, Daniel Exposito Escudero, Jesica Maria Ortez, Carlos Medina, Julita Cuadras, Daniel Badosa, Carmen Carrera, Laura Nascimento, Andres Jimenez-Mallebrera, Cecilia |
author_sort | Trifunov, Selena |
collection | PubMed |
description | Our objective was to investigate the potential of three microRNAs, miR-181a-5p, miR-30c-5p, and miR-206 as prognostic biomarkers for long-term follow up of Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD) patients. We analyzed the expression of three microRNAs in serum of 18 patients (DMD 13, BMD 5) and 13 controls using droplet digital PCR. Over 4 years a minimum of two and a maximum of three measurements were performed at different time points in the same patient. Correlations between microRNA serum levels, age, and functional outcome measures were analyzed. We show the individual evolution of the levels of the three microRNAs in 12 patients and also the effect of corticosteroid treatment on microRNAs expression. We measure the expression of three microRNAs in the muscle of six DMD patients and also the expression of target genes for miR-30c. We found that levels of miR-30c and miR-206 remained significantly elevated in DMD patients relative to controls over the entire study length. The introduction of the corticosteroid treatment did not significantly influence the levels of these microRNAs. We report a trend for microRNA levels to decrease with age. Moreover, miR-206 expression levels are capable to distinguish DMD from BMD patients according to ROC analysis. We found miR-30c expression decreased in the muscle of DMD patients and marked upregulation of the target genes for this microRNA. MiR-30c and miR-206 represent sensitive biomarkers for DMD, while miR-206 may have an additional value to distinguish the DMD and BMD phenotype. This may be particularly relevant to assess the effectiveness of treatments aimed at converting the DMD to the less-severe BMD like phenotype. |
format | Online Article Text |
id | pubmed-7186470 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71864702020-05-05 Longitudinal Study of Three microRNAs in Duchenne Muscular Dystrophy and Becker Muscular Dystrophy Trifunov, Selena Natera-de Benito, Daniel Exposito Escudero, Jesica Maria Ortez, Carlos Medina, Julita Cuadras, Daniel Badosa, Carmen Carrera, Laura Nascimento, Andres Jimenez-Mallebrera, Cecilia Front Neurol Neurology Our objective was to investigate the potential of three microRNAs, miR-181a-5p, miR-30c-5p, and miR-206 as prognostic biomarkers for long-term follow up of Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD) patients. We analyzed the expression of three microRNAs in serum of 18 patients (DMD 13, BMD 5) and 13 controls using droplet digital PCR. Over 4 years a minimum of two and a maximum of three measurements were performed at different time points in the same patient. Correlations between microRNA serum levels, age, and functional outcome measures were analyzed. We show the individual evolution of the levels of the three microRNAs in 12 patients and also the effect of corticosteroid treatment on microRNAs expression. We measure the expression of three microRNAs in the muscle of six DMD patients and also the expression of target genes for miR-30c. We found that levels of miR-30c and miR-206 remained significantly elevated in DMD patients relative to controls over the entire study length. The introduction of the corticosteroid treatment did not significantly influence the levels of these microRNAs. We report a trend for microRNA levels to decrease with age. Moreover, miR-206 expression levels are capable to distinguish DMD from BMD patients according to ROC analysis. We found miR-30c expression decreased in the muscle of DMD patients and marked upregulation of the target genes for this microRNA. MiR-30c and miR-206 represent sensitive biomarkers for DMD, while miR-206 may have an additional value to distinguish the DMD and BMD phenotype. This may be particularly relevant to assess the effectiveness of treatments aimed at converting the DMD to the less-severe BMD like phenotype. Frontiers Media S.A. 2020-04-21 /pmc/articles/PMC7186470/ /pubmed/32373058 http://dx.doi.org/10.3389/fneur.2020.00304 Text en Copyright © 2020 Trifunov, Natera-de Benito, Exposito Escudero, Ortez, Medina, Cuadras, Badosa, Carrera, Nascimento and Jimenez-Mallebrera. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neurology Trifunov, Selena Natera-de Benito, Daniel Exposito Escudero, Jesica Maria Ortez, Carlos Medina, Julita Cuadras, Daniel Badosa, Carmen Carrera, Laura Nascimento, Andres Jimenez-Mallebrera, Cecilia Longitudinal Study of Three microRNAs in Duchenne Muscular Dystrophy and Becker Muscular Dystrophy |
title | Longitudinal Study of Three microRNAs in Duchenne Muscular Dystrophy and Becker Muscular Dystrophy |
title_full | Longitudinal Study of Three microRNAs in Duchenne Muscular Dystrophy and Becker Muscular Dystrophy |
title_fullStr | Longitudinal Study of Three microRNAs in Duchenne Muscular Dystrophy and Becker Muscular Dystrophy |
title_full_unstemmed | Longitudinal Study of Three microRNAs in Duchenne Muscular Dystrophy and Becker Muscular Dystrophy |
title_short | Longitudinal Study of Three microRNAs in Duchenne Muscular Dystrophy and Becker Muscular Dystrophy |
title_sort | longitudinal study of three micrornas in duchenne muscular dystrophy and becker muscular dystrophy |
topic | Neurology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7186470/ https://www.ncbi.nlm.nih.gov/pubmed/32373058 http://dx.doi.org/10.3389/fneur.2020.00304 |
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